Inhibitory effect of doxycycline on mmp2/9 and its improvement in cardiac function and left ventricular remodeling after myocardial infarction in rats
The objective of this research was to investigate the inhibitory effect of doxycycline (DOX) on MMP2/9 and the improvement in cardiac function and left ventricular remodeling (LVR) after myocardial infarction (MI) in rats. For this purpose, thirty-six rats were randomly divided into control (group A...
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Kafkas University, Faculty of Veterinary Medicine
2025-05-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=3192 |
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| author | Fenli ZHANG Kun LI Yongchao ZHANG |
| author_facet | Fenli ZHANG Kun LI Yongchao ZHANG |
| author_sort | Fenli ZHANG |
| collection | DOAJ |
| description | The objective of this research was to investigate the inhibitory effect of doxycycline
(DOX) on MMP2/9 and the improvement in cardiac function and left ventricular
remodeling (LVR) after myocardial infarction (MI) in rats. For this purpose, thirty-six
rats were randomly divided into control (group A), model (group B) and experimental
(group C) groups. A MI model was established in groups B and C by ligation of the left
anterior descending (LAD) of the coronary artery, while the rats in group A underwent
thoracotomy without ligation only. The rats in groups A and B were injected with normal
saline, and the rats in group C were injected with DOX. Two weeks after the operation,
the cardiac function of the rats was evaluated by color Doppler ultrasound, myocardial
infarct size by Masson staining, collagen content and the ratio of type Ⅰ/III collagen by
immunohistochemistry and immunoblotting respectively and activities of MMP2/9 by
gelatin enzyme method. The results revealed DOX to cause reduction in the degree of
ventricular enlargement and cardiac wall thinning, reduce the collagen content, increase
the ratio of type Ⅰ/III collagen, decrease the activities of MMP2/9, and reduce myocardial
destruction and remodeling in rats after MI. However, further clinical research is
recommended for the evaluation of the effectiveness of DOX. |
| format | Article |
| id | doaj-art-c25fc38fab994dcea574a8ab1e558459 |
| institution | Kabale University |
| issn | 1309-2251 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj-art-c25fc38fab994dcea574a8ab1e5584592025-08-20T03:30:25ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512025-05-0131336136610.9775/kvfd.2024.333903192Inhibitory effect of doxycycline on mmp2/9 and its improvement in cardiac function and left ventricular remodeling after myocardial infarction in ratsFenli ZHANG0Kun LI1Yongchao ZHANG2Department of Structural Cardiology, First Affiliated Hospital of Xi'an Jiaotong University, Xi’an 710061, Shaanxi Province, CHINACollege of Veterinary Medicine, Nanjing Agricultural University, Nanjing, CHINAElectrocardiogram Room, Xi'an Fifth Hospital, Xi’an 710082, Shaanxi Province, CHINAThe objective of this research was to investigate the inhibitory effect of doxycycline (DOX) on MMP2/9 and the improvement in cardiac function and left ventricular remodeling (LVR) after myocardial infarction (MI) in rats. For this purpose, thirty-six rats were randomly divided into control (group A), model (group B) and experimental (group C) groups. A MI model was established in groups B and C by ligation of the left anterior descending (LAD) of the coronary artery, while the rats in group A underwent thoracotomy without ligation only. The rats in groups A and B were injected with normal saline, and the rats in group C were injected with DOX. Two weeks after the operation, the cardiac function of the rats was evaluated by color Doppler ultrasound, myocardial infarct size by Masson staining, collagen content and the ratio of type Ⅰ/III collagen by immunohistochemistry and immunoblotting respectively and activities of MMP2/9 by gelatin enzyme method. The results revealed DOX to cause reduction in the degree of ventricular enlargement and cardiac wall thinning, reduce the collagen content, increase the ratio of type Ⅰ/III collagen, decrease the activities of MMP2/9, and reduce myocardial destruction and remodeling in rats after MI. However, further clinical research is recommended for the evaluation of the effectiveness of DOX.https://vetdergikafkas.org/pdf.php?id=3192doxycyclineleft ventricular remodelingmyocardial infarctionmatrix metalloproteinases |
| spellingShingle | Fenli ZHANG Kun LI Yongchao ZHANG Inhibitory effect of doxycycline on mmp2/9 and its improvement in cardiac function and left ventricular remodeling after myocardial infarction in rats Kafkas Universitesi Veteriner Fakültesi Dergisi doxycycline left ventricular remodeling myocardial infarction matrix metalloproteinases |
| title | Inhibitory effect of doxycycline on mmp2/9 and its improvement in cardiac function and left ventricular remodeling after myocardial infarction in rats |
| title_full | Inhibitory effect of doxycycline on mmp2/9 and its improvement in cardiac function and left ventricular remodeling after myocardial infarction in rats |
| title_fullStr | Inhibitory effect of doxycycline on mmp2/9 and its improvement in cardiac function and left ventricular remodeling after myocardial infarction in rats |
| title_full_unstemmed | Inhibitory effect of doxycycline on mmp2/9 and its improvement in cardiac function and left ventricular remodeling after myocardial infarction in rats |
| title_short | Inhibitory effect of doxycycline on mmp2/9 and its improvement in cardiac function and left ventricular remodeling after myocardial infarction in rats |
| title_sort | inhibitory effect of doxycycline on mmp2 9 and its improvement in cardiac function and left ventricular remodeling after myocardial infarction in rats |
| topic | doxycycline left ventricular remodeling myocardial infarction matrix metalloproteinases |
| url | https://vetdergikafkas.org/pdf.php?id=3192 |
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