Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients
Objective. To analyze the methylation status of miR-124a loci in synovial tissues of rheumatoid arthritis (RA) patients using methylation-specific polymerase chain reaction (MSP). Materials and Methods. DNA obtained from the frozen tissue of 7 RA samples, 6 osteoarthritis (OA) samples, and 3 healthy...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/524204 |
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| _version_ | 1832565259563433984 |
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| author | Qiao Zhou Li Long Guixiu Shi Jing Zhang Tong Wu Bin Zhou |
| author_facet | Qiao Zhou Li Long Guixiu Shi Jing Zhang Tong Wu Bin Zhou |
| author_sort | Qiao Zhou |
| collection | DOAJ |
| description | Objective. To analyze the methylation status of miR-124a loci in synovial tissues of rheumatoid arthritis (RA) patients using methylation-specific polymerase chain reaction (MSP). Materials and Methods. DNA obtained from the frozen tissue of 7 RA samples, 6 osteoarthritis (OA) samples, and 3 healthy controls were undergoing bisulfite conversion and then analyzed for miR-124a promoter methylation using MSP assay. Results. miR-124-a1 and miR-124-a2 promoter methylation were both seen in 71.4% of RA samples compared to 16.7% of OA samples. miR-124-a3 promoter methylation was seen in 57.1% of RA samples and 0% of OA samples. All the three loci were unmethylated in 3 healthy controls. Conclusion. The methylation status of miR-124a seen in this study concurs with that reported in tumor cells, indicating epigenetic dysregulation constituents, a mechanism in the development of rheumatoid arthritis. |
| format | Article |
| id | doaj-art-c2654c58c23f464288aca3714a8fea75 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-c2654c58c23f464288aca3714a8fea752025-02-03T01:08:49ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/524204524204Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis PatientsQiao Zhou0Li Long1Guixiu Shi2Jing Zhang3Tong Wu4Bin Zhou5Department of Rheumatology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, No. 32 West Section 2, Yihuan Road, Chengdu, Sichuan 610072, ChinaDepartment of Rheumatology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, No. 32 West Section 2, Yihuan Road, Chengdu, Sichuan 610072, ChinaDepartment of Rheumatology, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, ChinaDepartment of Rheumatology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, No. 32 West Section 2, Yihuan Road, Chengdu, Sichuan 610072, ChinaDepartment of Rheumatology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, No. 32 West Section 2, Yihuan Road, Chengdu, Sichuan 610072, ChinaDepartment of Rheumatology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, No. 32 West Section 2, Yihuan Road, Chengdu, Sichuan 610072, ChinaObjective. To analyze the methylation status of miR-124a loci in synovial tissues of rheumatoid arthritis (RA) patients using methylation-specific polymerase chain reaction (MSP). Materials and Methods. DNA obtained from the frozen tissue of 7 RA samples, 6 osteoarthritis (OA) samples, and 3 healthy controls were undergoing bisulfite conversion and then analyzed for miR-124a promoter methylation using MSP assay. Results. miR-124-a1 and miR-124-a2 promoter methylation were both seen in 71.4% of RA samples compared to 16.7% of OA samples. miR-124-a3 promoter methylation was seen in 57.1% of RA samples and 0% of OA samples. All the three loci were unmethylated in 3 healthy controls. Conclusion. The methylation status of miR-124a seen in this study concurs with that reported in tumor cells, indicating epigenetic dysregulation constituents, a mechanism in the development of rheumatoid arthritis.http://dx.doi.org/10.1155/2013/524204 |
| spellingShingle | Qiao Zhou Li Long Guixiu Shi Jing Zhang Tong Wu Bin Zhou Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients Clinical and Developmental Immunology |
| title | Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients |
| title_full | Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients |
| title_fullStr | Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients |
| title_full_unstemmed | Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients |
| title_short | Research of the Methylation Status of miR-124a Gene Promoter among Rheumatoid Arthritis Patients |
| title_sort | research of the methylation status of mir 124a gene promoter among rheumatoid arthritis patients |
| url | http://dx.doi.org/10.1155/2013/524204 |
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