Search for a genetic cause of variably protease-sensitive prionopathy.

Variably protease-sensitive prionopathy (VPSPr) is a rare, atypical subtype of prion disease currently classified as sporadic. We performed exome sequencing and targeted sequencing of PRNP non-coding regions on genomic DNA from autopsy-confirmed VPSPr patients (N = 67) in order to search for a possi...

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Main Authors: Yuan Lian, Keisi Kotobelli, Stacey Hall, Michael E Talkowski, Anne O'Donnell-Luria, Sonia Minikel Vallabh, Brian S Appleby, Eric Vallabh Minikel
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-08-01
Series:PLoS Pathogens
Online Access:https://doi.org/10.1371/journal.ppat.1013343
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author Yuan Lian
Keisi Kotobelli
Stacey Hall
Michael E Talkowski
Anne O'Donnell-Luria
Sonia Minikel Vallabh
Brian S Appleby
Eric Vallabh Minikel
author_facet Yuan Lian
Keisi Kotobelli
Stacey Hall
Michael E Talkowski
Anne O'Donnell-Luria
Sonia Minikel Vallabh
Brian S Appleby
Eric Vallabh Minikel
author_sort Yuan Lian
collection DOAJ
description Variably protease-sensitive prionopathy (VPSPr) is a rare, atypical subtype of prion disease currently classified as sporadic. We performed exome sequencing and targeted sequencing of PRNP non-coding regions on genomic DNA from autopsy-confirmed VPSPr patients (N = 67) in order to search for a possible genetic cause. Our search identified no potentially causal variants for VPSPr. The common polymorphism PRNP M129V was the largest genetic risk factor for VPSPr, with an odds ratio of 7.0. Other variants in and near PRNP exhibited association to VPSPr risk only in proportion to their linkage disequilibrium with M129V, and upstream expression quantitative trait loci showed no evidence of independent association to VPSPr risk. We cannot rule out the possibility of causal variants hiding in genomic regions or classes of genetic variation that our search did not canvas. Nevertheless, our data support the classification of VPSPr as a sporadic prion disease.
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publishDate 2025-08-01
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spelling doaj-art-c259c615772f49f3b1f68636bee6489b2025-08-23T05:31:24ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742025-08-01218e101334310.1371/journal.ppat.1013343Search for a genetic cause of variably protease-sensitive prionopathy.Yuan LianKeisi KotobelliStacey HallMichael E TalkowskiAnne O'Donnell-LuriaSonia Minikel VallabhBrian S ApplebyEric Vallabh MinikelVariably protease-sensitive prionopathy (VPSPr) is a rare, atypical subtype of prion disease currently classified as sporadic. We performed exome sequencing and targeted sequencing of PRNP non-coding regions on genomic DNA from autopsy-confirmed VPSPr patients (N = 67) in order to search for a possible genetic cause. Our search identified no potentially causal variants for VPSPr. The common polymorphism PRNP M129V was the largest genetic risk factor for VPSPr, with an odds ratio of 7.0. Other variants in and near PRNP exhibited association to VPSPr risk only in proportion to their linkage disequilibrium with M129V, and upstream expression quantitative trait loci showed no evidence of independent association to VPSPr risk. We cannot rule out the possibility of causal variants hiding in genomic regions or classes of genetic variation that our search did not canvas. Nevertheless, our data support the classification of VPSPr as a sporadic prion disease.https://doi.org/10.1371/journal.ppat.1013343
spellingShingle Yuan Lian
Keisi Kotobelli
Stacey Hall
Michael E Talkowski
Anne O'Donnell-Luria
Sonia Minikel Vallabh
Brian S Appleby
Eric Vallabh Minikel
Search for a genetic cause of variably protease-sensitive prionopathy.
PLoS Pathogens
title Search for a genetic cause of variably protease-sensitive prionopathy.
title_full Search for a genetic cause of variably protease-sensitive prionopathy.
title_fullStr Search for a genetic cause of variably protease-sensitive prionopathy.
title_full_unstemmed Search for a genetic cause of variably protease-sensitive prionopathy.
title_short Search for a genetic cause of variably protease-sensitive prionopathy.
title_sort search for a genetic cause of variably protease sensitive prionopathy
url https://doi.org/10.1371/journal.ppat.1013343
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