Endophytic bacterial communities colonizing the medicinal plant Calotropis procera: as resources of hydrolases

Calotropis procera (Aiton) W.T. Aiton is a shrub belongs to family Asclepiadaceae which known by its medicinal properties. It is a widely growing plant distributed in tropical and sub-tropical Africa, and America. This study is the first report which highlights the diversity of bacterial endophyte...

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Main Authors: Fatma M. Abdel Baset, Noura Sh. A. Hagaggi, Francis F. Hezayen, Usama M. Abdul- Raouf
Format: Article
Language:English
Published: ResearchersLinks, Ltd 2020-12-01
Series:Novel Research in Microbiology Journal
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Online Access:https://nrmj.journals.ekb.eg/article_130852_b66690ae925bc99591826d085315525f.pdf
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author Fatma M. Abdel Baset
Noura Sh. A. Hagaggi
Francis F. Hezayen
Usama M. Abdul- Raouf
author_facet Fatma M. Abdel Baset
Noura Sh. A. Hagaggi
Francis F. Hezayen
Usama M. Abdul- Raouf
author_sort Fatma M. Abdel Baset
collection DOAJ
description Calotropis procera (Aiton) W.T. Aiton is a shrub belongs to family Asclepiadaceae which known by its medicinal properties. It is a widely growing plant distributed in tropical and sub-tropical Africa, and America. This study is the first report which highlights the diversity of bacterial endophytes from C. procera as sources of numerous hydrolytic exo-enzymes. Endophytic bacteria were isolated from all plant parts such as; roots, stems, leaves, flowers, fruits and latex. Bacillus was the prevalent genus. At the species level, the bacterial diversity was high. Eight representative species were isolated including; Citricoccus alkalitolerans (Cps2) (NR025771), Bacillus cereus (Cps1) (NR074540), B. pumilus (Cps3) (NR112637), B. firmus (Cpl1) (NR025842), B. niabensis (Cpl3) (NR043334), B. subtilis (Cpl4) (NR113265), B. amyloliquefaciens (Cpl10) (NR041455) and B. subtilis subsp. spizizenii (Cpl13) (NR112686). Results of the current study emphasized that C. procera plant hosts diverse endophytic bacteria, which are potential producers of several economically important hydrolytic enzymes i.e., amylase, protease, cellulase, lipase and L-asparaginase. The aims of the current study were to identify the endophytic bacteria associated with the different organs of the medicinal plant C. procera, and to evaluate their potentialities to produce diverse extracellular hydrolytic enzymes.
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spelling doaj-art-c23cda5a0a0c4b62a4c0a564fba3d4832025-08-20T03:23:27ZengResearchersLinks, LtdNovel Research in Microbiology Journal2537-02862537-02942020-12-01461045105610.21608/nrmj.2020.130852Endophytic bacterial communities colonizing the medicinal plant Calotropis procera: as resources of hydrolasesFatma M. Abdel Baset0Noura Sh. A. Hagaggi1Francis F. Hezayen2 Usama M. Abdul- Raouf3Botany Department, Faculty of Science, Aswan University, 81528 Aswan, EgyptBotany Department, Faculty of Science, Aswan University, 81528 Aswan, EgyptBotany Department, Faculty of Science, Aswan University, 81528 Aswan, EgyptBotany Department, Faculty of Science, Aswan University, 81528 Aswan, EgyptCalotropis procera (Aiton) W.T. Aiton is a shrub belongs to family Asclepiadaceae which known by its medicinal properties. It is a widely growing plant distributed in tropical and sub-tropical Africa, and America. This study is the first report which highlights the diversity of bacterial endophytes from C. procera as sources of numerous hydrolytic exo-enzymes. Endophytic bacteria were isolated from all plant parts such as; roots, stems, leaves, flowers, fruits and latex. Bacillus was the prevalent genus. At the species level, the bacterial diversity was high. Eight representative species were isolated including; Citricoccus alkalitolerans (Cps2) (NR025771), Bacillus cereus (Cps1) (NR074540), B. pumilus (Cps3) (NR112637), B. firmus (Cpl1) (NR025842), B. niabensis (Cpl3) (NR043334), B. subtilis (Cpl4) (NR113265), B. amyloliquefaciens (Cpl10) (NR041455) and B. subtilis subsp. spizizenii (Cpl13) (NR112686). Results of the current study emphasized that C. procera plant hosts diverse endophytic bacteria, which are potential producers of several economically important hydrolytic enzymes i.e., amylase, protease, cellulase, lipase and L-asparaginase. The aims of the current study were to identify the endophytic bacteria associated with the different organs of the medicinal plant C. procera, and to evaluate their potentialities to produce diverse extracellular hydrolytic enzymes.https://nrmj.journals.ekb.eg/article_130852_b66690ae925bc99591826d085315525f.pdfendophytic bacteriacalotropis procerahydrolasesenzymes
spellingShingle Fatma M. Abdel Baset
Noura Sh. A. Hagaggi
Francis F. Hezayen
Usama M. Abdul- Raouf
Endophytic bacterial communities colonizing the medicinal plant Calotropis procera: as resources of hydrolases
Novel Research in Microbiology Journal
endophytic bacteria
calotropis procera
hydrolases
enzymes
title Endophytic bacterial communities colonizing the medicinal plant Calotropis procera: as resources of hydrolases
title_full Endophytic bacterial communities colonizing the medicinal plant Calotropis procera: as resources of hydrolases
title_fullStr Endophytic bacterial communities colonizing the medicinal plant Calotropis procera: as resources of hydrolases
title_full_unstemmed Endophytic bacterial communities colonizing the medicinal plant Calotropis procera: as resources of hydrolases
title_short Endophytic bacterial communities colonizing the medicinal plant Calotropis procera: as resources of hydrolases
title_sort endophytic bacterial communities colonizing the medicinal plant calotropis procera as resources of hydrolases
topic endophytic bacteria
calotropis procera
hydrolases
enzymes
url https://nrmj.journals.ekb.eg/article_130852_b66690ae925bc99591826d085315525f.pdf
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AT francisfhezayen endophyticbacterialcommunitiescolonizingthemedicinalplantcalotropisproceraasresourcesofhydrolases
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