Age related gut microbiota regulates energy-related metabolism to influence natural aging phenotypes in the heart

As the population ages, problems pertaining to health and life expectancy due to the aging heart have become increasingly prominent. The gut microbiota has become a potential therapeutic target in several diseases, including cardiovascular diseases. Current studies on the roles of the gut microbiota...

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Main Authors: Shufen Wu, Lingran Qiao, Haiyan Liu, Yan-Li Li, Rui Wang, Yiru Yin, Enhui Li, Lele Wang, Xiaoya Guan, Litian Yin, Qinghua Liu, Xiaoyang Peng, Yutong Zhang, Zhuanfang Yang, Lin Zuo, Ce Zhang
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Experimental Gerontology
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Online Access:http://www.sciencedirect.com/science/article/pii/S0531556525000634
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author Shufen Wu
Lingran Qiao
Haiyan Liu
Yan-Li Li
Rui Wang
Yiru Yin
Enhui Li
Lele Wang
Xiaoya Guan
Litian Yin
Qinghua Liu
Xiaoyang Peng
Yutong Zhang
Zhuanfang Yang
Lin Zuo
Ce Zhang
author_facet Shufen Wu
Lingran Qiao
Haiyan Liu
Yan-Li Li
Rui Wang
Yiru Yin
Enhui Li
Lele Wang
Xiaoya Guan
Litian Yin
Qinghua Liu
Xiaoyang Peng
Yutong Zhang
Zhuanfang Yang
Lin Zuo
Ce Zhang
author_sort Shufen Wu
collection DOAJ
description As the population ages, problems pertaining to health and life expectancy due to the aging heart have become increasingly prominent. The gut microbiota has become a potential therapeutic target in several diseases, including cardiovascular diseases. Current studies on the roles of the gut microbiota in the cardiovascular system have focused mainly on cardiovascular diseases; therefore, the effects of the gut microbiota on the natural aging of myocardial tissue remain unclear. The present study aimed to explore the roles and mechanisms of the gut microbiota and related metabolites in the natural aging of the heart. Animal models of fecal microbiota transplantation (FMT) were established in elderly and young rats. 16S rRNA sequencing revealed that the gut microbiota of the recipients shifted toward the profile of the donors, with concomitant cardiac structure and diastolic function changes detected via ultrasound and positron emission tomography–computed tomography (PET–CT). A group of significantly enriched myocardial metabolites detected by LC/MS were involved in the fatty acid β-oxidation process. Together with altered glucose uptake, as revealed by PET–CT, changes in ATP content and mitochondrial structure further verified a metabolic difference related to energy among rats transplanted with the gut microbiota from donors of different ages. This study demonstrated that gut microbes may participate in the physiological aging process of the rat heart by regulating oxidative stress and autophagy. The gut microbiota has been shown to be involved in the natural aging of the heart at multiple levels, from the organ level to the metabolically plastic myocardiocytes and associated molecules.
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spelling doaj-art-c22465db9fbf4bf2be787a7abeefb93b2025-08-20T01:51:35ZengElsevierExperimental Gerontology1873-68152025-05-0120311273410.1016/j.exger.2025.112734Age related gut microbiota regulates energy-related metabolism to influence natural aging phenotypes in the heartShufen Wu0Lingran Qiao1Haiyan Liu2Yan-Li Li3Rui Wang4Yiru Yin5Enhui Li6Lele Wang7Xiaoya Guan8Litian Yin9Qinghua Liu10Xiaoyang Peng11Yutong Zhang12Zhuanfang Yang13Lin Zuo14Ce Zhang15Key Laboratory of Cellular Physiology, Ministry of Education, Department of Physiology, Shanxi Medical University, Taiyuan, China; Department of Pediatrics, Shanxi Medical University, Taiyuan, ChinaKey Laboratory of Cellular Physiology, Ministry of Education, Department of Physiology, Shanxi Medical University, Taiyuan, ChinaDepartment of Nuclear Medicine, First Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Neurology, First Hospital of Shanxi Medical University, Taiyuan, ChinaDepartment of Neurology, First Hospital of Shanxi Medical University, Taiyuan, ChinaKey Laboratory of Cellular Physiology, Ministry of Education, Department of Physiology, Shanxi Medical University, Taiyuan, China; Translational Medicine Research Center of Shanxi Medical University, Taiyuan, ChinaKey Laboratory of Cellular Physiology, Ministry of Education, Department of Physiology, Shanxi Medical University, Taiyuan, ChinaDepartment of Pediatrics, Shanxi Medical University, Taiyuan, ChinaShanxi Key Laboratory of Otorhinolaryngology Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan, China; Shanxi Province Clinical Medical Research Center for Precision Medicine of Head and Neck Cancer, First Hospital of Shanxi Medical University, Taiyuan, ChinaKey Laboratory of Cellular Physiology, Ministry of Education, Department of Physiology, Shanxi Medical University, Taiyuan, ChinaTranslational Medicine Research Center of Shanxi Medical University, Taiyuan, ChinaTranslational Medicine Research Center of Shanxi Medical University, Taiyuan, ChinaBasic Medical School, Shanxi Medical University, Taiyuan, ChinaKey Laboratory of Cellular Physiology, Ministry of Education, Department of Physiology, Shanxi Medical University, Taiyuan, ChinaKey Laboratory of Cellular Physiology, Ministry of Education, Department of Physiology, Shanxi Medical University, Taiyuan, China; Corresponding authors at: Key Laboratory of Cellular Physiology, Ministry of Education, Department of Physiology, Shanxi Medical University, Taiyuan 030001, China.Key Laboratory of Cellular Physiology, Ministry of Education, Department of Physiology, Shanxi Medical University, Taiyuan, China; Corresponding authors at: Key Laboratory of Cellular Physiology, Ministry of Education, Department of Physiology, Shanxi Medical University, Taiyuan 030001, China.As the population ages, problems pertaining to health and life expectancy due to the aging heart have become increasingly prominent. The gut microbiota has become a potential therapeutic target in several diseases, including cardiovascular diseases. Current studies on the roles of the gut microbiota in the cardiovascular system have focused mainly on cardiovascular diseases; therefore, the effects of the gut microbiota on the natural aging of myocardial tissue remain unclear. The present study aimed to explore the roles and mechanisms of the gut microbiota and related metabolites in the natural aging of the heart. Animal models of fecal microbiota transplantation (FMT) were established in elderly and young rats. 16S rRNA sequencing revealed that the gut microbiota of the recipients shifted toward the profile of the donors, with concomitant cardiac structure and diastolic function changes detected via ultrasound and positron emission tomography–computed tomography (PET–CT). A group of significantly enriched myocardial metabolites detected by LC/MS were involved in the fatty acid β-oxidation process. Together with altered glucose uptake, as revealed by PET–CT, changes in ATP content and mitochondrial structure further verified a metabolic difference related to energy among rats transplanted with the gut microbiota from donors of different ages. This study demonstrated that gut microbes may participate in the physiological aging process of the rat heart by regulating oxidative stress and autophagy. The gut microbiota has been shown to be involved in the natural aging of the heart at multiple levels, from the organ level to the metabolically plastic myocardiocytes and associated molecules.http://www.sciencedirect.com/science/article/pii/S0531556525000634AgingFecal microbiota transplantationGut microbiotaHeartMetabolism
spellingShingle Shufen Wu
Lingran Qiao
Haiyan Liu
Yan-Li Li
Rui Wang
Yiru Yin
Enhui Li
Lele Wang
Xiaoya Guan
Litian Yin
Qinghua Liu
Xiaoyang Peng
Yutong Zhang
Zhuanfang Yang
Lin Zuo
Ce Zhang
Age related gut microbiota regulates energy-related metabolism to influence natural aging phenotypes in the heart
Experimental Gerontology
Aging
Fecal microbiota transplantation
Gut microbiota
Heart
Metabolism
title Age related gut microbiota regulates energy-related metabolism to influence natural aging phenotypes in the heart
title_full Age related gut microbiota regulates energy-related metabolism to influence natural aging phenotypes in the heart
title_fullStr Age related gut microbiota regulates energy-related metabolism to influence natural aging phenotypes in the heart
title_full_unstemmed Age related gut microbiota regulates energy-related metabolism to influence natural aging phenotypes in the heart
title_short Age related gut microbiota regulates energy-related metabolism to influence natural aging phenotypes in the heart
title_sort age related gut microbiota regulates energy related metabolism to influence natural aging phenotypes in the heart
topic Aging
Fecal microbiota transplantation
Gut microbiota
Heart
Metabolism
url http://www.sciencedirect.com/science/article/pii/S0531556525000634
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