Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes
The main objective of the present work was to prepare and assess dermal delivery of tacrolimus-loaded ethosomes versus classic liposomes. Both delivery systems were characterized for particle size, polydispersity index, and entrapment efficiency (EE), by dynamic laser diffraction and ultrafiltration...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/874053 |
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| author | Guiling Li Chao Fan Xinru Li Yating Fan Xiaoning Wang Mei Li Yan Liu |
| author_facet | Guiling Li Chao Fan Xinru Li Yating Fan Xiaoning Wang Mei Li Yan Liu |
| author_sort | Guiling Li |
| collection | DOAJ |
| description | The main objective of the present work was to prepare and assess dermal delivery of tacrolimus-loaded ethosomes versus classic liposomes. Both delivery systems were characterized for particle size, polydispersity index, and entrapment efficiency (EE), by dynamic laser diffraction and ultrafiltration or dialysis methods, respectively. The results indicated that presence of ethanol in the formulations affected the particle size. In addition, ultrafiltration method was selected to determine EE due to relatively short period as compared with dialysis method. Ethosomes exhibited a significant higher EE and amount of drug in dermis in contrast to classic liposomes suggesting that ethosomes with higher entrapment capacity prompted more amount of tacrolimus to permeate through stratum corneum and reach the target of atopic dermatitis (AD). Physical stability was very well for tacrolimus-loaded ethosomes under storage condition (4°C). Our results demonstrated that the ethosomal system might be a promising candidate for dermal delivery of tacrolimus for AD. |
| format | Article |
| id | doaj-art-c256a16aff0847a6a97e828131d98856 |
| institution | OA Journals |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-c256a16aff0847a6a97e828131d988562025-08-20T02:19:03ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/874053874053Preparation and In Vitro Evaluation of Tacrolimus-Loaded EthosomesGuiling Li0Chao Fan1Xinru Li2Yating Fan3Xiaoning Wang4Mei Li5Yan Liu6Institute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, ChinaDepartment of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaDepartment of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaDepartment of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaDepartment of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaInstitute of Medicinal Biotechnology, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100050, ChinaDepartment of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaThe main objective of the present work was to prepare and assess dermal delivery of tacrolimus-loaded ethosomes versus classic liposomes. Both delivery systems were characterized for particle size, polydispersity index, and entrapment efficiency (EE), by dynamic laser diffraction and ultrafiltration or dialysis methods, respectively. The results indicated that presence of ethanol in the formulations affected the particle size. In addition, ultrafiltration method was selected to determine EE due to relatively short period as compared with dialysis method. Ethosomes exhibited a significant higher EE and amount of drug in dermis in contrast to classic liposomes suggesting that ethosomes with higher entrapment capacity prompted more amount of tacrolimus to permeate through stratum corneum and reach the target of atopic dermatitis (AD). Physical stability was very well for tacrolimus-loaded ethosomes under storage condition (4°C). Our results demonstrated that the ethosomal system might be a promising candidate for dermal delivery of tacrolimus for AD.http://dx.doi.org/10.1100/2012/874053 |
| spellingShingle | Guiling Li Chao Fan Xinru Li Yating Fan Xiaoning Wang Mei Li Yan Liu Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes The Scientific World Journal |
| title | Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes |
| title_full | Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes |
| title_fullStr | Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes |
| title_full_unstemmed | Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes |
| title_short | Preparation and In Vitro Evaluation of Tacrolimus-Loaded Ethosomes |
| title_sort | preparation and in vitro evaluation of tacrolimus loaded ethosomes |
| url | http://dx.doi.org/10.1100/2012/874053 |
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