Cold Shock Proteins Balance Biofilm-Associated Antibiotic Resistance and Oxidative Vulnerability in Mycobacteria
Cold Shock Proteins (Csps) are multifunctional regulators critical for bacterial stress adaptation. While Csps are known to regulate biofilm formation and low-temperature growth in some species, their roles in mycobacteria remain unclear. Here, we explored the functions of three Csps (CspA1, CspA2,...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/7/1597 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849408965937463296 |
|---|---|
| author | Jiachen Zheng Linzhao He Yizhang Wei Jie Lu Xiaolin Liu Weihui Li |
| author_facet | Jiachen Zheng Linzhao He Yizhang Wei Jie Lu Xiaolin Liu Weihui Li |
| author_sort | Jiachen Zheng |
| collection | DOAJ |
| description | Cold Shock Proteins (Csps) are multifunctional regulators critical for bacterial stress adaptation. While Csps are known to regulate biofilm formation and low-temperature growth in some species, their roles in mycobacteria remain unclear. Here, we explored the functions of three Csps (CspA1, CspA2, and CspB) in <i>Mycobacterium smegmatis</i>. We found that CspA1 promotes biofilm formation and isoniazid (INH) resistance but negatively affects oxidative stress resistance. In contrast, CspB promotes biofilm formation, whereas CspA2 appears functionally redundant in this process. Notably, CspB and CspA2 do not contribute redundantly to oxidative stress resistance. Proteomic analysis revealed that CspA1 significantly modulates the expression of key metabolic and stress-response proteins, including WhiB3 and KatG. Our findings establish CspA1 as a key regulatory factor in mycobacteria, linking metabolic adaptation to biofilm-associated drug resistance and oxidative defense. |
| format | Article |
| id | doaj-art-c261d4bf20234d4ea0fe336d07a1cf8d |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-c261d4bf20234d4ea0fe336d07a1cf8d2025-08-20T03:35:38ZengMDPI AGMicroorganisms2076-26072025-07-01137159710.3390/microorganisms13071597Cold Shock Proteins Balance Biofilm-Associated Antibiotic Resistance and Oxidative Vulnerability in MycobacteriaJiachen Zheng0Linzhao He1Yizhang Wei2Jie Lu3Xiaolin Liu4Weihui Li5State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaCold Shock Proteins (Csps) are multifunctional regulators critical for bacterial stress adaptation. While Csps are known to regulate biofilm formation and low-temperature growth in some species, their roles in mycobacteria remain unclear. Here, we explored the functions of three Csps (CspA1, CspA2, and CspB) in <i>Mycobacterium smegmatis</i>. We found that CspA1 promotes biofilm formation and isoniazid (INH) resistance but negatively affects oxidative stress resistance. In contrast, CspB promotes biofilm formation, whereas CspA2 appears functionally redundant in this process. Notably, CspB and CspA2 do not contribute redundantly to oxidative stress resistance. Proteomic analysis revealed that CspA1 significantly modulates the expression of key metabolic and stress-response proteins, including WhiB3 and KatG. Our findings establish CspA1 as a key regulatory factor in mycobacteria, linking metabolic adaptation to biofilm-associated drug resistance and oxidative defense.https://www.mdpi.com/2076-2607/13/7/1597cold shock proteinbiofilmsoxidative stressisoniazid resistancetranscriptional regulation |
| spellingShingle | Jiachen Zheng Linzhao He Yizhang Wei Jie Lu Xiaolin Liu Weihui Li Cold Shock Proteins Balance Biofilm-Associated Antibiotic Resistance and Oxidative Vulnerability in Mycobacteria Microorganisms cold shock protein biofilms oxidative stress isoniazid resistance transcriptional regulation |
| title | Cold Shock Proteins Balance Biofilm-Associated Antibiotic Resistance and Oxidative Vulnerability in Mycobacteria |
| title_full | Cold Shock Proteins Balance Biofilm-Associated Antibiotic Resistance and Oxidative Vulnerability in Mycobacteria |
| title_fullStr | Cold Shock Proteins Balance Biofilm-Associated Antibiotic Resistance and Oxidative Vulnerability in Mycobacteria |
| title_full_unstemmed | Cold Shock Proteins Balance Biofilm-Associated Antibiotic Resistance and Oxidative Vulnerability in Mycobacteria |
| title_short | Cold Shock Proteins Balance Biofilm-Associated Antibiotic Resistance and Oxidative Vulnerability in Mycobacteria |
| title_sort | cold shock proteins balance biofilm associated antibiotic resistance and oxidative vulnerability in mycobacteria |
| topic | cold shock protein biofilms oxidative stress isoniazid resistance transcriptional regulation |
| url | https://www.mdpi.com/2076-2607/13/7/1597 |
| work_keys_str_mv | AT jiachenzheng coldshockproteinsbalancebiofilmassociatedantibioticresistanceandoxidativevulnerabilityinmycobacteria AT linzhaohe coldshockproteinsbalancebiofilmassociatedantibioticresistanceandoxidativevulnerabilityinmycobacteria AT yizhangwei coldshockproteinsbalancebiofilmassociatedantibioticresistanceandoxidativevulnerabilityinmycobacteria AT jielu coldshockproteinsbalancebiofilmassociatedantibioticresistanceandoxidativevulnerabilityinmycobacteria AT xiaolinliu coldshockproteinsbalancebiofilmassociatedantibioticresistanceandoxidativevulnerabilityinmycobacteria AT weihuili coldshockproteinsbalancebiofilmassociatedantibioticresistanceandoxidativevulnerabilityinmycobacteria |