Inhibition of Cervical Cancer Cell Migration by Human Wharton’s Jelly Stem Cells
Background: One of the most prevalent malignancies to strike women, both in Iran and globally, is cervical cancer. Metastasis, which is a significant cause of mortality, is one of the most significant pathological processes of this cancer. Therefore, preventing the migration of cancer cells may be a...
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Mazandaran University of Medical Sciences and Health Services
2022-05-01
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| Series: | Research in Molecular Medicine |
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| Online Access: | http://rmm.mazums.ac.ir/article-1-463-en.pdf |
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| author | Snwr Sabir Mohammad Masoud Maleki |
| author_facet | Snwr Sabir Mohammad Masoud Maleki |
| author_sort | Snwr Sabir Mohammad |
| collection | DOAJ |
| description | Background: One of the most prevalent malignancies to strike women, both in Iran and globally, is cervical cancer. Metastasis, which is a significant cause of mortality, is one of the most significant pathological processes of this cancer. Therefore, preventing the migration of cancer cells may be a useful therapeutic approach. The aim of this work was to investigate the impact of conditioned media and human Wharton's jelly stem cells (hWJSCs) on the migration and growth of the cervical cancer cell line Hela as well as the in vitro mRNA expression of genes involved in metastasis.
Methods: After primary culture, cellular extract and conditioned medium of hWJSCs were prepared. The viability of cervical cancer cells was investigated by MTT assay after treatment with cellular extract and conditioned medium of hWJSCs. Moreover, the anti-migratory effects of cellular extract and conditioned medium of hWJSCs on the cervical cancer cells were evaluated by wound‐healing migration assay. Finally, the mRNA expression of migration-related genes (E-cadherin and Vimentin) was detected by real-time PCR.
Results: Our results indicated that the cellular extract and conditioned medium of hWJSCs (with 32% concentration) inhibited the proliferation of 100% and 20% of Hela cancer cells, respectively. In addition, the cellular extract and conditioned medium of hWJSCs significantly decreased morphological alteration and migration of the cancer cells. The cellular extract and conditioned medium of hWJSCs modified the expression of Vimentin and E-cadherin genes to inhibit the cancer cell migration (p<0.05). However, the cellular extract indicated significantly profound inhibitory effects on the cervical cancer cells compared to the conditioned medium.
Conclusions: Our study demonstrated that the cellular extract and conditioned medium of hWJSCs inhibit the proliferation and migration of cervical cancer cells through the modification of migration-related gene expression. However, further in vitro and in vivo studies are required for more accurate results. |
| format | Article |
| id | doaj-art-c26023e05fff4be5b600100ca5de716e |
| institution | OA Journals |
| issn | 2322-1348 2322-133X |
| language | English |
| publishDate | 2022-05-01 |
| publisher | Mazandaran University of Medical Sciences and Health Services |
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| series | Research in Molecular Medicine |
| spelling | doaj-art-c26023e05fff4be5b600100ca5de716e2025-08-20T02:16:02ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2022-05-01102125132Inhibition of Cervical Cancer Cell Migration by Human Wharton’s Jelly Stem CellsSnwr Sabir Mohammad0Masoud Maleki1 Department of Molecular Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran Department of Molecular Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran Background: One of the most prevalent malignancies to strike women, both in Iran and globally, is cervical cancer. Metastasis, which is a significant cause of mortality, is one of the most significant pathological processes of this cancer. Therefore, preventing the migration of cancer cells may be a useful therapeutic approach. The aim of this work was to investigate the impact of conditioned media and human Wharton's jelly stem cells (hWJSCs) on the migration and growth of the cervical cancer cell line Hela as well as the in vitro mRNA expression of genes involved in metastasis. Methods: After primary culture, cellular extract and conditioned medium of hWJSCs were prepared. The viability of cervical cancer cells was investigated by MTT assay after treatment with cellular extract and conditioned medium of hWJSCs. Moreover, the anti-migratory effects of cellular extract and conditioned medium of hWJSCs on the cervical cancer cells were evaluated by wound‐healing migration assay. Finally, the mRNA expression of migration-related genes (E-cadherin and Vimentin) was detected by real-time PCR. Results: Our results indicated that the cellular extract and conditioned medium of hWJSCs (with 32% concentration) inhibited the proliferation of 100% and 20% of Hela cancer cells, respectively. In addition, the cellular extract and conditioned medium of hWJSCs significantly decreased morphological alteration and migration of the cancer cells. The cellular extract and conditioned medium of hWJSCs modified the expression of Vimentin and E-cadherin genes to inhibit the cancer cell migration (p<0.05). However, the cellular extract indicated significantly profound inhibitory effects on the cervical cancer cells compared to the conditioned medium. Conclusions: Our study demonstrated that the cellular extract and conditioned medium of hWJSCs inhibit the proliferation and migration of cervical cancer cells through the modification of migration-related gene expression. However, further in vitro and in vivo studies are required for more accurate results.http://rmm.mazums.ac.ir/article-1-463-en.pdfcervical cancermigrationwharton's jelly stem cellse-cadherinvimentin |
| spellingShingle | Snwr Sabir Mohammad Masoud Maleki Inhibition of Cervical Cancer Cell Migration by Human Wharton’s Jelly Stem Cells Research in Molecular Medicine cervical cancer migration wharton's jelly stem cells e-cadherin vimentin |
| title | Inhibition of Cervical Cancer Cell Migration by Human Wharton’s Jelly Stem Cells |
| title_full | Inhibition of Cervical Cancer Cell Migration by Human Wharton’s Jelly Stem Cells |
| title_fullStr | Inhibition of Cervical Cancer Cell Migration by Human Wharton’s Jelly Stem Cells |
| title_full_unstemmed | Inhibition of Cervical Cancer Cell Migration by Human Wharton’s Jelly Stem Cells |
| title_short | Inhibition of Cervical Cancer Cell Migration by Human Wharton’s Jelly Stem Cells |
| title_sort | inhibition of cervical cancer cell migration by human wharton s jelly stem cells |
| topic | cervical cancer migration wharton's jelly stem cells e-cadherin vimentin |
| url | http://rmm.mazums.ac.ir/article-1-463-en.pdf |
| work_keys_str_mv | AT snwrsabirmohammad inhibitionofcervicalcancercellmigrationbyhumanwhartonsjellystemcells AT masoudmaleki inhibitionofcervicalcancercellmigrationbyhumanwhartonsjellystemcells |