Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partners
The combined expression of a handful of pluripotency transcription factors (PluriTFs) in somatic cells can generate induced pluripotent stem cells (iPSCs). Here, we report the structural characterization of disordered regions contained in four important PluriTFs, namely Oct4, Sox2, Nanog and Esrrb....
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| Format: | Article |
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Académie des sciences
2024-04-01
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| Series: | Comptes Rendus. Chimie |
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| Online Access: | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.272/ |
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| author | Bouguechtouli, Chafiaa Ghouil, Rania Alik, Ania Dingli, Florent Loew, Damarys Theillet, Francois-Xavier |
| author_facet | Bouguechtouli, Chafiaa Ghouil, Rania Alik, Ania Dingli, Florent Loew, Damarys Theillet, Francois-Xavier |
| author_sort | Bouguechtouli, Chafiaa |
| collection | DOAJ |
| description | The combined expression of a handful of pluripotency transcription factors (PluriTFs) in somatic cells can generate induced pluripotent stem cells (iPSCs). Here, we report the structural characterization of disordered regions contained in four important PluriTFs, namely Oct4, Sox2, Nanog and Esrrb. Moreover, many post-translational modifications (PTMs) have been detected on PluriTFs, whose roles are not yet characterized. To help in their study, we also present a method (i) to produce well-characterized phosphorylation states of PluriTFs, using NMR analysis, and (ii) to use them for pull-downs in stem cell extracts analyzed by quantitative proteomics to detect potential Sox2 binders. |
| format | Article |
| id | doaj-art-c2220a505e96403ea3d2900adf1a77f5 |
| institution | Kabale University |
| issn | 1878-1543 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Académie des sciences |
| record_format | Article |
| series | Comptes Rendus. Chimie |
| spelling | doaj-art-c2220a505e96403ea3d2900adf1a77f52025-02-07T13:38:54ZengAcadémie des sciencesComptes Rendus. Chimie1878-15432024-04-0126S311913710.5802/crchim.27210.5802/crchim.272Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partnersBouguechtouli, Chafiaa0Ghouil, Rania1https://orcid.org/0000-0003-1628-0249Alik, Ania2Dingli, Florent3https://orcid.org/0000-0002-7715-2446Loew, Damarys4https://orcid.org/0000-0002-9111-8842Theillet, Francois-Xavier5https://orcid.org/0000-0002-3264-210XUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, FranceUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, FranceUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, FranceInstitut Curie, PSL Research University, Centre de Recherche, CurieCoreTech Spectrométrie de Masse Protéomique, Paris cedex 05, FranceInstitut Curie, PSL Research University, Centre de Recherche, CurieCoreTech Spectrométrie de Masse Protéomique, Paris cedex 05, FranceUniversité Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, FranceThe combined expression of a handful of pluripotency transcription factors (PluriTFs) in somatic cells can generate induced pluripotent stem cells (iPSCs). Here, we report the structural characterization of disordered regions contained in four important PluriTFs, namely Oct4, Sox2, Nanog and Esrrb. Moreover, many post-translational modifications (PTMs) have been detected on PluriTFs, whose roles are not yet characterized. To help in their study, we also present a method (i) to produce well-characterized phosphorylation states of PluriTFs, using NMR analysis, and (ii) to use them for pull-downs in stem cell extracts analyzed by quantitative proteomics to detect potential Sox2 binders.https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.272/Pluripotency transcription factorsIntrinsically disordered proteinsPost-translational modificationsKinasesNMRProteomicsQuantitative mass spectrometry |
| spellingShingle | Bouguechtouli, Chafiaa Ghouil, Rania Alik, Ania Dingli, Florent Loew, Damarys Theillet, Francois-Xavier Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partners Comptes Rendus. Chimie Pluripotency transcription factors Intrinsically disordered proteins Post-translational modifications Kinases NMR Proteomics Quantitative mass spectrometry |
| title | Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partners |
| title_full | Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partners |
| title_fullStr | Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partners |
| title_full_unstemmed | Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partners |
| title_short | Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partners |
| title_sort | structural characterization of stem cell factors oct4 sox2 nanog and esrrb disordered domains and a method to detect phospho dependent binding partners |
| topic | Pluripotency transcription factors Intrinsically disordered proteins Post-translational modifications Kinases NMR Proteomics Quantitative mass spectrometry |
| url | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.272/ |
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