Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partners

Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partners

The combined expression of a handful of pluripotency transcription factors (PluriTFs) in somatic cells can generate induced pluripotent stem cells (iPSCs). Here, we report the structural characterization of disordered regions contained in four important PluriTFs, namely Oct4, Sox2, Nanog and Esrrb....

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Bibliographic Details
Main Authors: Bouguechtouli, Chafiaa, Ghouil, Rania, Alik, Ania, Dingli, Florent, Loew, Damarys, Theillet, Francois-Xavier
Format: Article
Language:English
Published: Académie des sciences 2024-04-01
Series:Comptes Rendus. Chimie
Subjects:
Pluripotency transcription factors
Intrinsically disordered proteins
Post-translational modifications
Kinases
NMR
Proteomics
Quantitative mass spectrometry
Online Access:https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.272/
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Summary:The combined expression of a handful of pluripotency transcription factors (PluriTFs) in somatic cells can generate induced pluripotent stem cells (iPSCs). Here, we report the structural characterization of disordered regions contained in four important PluriTFs, namely Oct4, Sox2, Nanog and Esrrb. Moreover, many post-translational modifications (PTMs) have been detected on PluriTFs, whose roles are not yet characterized. To help in their study, we also present a method (i) to produce well-characterized phosphorylation states of PluriTFs, using NMR analysis, and (ii) to use them for pull-downs in stem cell extracts analyzed by quantitative proteomics to detect potential Sox2 binders.
ISSN:1878-1543

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