Carbapenemase-producing Pseudomonas aeruginosa isolated from patients exhibited multidrug resistance in Sokoto, Nigeria
Introduction: Carbapenemase-producing P. aeruginosa is one of the top priority pathogens responsible for treatment failures in cystic fibrosis, abscess and soft tissue infections. The study aimed to determine the prevalence of carbapenemase-producing P. aeruginosa in patients visiting two major publ...
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Elsevier
2025-09-01
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| Series: | Medicine in Microecology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590097825000151 |
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| author | Hassan Bawa Kabiru Mohammed Abdulrazak Nuhu Abdulmalik Shuaibu Farhan Rhidor Akorede Olalekan Adesola Muhammad Bashir Bello Abdurrahman Hassan Jibril |
| author_facet | Hassan Bawa Kabiru Mohammed Abdulrazak Nuhu Abdulmalik Shuaibu Farhan Rhidor Akorede Olalekan Adesola Muhammad Bashir Bello Abdurrahman Hassan Jibril |
| author_sort | Hassan Bawa |
| collection | DOAJ |
| description | Introduction: Carbapenemase-producing P. aeruginosa is one of the top priority pathogens responsible for treatment failures in cystic fibrosis, abscess and soft tissue infections. The study aimed to determine the prevalence of carbapenemase-producing P. aeruginosa in patients visiting two major public hospitals in Sokoto, Nigeria. Methods: Two hundred and four samples were collected from in- and outpatients attending two major public hospitals in Sokoto, Nigeria. Identifying P. aeruginosa involved culturing on cetrimide agar and confirming by PCR detection of the oprI and oprL genes specific to P. aeruginosa. Isolates were screened for sensitivity to ten antibiotics using the Kirby-Bauer disc diffusion test. To identify carbapenemase production, isolates resistant to meropenem and imipenem were further screened using the combined disc synergy test. Molecular detection of carbapenemase was done using PCR detection of blaVIM, blaKPC and blaIMP genes. Results: Out of the 204 samples collected, 26 (12.7 %) of P. aeruginosa were recovered. All 26 isolates demonstrated resistance to more than three classes of antibiotics tested. Notably, 19.2 % (5/26) of the isolates were pan-drug-resistant to all antibiotics tested. Besides, 65.4 % (14/26) of isolates were at least resistant to ampicillin, kanamycin, cefotaxime, trimethoprim and chloramphenicol. A combined disk synergy test showed six isolates to produce carbapenemase enzyme. Likewise, blaVIM and blaIMP were detected in all of these isolates, while blaKPC was not detected in any. Conclusions: The presence of carbapenemase-producing P. aeruginosa (carrying blaVIM and blaIMP genes) among hospital patients in Sokoto and the high antibiotic resistance detected represent a challenging threat to public health. Further research is crucial to understanding this carbapenemase gene's transmission source and developing effective strategies to combat it. |
| format | Article |
| id | doaj-art-c22d0f226c5546eba7ed2f5835e4710c |
| institution | Kabale University |
| issn | 2590-0978 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
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| series | Medicine in Microecology |
| spelling | doaj-art-c22d0f226c5546eba7ed2f5835e4710c2025-08-20T04:03:25ZengElsevierMedicine in Microecology2590-09782025-09-012510013410.1016/j.medmic.2025.100134Carbapenemase-producing Pseudomonas aeruginosa isolated from patients exhibited multidrug resistance in Sokoto, NigeriaHassan Bawa0Kabiru Mohammed1Abdulrazak Nuhu2Abdulmalik Shuaibu3Farhan Rhidor Akorede4Olalekan Adesola5Muhammad Bashir Bello6Abdurrahman Hassan Jibril7Department of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University Sokoto, Nigeria; Medical Laboratory Council of Nigeria, Sokoto Zonal Office, NigeriaDepartment of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University Sokoto, NigeriaDepartment of Medical Microbiology, School of Medical Laboratory Science, Usmanu Danfodiyo University Sokoto, NigeriaDepartment of Veterinary Microbiology, Usmanu Danfodiyo University Sokoto, NigeriaDepartment of Veterinary Pharmacology and Toxicology, Usmanu Danfodiyo University Sokoto, NigeriaDepartment of Medical Laboratory Science, Faculty of Basic Medical Sciences, University of Lagos, PMB 12003, Lagos, NigeriaDepartment of Veterinary Microbiology, Usmanu Danfodiyo University Sokoto, Nigeria; Infectious Disease Research Department, King Abdullah International Medical Research Center, Riyadh, Saudi Arabia; Centre for Advanced Medical Research and Training, Usmanu Danfodiyo University Sokoto, Nigeria; One Health Institute, Usmanu Danfodiyo University Sokoto, NigeriaCentre for Advanced Medical Research and Training, Usmanu Danfodiyo University Sokoto, Nigeria; One Health Institute, Usmanu Danfodiyo University Sokoto, Nigeria; Department of Veterinary Public Health and Preventive Medicine, Usmanu Danfodiyo University Sokoto, Nigeria; Corresponding author. Department of Veterinary Public Health and Preventive Medicine, Usmanu Danfodiyo University Sokoto, Nigeria.Introduction: Carbapenemase-producing P. aeruginosa is one of the top priority pathogens responsible for treatment failures in cystic fibrosis, abscess and soft tissue infections. The study aimed to determine the prevalence of carbapenemase-producing P. aeruginosa in patients visiting two major public hospitals in Sokoto, Nigeria. Methods: Two hundred and four samples were collected from in- and outpatients attending two major public hospitals in Sokoto, Nigeria. Identifying P. aeruginosa involved culturing on cetrimide agar and confirming by PCR detection of the oprI and oprL genes specific to P. aeruginosa. Isolates were screened for sensitivity to ten antibiotics using the Kirby-Bauer disc diffusion test. To identify carbapenemase production, isolates resistant to meropenem and imipenem were further screened using the combined disc synergy test. Molecular detection of carbapenemase was done using PCR detection of blaVIM, blaKPC and blaIMP genes. Results: Out of the 204 samples collected, 26 (12.7 %) of P. aeruginosa were recovered. All 26 isolates demonstrated resistance to more than three classes of antibiotics tested. Notably, 19.2 % (5/26) of the isolates were pan-drug-resistant to all antibiotics tested. Besides, 65.4 % (14/26) of isolates were at least resistant to ampicillin, kanamycin, cefotaxime, trimethoprim and chloramphenicol. A combined disk synergy test showed six isolates to produce carbapenemase enzyme. Likewise, blaVIM and blaIMP were detected in all of these isolates, while blaKPC was not detected in any. Conclusions: The presence of carbapenemase-producing P. aeruginosa (carrying blaVIM and blaIMP genes) among hospital patients in Sokoto and the high antibiotic resistance detected represent a challenging threat to public health. Further research is crucial to understanding this carbapenemase gene's transmission source and developing effective strategies to combat it.http://www.sciencedirect.com/science/article/pii/S2590097825000151NigeriaPseudomonas aeruginosaMulti-drug resistanceCarbapenemaseVIMIMP |
| spellingShingle | Hassan Bawa Kabiru Mohammed Abdulrazak Nuhu Abdulmalik Shuaibu Farhan Rhidor Akorede Olalekan Adesola Muhammad Bashir Bello Abdurrahman Hassan Jibril Carbapenemase-producing Pseudomonas aeruginosa isolated from patients exhibited multidrug resistance in Sokoto, Nigeria Medicine in Microecology Nigeria Pseudomonas aeruginosa Multi-drug resistance Carbapenemase VIM IMP |
| title | Carbapenemase-producing Pseudomonas aeruginosa isolated from patients exhibited multidrug resistance in Sokoto, Nigeria |
| title_full | Carbapenemase-producing Pseudomonas aeruginosa isolated from patients exhibited multidrug resistance in Sokoto, Nigeria |
| title_fullStr | Carbapenemase-producing Pseudomonas aeruginosa isolated from patients exhibited multidrug resistance in Sokoto, Nigeria |
| title_full_unstemmed | Carbapenemase-producing Pseudomonas aeruginosa isolated from patients exhibited multidrug resistance in Sokoto, Nigeria |
| title_short | Carbapenemase-producing Pseudomonas aeruginosa isolated from patients exhibited multidrug resistance in Sokoto, Nigeria |
| title_sort | carbapenemase producing pseudomonas aeruginosa isolated from patients exhibited multidrug resistance in sokoto nigeria |
| topic | Nigeria Pseudomonas aeruginosa Multi-drug resistance Carbapenemase VIM IMP |
| url | http://www.sciencedirect.com/science/article/pii/S2590097825000151 |
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