Injectable Biopolymer-hydroxyapatite Hydrogels: Obtaining and their Characterization
Hydrogels based on hydroxyapatite (HA) and Chitosan (CS) with addition of sodium alginate (Alg) were synthesized by in situ precipitation method. Structure, morphology, chemical and phase composition of the HA/CS and HA/CS/Alg hydrogels were characterized by TEM, FTIR and XRD. Hydrogels consist of l...
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Sumy State University
2016-03-01
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| Series: | Журнал нано- та електронної фізики |
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| Online Access: | http://jnep.sumdu.edu.ua/download/numbers/2016/1/articles/jnep_2016_V8_01032.pdf |
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| author | L.B. Sukhodub G.O. Yanovska V.M. Kuznetsov O.O. Martynyuk L.F. Sukhodub |
| author_facet | L.B. Sukhodub G.O. Yanovska V.M. Kuznetsov O.O. Martynyuk L.F. Sukhodub |
| author_sort | L.B. Sukhodub |
| collection | DOAJ |
| description | Hydrogels based on hydroxyapatite (HA) and Chitosan (CS) with addition of sodium alginate (Alg) were synthesized by in situ precipitation method. Structure, morphology, chemical and phase composition of the HA/CS and HA/CS/Alg hydrogels were characterized by TEM, FTIR and XRD. Hydrogels consist of low crystallinity calcium deficient hydroxyapatite (JCPDS 9 432), the needle-like crystallites have an average size 25 nm. The introduction of Alginate powder into HA/CS hydrogel solution demonstrate the viscosity enhancing of the HA/CS hydrogel due to polyelectrolyte reaction between Alginate and Chitosan macromolecules. Two natural polymers and partially released from hydroxyapatite Ca2+ ions formed a matrix by crosslinking the polymer macromolecules through hydroxyl, amino and carbonyl groups. These processes promote the formation of a more stable structure of HA/CS/Alg hydrogel as compared to HA/CS. The structural integrity and degradation tests have demonstrated that HA/CS/Alg1.0 saved its initial shape in 7 days of shaking in SBF solution, meanwhile for HA/CS, a structural decay was observed. The HA/CS hydrogel had completely lost its volume support after 1 day shaking in SBF. Thus, the ability of HA/CS hydrogel to maintain its shape with implantation into bone tissue defect may be enhanced with alginate addition, but alginate content more than 1 w/w % reduces the hydrogel plasticity, increases the swelling and accelerates the shape decay. |
| format | Article |
| id | doaj-art-c20bf8304f7f435dab64e98d2b5f0487 |
| institution | DOAJ |
| issn | 2077-6772 |
| language | English |
| publishDate | 2016-03-01 |
| publisher | Sumy State University |
| record_format | Article |
| series | Журнал нано- та електронної фізики |
| spelling | doaj-art-c20bf8304f7f435dab64e98d2b5f04872025-08-20T03:05:03ZengSumy State UniversityЖурнал нано- та електронної фізики2077-67722016-03-018101032-101032-8Injectable Biopolymer-hydroxyapatite Hydrogels: Obtaining and their CharacterizationL.B. Sukhodub0G.O. Yanovska1V.M. Kuznetsov2O.O. Martynyuk3L.F. Sukhodub4SI "Institute of Microbiology and Immunology, the Mechnikov I.I. AMS of Ukraine", 14/16, Pushkinska St., 61057 Kharkiv, UkraineSumy State University, 2, Rymskogo-Korsakova St., 40007 Sumy, UkraineSumy State University, 2, Rymskogo-Korsakova St., 40007 Sumy, UkraineSumy State University, 2, Rymskogo-Korsakova St., 40007 Sumy, UkraineSumy State University, 2, Rymskogo-Korsakova St., 40007 Sumy, UkraineHydrogels based on hydroxyapatite (HA) and Chitosan (CS) with addition of sodium alginate (Alg) were synthesized by in situ precipitation method. Structure, morphology, chemical and phase composition of the HA/CS and HA/CS/Alg hydrogels were characterized by TEM, FTIR and XRD. Hydrogels consist of low crystallinity calcium deficient hydroxyapatite (JCPDS 9 432), the needle-like crystallites have an average size 25 nm. The introduction of Alginate powder into HA/CS hydrogel solution demonstrate the viscosity enhancing of the HA/CS hydrogel due to polyelectrolyte reaction between Alginate and Chitosan macromolecules. Two natural polymers and partially released from hydroxyapatite Ca2+ ions formed a matrix by crosslinking the polymer macromolecules through hydroxyl, amino and carbonyl groups. These processes promote the formation of a more stable structure of HA/CS/Alg hydrogel as compared to HA/CS. The structural integrity and degradation tests have demonstrated that HA/CS/Alg1.0 saved its initial shape in 7 days of shaking in SBF solution, meanwhile for HA/CS, a structural decay was observed. The HA/CS hydrogel had completely lost its volume support after 1 day shaking in SBF. Thus, the ability of HA/CS hydrogel to maintain its shape with implantation into bone tissue defect may be enhanced with alginate addition, but alginate content more than 1 w/w % reduces the hydrogel plasticity, increases the swelling and accelerates the shape decay.http://jnep.sumdu.edu.ua/download/numbers/2016/1/articles/jnep_2016_V8_01032.pdfHydroxyapatiteChitosanSodium alginateHydrogel |
| spellingShingle | L.B. Sukhodub G.O. Yanovska V.M. Kuznetsov O.O. Martynyuk L.F. Sukhodub Injectable Biopolymer-hydroxyapatite Hydrogels: Obtaining and their Characterization Журнал нано- та електронної фізики Hydroxyapatite Chitosan Sodium alginate Hydrogel |
| title | Injectable Biopolymer-hydroxyapatite Hydrogels: Obtaining and their Characterization |
| title_full | Injectable Biopolymer-hydroxyapatite Hydrogels: Obtaining and their Characterization |
| title_fullStr | Injectable Biopolymer-hydroxyapatite Hydrogels: Obtaining and their Characterization |
| title_full_unstemmed | Injectable Biopolymer-hydroxyapatite Hydrogels: Obtaining and their Characterization |
| title_short | Injectable Biopolymer-hydroxyapatite Hydrogels: Obtaining and their Characterization |
| title_sort | injectable biopolymer hydroxyapatite hydrogels obtaining and their characterization |
| topic | Hydroxyapatite Chitosan Sodium alginate Hydrogel |
| url | http://jnep.sumdu.edu.ua/download/numbers/2016/1/articles/jnep_2016_V8_01032.pdf |
| work_keys_str_mv | AT lbsukhodub injectablebiopolymerhydroxyapatitehydrogelsobtainingandtheircharacterization AT goyanovska injectablebiopolymerhydroxyapatitehydrogelsobtainingandtheircharacterization AT vmkuznetsov injectablebiopolymerhydroxyapatitehydrogelsobtainingandtheircharacterization AT oomartynyuk injectablebiopolymerhydroxyapatitehydrogelsobtainingandtheircharacterization AT lfsukhodub injectablebiopolymerhydroxyapatitehydrogelsobtainingandtheircharacterization |