MOFs—Combining Fully Synthetic Injectable Hydrogel Scaffolds Exhibiting Higher Skeletal Muscle Regenerative Efficiency than Matrigel
Due to its sarcoma-derived origin and the associated carcinogenic risks, as well as its lack of tissue-specific extracellular matrix biochemical cues, the use of the injectable gel scaffold Matrigel is generally restricted to research applications. Therefore, the development of new fully synthetic i...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Gels |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2310-2861/11/7/514 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850078155460575232 |
|---|---|
| author | Sobuj Shahidul Islam Tatsuya Dode Soma Kawashima Myu Fukuoka Takaaki Tsuruoka Koji Nagahama |
| author_facet | Sobuj Shahidul Islam Tatsuya Dode Soma Kawashima Myu Fukuoka Takaaki Tsuruoka Koji Nagahama |
| author_sort | Sobuj Shahidul Islam |
| collection | DOAJ |
| description | Due to its sarcoma-derived origin and the associated carcinogenic risks, as well as its lack of tissue-specific extracellular matrix biochemical cues, the use of the injectable gel scaffold Matrigel is generally restricted to research applications. Therefore, the development of new fully synthetic injectable gel scaffolds that exhibit performance comparable to Matrigel is a high priority. In this study, we developed a novel fully synthetic injectable gel scaffold by combining a biodegradable PLGA-PEG-PLGA copolymer, clay nanoparticle LAPONITE®, and L-arginine-loaded metal–organic frameworks (NU-1000) at the nano level. An aqueous solution of the developed hybrid scaffold (PLGA-PEG-PLGA/LAPONITE®/L-Arg@NU-1000) exhibited rapid sol–gel transition at body temperature following simple injection and formed a continuous bulk-sized gel, demonstrating good injectability. Long-term sustained slow release of L-arginine from the resultant gels can be achieved because NU-1000 is a suitable reservoir for L-arginine. PLGA-PEG-PLGA/LAPONITE®/L-Arg@NU-1000 hybrid gels exhibited good compatibility with and promoted the growth of human skeletal muscle satellite cells. Importantly, in vivo experiments using skeletal muscle injury model mice demonstrated that the tissue regeneration efficiency of PLGA-PEG-PLGA/LAPONITE®/L-Arg@NU-1000 gels is higher than that of Matrigel. Specifically, we judged the higher tissue regeneration efficacy of our gels by histological analysis, including MYH3 immunofluorescent staining, H&E staining, and Masson’s trichrome staining. Taken together, these data suggest that novel hybrid hydrogels could serve as injectable hydrogel scaffolds for in vivo tissue engineering and ultimately replace Matrigel. |
| format | Article |
| id | doaj-art-c22c240cd51b49ebb7add4c10c560e83 |
| institution | DOAJ |
| issn | 2310-2861 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj-art-c22c240cd51b49ebb7add4c10c560e832025-08-20T02:45:38ZengMDPI AGGels2310-28612025-07-0111751410.3390/gels11070514MOFs—Combining Fully Synthetic Injectable Hydrogel Scaffolds Exhibiting Higher Skeletal Muscle Regenerative Efficiency than MatrigelSobuj Shahidul Islam0Tatsuya Dode1Soma Kawashima2Myu Fukuoka3Takaaki Tsuruoka4Koji Nagahama5Department of Nanobiochemistry, Frontiers of Innovative Research on Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, JapanDepartment of Nanobiochemistry, Frontiers of Innovative Research on Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, JapanDepartment of Nanobiochemistry, Frontiers of Innovative Research on Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, JapanDepartment of Nanobiochemistry, Frontiers of Innovative Research on Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, JapanDepartment of Nanobiochemistry, Frontiers of Innovative Research on Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, JapanDepartment of Nanobiochemistry, Frontiers of Innovative Research on Science and Technology (FIRST), Konan University, 7-1-20 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, JapanDue to its sarcoma-derived origin and the associated carcinogenic risks, as well as its lack of tissue-specific extracellular matrix biochemical cues, the use of the injectable gel scaffold Matrigel is generally restricted to research applications. Therefore, the development of new fully synthetic injectable gel scaffolds that exhibit performance comparable to Matrigel is a high priority. In this study, we developed a novel fully synthetic injectable gel scaffold by combining a biodegradable PLGA-PEG-PLGA copolymer, clay nanoparticle LAPONITE®, and L-arginine-loaded metal–organic frameworks (NU-1000) at the nano level. An aqueous solution of the developed hybrid scaffold (PLGA-PEG-PLGA/LAPONITE®/L-Arg@NU-1000) exhibited rapid sol–gel transition at body temperature following simple injection and formed a continuous bulk-sized gel, demonstrating good injectability. Long-term sustained slow release of L-arginine from the resultant gels can be achieved because NU-1000 is a suitable reservoir for L-arginine. PLGA-PEG-PLGA/LAPONITE®/L-Arg@NU-1000 hybrid gels exhibited good compatibility with and promoted the growth of human skeletal muscle satellite cells. Importantly, in vivo experiments using skeletal muscle injury model mice demonstrated that the tissue regeneration efficiency of PLGA-PEG-PLGA/LAPONITE®/L-Arg@NU-1000 gels is higher than that of Matrigel. Specifically, we judged the higher tissue regeneration efficacy of our gels by histological analysis, including MYH3 immunofluorescent staining, H&E staining, and Masson’s trichrome staining. Taken together, these data suggest that novel hybrid hydrogels could serve as injectable hydrogel scaffolds for in vivo tissue engineering and ultimately replace Matrigel.https://www.mdpi.com/2310-2861/11/7/514injectable hydrogelsMOFsscaffoldstissue engineeringskeletal muscle tissue |
| spellingShingle | Sobuj Shahidul Islam Tatsuya Dode Soma Kawashima Myu Fukuoka Takaaki Tsuruoka Koji Nagahama MOFs—Combining Fully Synthetic Injectable Hydrogel Scaffolds Exhibiting Higher Skeletal Muscle Regenerative Efficiency than Matrigel Gels injectable hydrogels MOFs scaffolds tissue engineering skeletal muscle tissue |
| title | MOFs—Combining Fully Synthetic Injectable Hydrogel Scaffolds Exhibiting Higher Skeletal Muscle Regenerative Efficiency than Matrigel |
| title_full | MOFs—Combining Fully Synthetic Injectable Hydrogel Scaffolds Exhibiting Higher Skeletal Muscle Regenerative Efficiency than Matrigel |
| title_fullStr | MOFs—Combining Fully Synthetic Injectable Hydrogel Scaffolds Exhibiting Higher Skeletal Muscle Regenerative Efficiency than Matrigel |
| title_full_unstemmed | MOFs—Combining Fully Synthetic Injectable Hydrogel Scaffolds Exhibiting Higher Skeletal Muscle Regenerative Efficiency than Matrigel |
| title_short | MOFs—Combining Fully Synthetic Injectable Hydrogel Scaffolds Exhibiting Higher Skeletal Muscle Regenerative Efficiency than Matrigel |
| title_sort | mofs combining fully synthetic injectable hydrogel scaffolds exhibiting higher skeletal muscle regenerative efficiency than matrigel |
| topic | injectable hydrogels MOFs scaffolds tissue engineering skeletal muscle tissue |
| url | https://www.mdpi.com/2310-2861/11/7/514 |
| work_keys_str_mv | AT sobujshahidulislam mofscombiningfullysyntheticinjectablehydrogelscaffoldsexhibitinghigherskeletalmuscleregenerativeefficiencythanmatrigel AT tatsuyadode mofscombiningfullysyntheticinjectablehydrogelscaffoldsexhibitinghigherskeletalmuscleregenerativeefficiencythanmatrigel AT somakawashima mofscombiningfullysyntheticinjectablehydrogelscaffoldsexhibitinghigherskeletalmuscleregenerativeefficiencythanmatrigel AT myufukuoka mofscombiningfullysyntheticinjectablehydrogelscaffoldsexhibitinghigherskeletalmuscleregenerativeefficiencythanmatrigel AT takaakitsuruoka mofscombiningfullysyntheticinjectablehydrogelscaffoldsexhibitinghigherskeletalmuscleregenerativeefficiencythanmatrigel AT kojinagahama mofscombiningfullysyntheticinjectablehydrogelscaffoldsexhibitinghigherskeletalmuscleregenerativeefficiencythanmatrigel |