Use of polyclonal antibodies, electron microscopy and histopathology to detect iridovirus-like particles in bullfrogs
Iridoviruses of the Ranavirus genus have been implicated in the decline in amphibians worldwide, capable of affecting animals b o t h i n t h e w i l d a n d i n c a p t i v i t y . This study a i m e d to detect iridovirus-like particles from three frog farms in southeastern Brazil using primary p...
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Instituto de Pesca
2016-09-01
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| Series: | Boletim do Instituto de Pesca |
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| Online Access: | https://institutodepesca.org/index.php/bip/article/view/1155 |
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| author | Pedro Verdan NEVES Márcia Helena CATROXO Marcio HIPOLITO Cinthia Rodrigues de OLIVEIRA Cláudia Maris FERREIRA |
| author_facet | Pedro Verdan NEVES Márcia Helena CATROXO Marcio HIPOLITO Cinthia Rodrigues de OLIVEIRA Cláudia Maris FERREIRA |
| author_sort | Pedro Verdan NEVES |
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Iridoviruses of the Ranavirus genus have been implicated in the decline in amphibians worldwide, capable of affecting animals b o t h i n t h e w i l d a n d i n c a p t i v i t y . This study a i m e d to detect iridovirus-like particles from three frog farms in southeastern Brazil using primary polyclonal antibodies, transmission electron microscopy (TEM) and histologic findings. The target organs were liver and kidneys. Sixty adults and sixty tadpoles of bullfrogs (Lithobates catesbeianus) were used in the study. TEM revealed the presence of iridovirus-like particles in hepatic tissue using the negative staining technique. Positive results were also observed by immunoelectron microscopy and immunocytochemistry (ICC). The histological analysis o f t h e s a m p l e s s h o w e d liver hemorrhage and corpuscles inclusion in hepatocytes as well as glomerulotubular degeneration and necrosis in the kidneys. The methods used in this study were highly efficient to detect the presence of iridovirus-like particles and possible infection of ranavirus.
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| format | Article |
| id | doaj-art-c24250a8e1294cfda80bd4bf695ac770 |
| institution | DOAJ |
| issn | 1678-2305 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | Instituto de Pesca |
| record_format | Article |
| series | Boletim do Instituto de Pesca |
| spelling | doaj-art-c24250a8e1294cfda80bd4bf695ac7702025-08-20T03:04:53ZengInstituto de PescaBoletim do Instituto de Pesca1678-23052016-09-0142310.20950/1678-2305.2016v42n3p523Use of polyclonal antibodies, electron microscopy and histopathology to detect iridovirus-like particles in bullfrogsPedro Verdan NEVES0Márcia Helena CATROXO1Marcio HIPOLITO2Cinthia Rodrigues de OLIVEIRA3Cláudia Maris FERREIRA4Fisheries Institute - APTA - SAABiological Institute - APTA - SAABiological Institute - APTA - SAAFisheries Institute - APTA - SAAFisheries Institute - APTA - SAA Iridoviruses of the Ranavirus genus have been implicated in the decline in amphibians worldwide, capable of affecting animals b o t h i n t h e w i l d a n d i n c a p t i v i t y . This study a i m e d to detect iridovirus-like particles from three frog farms in southeastern Brazil using primary polyclonal antibodies, transmission electron microscopy (TEM) and histologic findings. The target organs were liver and kidneys. Sixty adults and sixty tadpoles of bullfrogs (Lithobates catesbeianus) were used in the study. TEM revealed the presence of iridovirus-like particles in hepatic tissue using the negative staining technique. Positive results were also observed by immunoelectron microscopy and immunocytochemistry (ICC). The histological analysis o f t h e s a m p l e s s h o w e d liver hemorrhage and corpuscles inclusion in hepatocytes as well as glomerulotubular degeneration and necrosis in the kidneys. The methods used in this study were highly efficient to detect the presence of iridovirus-like particles and possible infection of ranavirus. https://institutodepesca.org/index.php/bip/article/view/1155Rana catesbeianaFV3immunoelectron microscopyranavirus |
| spellingShingle | Pedro Verdan NEVES Márcia Helena CATROXO Marcio HIPOLITO Cinthia Rodrigues de OLIVEIRA Cláudia Maris FERREIRA Use of polyclonal antibodies, electron microscopy and histopathology to detect iridovirus-like particles in bullfrogs Boletim do Instituto de Pesca Rana catesbeiana FV3 immunoelectron microscopy ranavirus |
| title | Use of polyclonal antibodies, electron microscopy and histopathology to detect iridovirus-like particles in bullfrogs |
| title_full | Use of polyclonal antibodies, electron microscopy and histopathology to detect iridovirus-like particles in bullfrogs |
| title_fullStr | Use of polyclonal antibodies, electron microscopy and histopathology to detect iridovirus-like particles in bullfrogs |
| title_full_unstemmed | Use of polyclonal antibodies, electron microscopy and histopathology to detect iridovirus-like particles in bullfrogs |
| title_short | Use of polyclonal antibodies, electron microscopy and histopathology to detect iridovirus-like particles in bullfrogs |
| title_sort | use of polyclonal antibodies electron microscopy and histopathology to detect iridovirus like particles in bullfrogs |
| topic | Rana catesbeiana FV3 immunoelectron microscopy ranavirus |
| url | https://institutodepesca.org/index.php/bip/article/view/1155 |
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