Immunohistochemistry for assessing toxicity and mechanism of action of anticancer drugs during preclinical trials. From theory to practice
Aim. To identify the most suitable pathogenetic mechanisms for in-depth study of the antitumor and antimetastatic effects of tested hybrid organotin compounds using the immunohistochemical approach.Materials and Methods. Here, we tested bis(3,5-di-tert-butyl-4-hydroxyphenylthiolate) dimethyltin (lab...
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| Format: | Article |
| Language: | Russian |
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Kemerovo State Medical University
2024-09-01
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| Series: | Фундаментальная и клиническая медицина |
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| Online Access: | https://fcm.kemsmu.ru/jour/article/view/903 |
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| author | M. A. Dodokhova M. A. Akimenko O. V. Voronova M. S. Alkhusein-Kulyaginova N. A. Kornienko M. V. Gulyan D. N. Gyulmamedov M.-M. Kh. Alasheva E. Sh. Kazimagomedova D. B. Shpakovsky E. R. Milaeva I. M. Kotieva |
| author_facet | M. A. Dodokhova M. A. Akimenko O. V. Voronova M. S. Alkhusein-Kulyaginova N. A. Kornienko M. V. Gulyan D. N. Gyulmamedov M.-M. Kh. Alasheva E. Sh. Kazimagomedova D. B. Shpakovsky E. R. Milaeva I. M. Kotieva |
| author_sort | M. A. Dodokhova |
| collection | DOAJ |
| description | Aim. To identify the most suitable pathogenetic mechanisms for in-depth study of the antitumor and antimetastatic effects of tested hybrid organotin compounds using the immunohistochemical approach.Materials and Methods. Here, we tested bis(3,5-di-tert-butyl-4-hydroxyphenylthiolate) dimethyltin (laboratory code Me-3), belonging to the class of hybrid organotin compounds, on 30 female C57Bl/6 mice using a universal model of transplantable tumors with spontaneous metastasis (B16 melanoma). 48 hours after tumor cell transplantation, we intraperitoneally administered Me-3 once daily to female C57Bl/6 mice for 10 days at a total dose (TD) of 375 mg/kg. For histological analysis, we used the primary tumor node of B16 melanoma. Immunophenotyping of B16 melanoma tissue samples was carried out using the polyclonal antibodies to transforming growth factor beta 1 (TGFβ-1), vascular endothelial growth factor A (VEGFA), Bcl2-associated X Protein (Bcl-2), cluster of differentiation 34 (CD34).Results. After the exposure to Me-3, we found a reduced immunohistochemical signal to TGF-β1 and Bcl-2 3 in the tumor tissue. Low doses of Me-3 have also impacted angiogenesis.Conclusion. Me-3 has a pro-apoptotic and anti-angiogenetic effects on B16 melanoma cells in C57Bl/6 mice. |
| format | Article |
| id | doaj-art-c22a6a9bfd4f416b8aa4576e31242467 |
| institution | Kabale University |
| issn | 2500-0764 2542-0941 |
| language | Russian |
| publishDate | 2024-09-01 |
| publisher | Kemerovo State Medical University |
| record_format | Article |
| series | Фундаментальная и клиническая медицина |
| spelling | doaj-art-c22a6a9bfd4f416b8aa4576e312424672025-08-20T03:57:40ZrusKemerovo State Medical UniversityФундаментальная и клиническая медицина2500-07642542-09412024-09-0193748510.23946/2500-0764-2024-9-3-74-85442Immunohistochemistry for assessing toxicity and mechanism of action of anticancer drugs during preclinical trials. From theory to practiceM. A. Dodokhova0M. A. Akimenko1O. V. Voronova2M. S. Alkhusein-Kulyaginova3N. A. Kornienko4M. V. Gulyan5D. N. Gyulmamedov6M.-M. Kh. Alasheva7E. Sh. Kazimagomedova8D. B. Shpakovsky9E. R. Milaeva10I. M. Kotieva11Rostov State Medical UniversityRostov State Medical UniversityRegional Department of PathologyRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityRostov State Medical UniversityLomonosov Moscow State UniversityLomonosov Moscow State UniversityRostov State Medical UniversityAim. To identify the most suitable pathogenetic mechanisms for in-depth study of the antitumor and antimetastatic effects of tested hybrid organotin compounds using the immunohistochemical approach.Materials and Methods. Here, we tested bis(3,5-di-tert-butyl-4-hydroxyphenylthiolate) dimethyltin (laboratory code Me-3), belonging to the class of hybrid organotin compounds, on 30 female C57Bl/6 mice using a universal model of transplantable tumors with spontaneous metastasis (B16 melanoma). 48 hours after tumor cell transplantation, we intraperitoneally administered Me-3 once daily to female C57Bl/6 mice for 10 days at a total dose (TD) of 375 mg/kg. For histological analysis, we used the primary tumor node of B16 melanoma. Immunophenotyping of B16 melanoma tissue samples was carried out using the polyclonal antibodies to transforming growth factor beta 1 (TGFβ-1), vascular endothelial growth factor A (VEGFA), Bcl2-associated X Protein (Bcl-2), cluster of differentiation 34 (CD34).Results. After the exposure to Me-3, we found a reduced immunohistochemical signal to TGF-β1 and Bcl-2 3 in the tumor tissue. Low doses of Me-3 have also impacted angiogenesis.Conclusion. Me-3 has a pro-apoptotic and anti-angiogenetic effects on B16 melanoma cells in C57Bl/6 mice.https://fcm.kemsmu.ru/jour/article/view/903preclinical studiesantitumor drugsimmunohistochemistryangiogenesishybrid organotin compounds |
| spellingShingle | M. A. Dodokhova M. A. Akimenko O. V. Voronova M. S. Alkhusein-Kulyaginova N. A. Kornienko M. V. Gulyan D. N. Gyulmamedov M.-M. Kh. Alasheva E. Sh. Kazimagomedova D. B. Shpakovsky E. R. Milaeva I. M. Kotieva Immunohistochemistry for assessing toxicity and mechanism of action of anticancer drugs during preclinical trials. From theory to practice Фундаментальная и клиническая медицина preclinical studies antitumor drugs immunohistochemistry angiogenesis hybrid organotin compounds |
| title | Immunohistochemistry for assessing toxicity and mechanism of action of anticancer drugs during preclinical trials. From theory to practice |
| title_full | Immunohistochemistry for assessing toxicity and mechanism of action of anticancer drugs during preclinical trials. From theory to practice |
| title_fullStr | Immunohistochemistry for assessing toxicity and mechanism of action of anticancer drugs during preclinical trials. From theory to practice |
| title_full_unstemmed | Immunohistochemistry for assessing toxicity and mechanism of action of anticancer drugs during preclinical trials. From theory to practice |
| title_short | Immunohistochemistry for assessing toxicity and mechanism of action of anticancer drugs during preclinical trials. From theory to practice |
| title_sort | immunohistochemistry for assessing toxicity and mechanism of action of anticancer drugs during preclinical trials from theory to practice |
| topic | preclinical studies antitumor drugs immunohistochemistry angiogenesis hybrid organotin compounds |
| url | https://fcm.kemsmu.ru/jour/article/view/903 |
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