Revealing the Existence of Diverse Strategies for Phosphorus Solubilization and Acquisition in Plant-Growth Promoting <i>Streptomyces misionensis</i> SwB1
Phosphorus deficiency poses a significant challenge to plant growth and development, particularly in red soil. To alleviate this limitation, phosphorus-solubilizing bacteria (PSB) play a crucial role by converting insoluble phosphates present in the soil into soluble forms that are accessible to pla...
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2025-02-01
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| author | Yunzhu Chen Zhuangzhuang Gao Yan Yang Qiang Liu Lijuan Jiang Jingzhen Chen Xiao Zhou Luhong Zhang Yuena Ji Jia Tu Zhihong Xiao Peiwang Li Changzhu Li |
| author_facet | Yunzhu Chen Zhuangzhuang Gao Yan Yang Qiang Liu Lijuan Jiang Jingzhen Chen Xiao Zhou Luhong Zhang Yuena Ji Jia Tu Zhihong Xiao Peiwang Li Changzhu Li |
| author_sort | Yunzhu Chen |
| collection | DOAJ |
| description | Phosphorus deficiency poses a significant challenge to plant growth and development, particularly in red soil. To alleviate this limitation, phosphorus-solubilizing bacteria (PSB) play a crucial role by converting insoluble phosphates present in the soil into soluble forms that are accessible to plants. <i>Cornus wilsoniana</i> Wangerin is a representative oil crop cultivated in red soil, holding a prominent position within China’s forestry economic system. Consequently, it is essential to develop highly stable microbial phosphorus enhancement strategies to manage agricultural phosphorus in red soil regions, thereby maintaining the available phosphorus content necessary for the production of <i>C. wilsoniana</i>. In this study, the application of <i>Streptomyces misionensis</i> SwB1 bacterial suspension to the rhizosphere of <i>C. wilsoniana</i> significantly increased the content of various phosphorus fractions (H<sub>2</sub>O-P, NaHCO<sub>3</sub>-P, NaOH-P, HCl-P) in red soil, with NaHCO<sub>3</sub>-P content increasing by 4.97 times and NaOH-P content by 3.87 times. Additionally, the genome of <i>S. misionensis</i> SwB1 contains 25 phosphorus-solubilizing genes, 13 nitrogen-fixing genes, 17 siderophore production genes, and 11 indole-3-acetic acid (IAA) production genes, indicating its potential for enhancing nutrient availability. Comparative genomic analysis of 15 strains belonging to five species of <i>Streptomyces</i> revealed that <i>S. misionensis</i> SwB1 possesses an extensive genetic repertoire and complete gene clusters associated with phosphorus solubilization. Furthermore, five phosphorus solubilization pathways of <i>S. misionensis</i> SwB1 were summarized: the Pst system, Pit system, siderophore transport, phosphatase synthesis, and organic acid synthesis. Ultimately, the inoculation of <i>S. misionensis</i> SwB1 significantly enhanced the growth and biomass accumulation of <i>C. wilsoniana</i> at the seedling stage, evidenced by an increase in fresh weight by 81.44%, a rise in net photosynthetic rate by 18.51%, and a surge in the number of root tips by 36.24%. Taken together, our findings support a sophisticated multi-pathway bacteria phosphorus solubilization approach and identified a highly efficient phosphorus-solubilizing strain, <i>S. misionensis</i> SwB1, which has the potential to become a microbial fertilizer. |
| format | Article |
| id | doaj-art-c1f76d8f39264d31aec58bca7161c75a |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-c1f76d8f39264d31aec58bca7161c75a2025-08-20T03:12:15ZengMDPI AGMicroorganisms2076-26072025-02-0113237810.3390/microorganisms13020378Revealing the Existence of Diverse Strategies for Phosphorus Solubilization and Acquisition in Plant-Growth Promoting <i>Streptomyces misionensis</i> SwB1Yunzhu Chen0Zhuangzhuang Gao1Yan Yang2Qiang Liu3Lijuan Jiang4Jingzhen Chen5Xiao Zhou6Luhong Zhang7Yuena Ji8Jia Tu9Zhihong Xiao10Peiwang Li11Changzhu Li12State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, ChinaState Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, ChinaState Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, ChinaCollege of Life Science and Technology, Central South University of Forestry and Technology, 498 South Shaoshan Road, Changsha 410004, ChinaCollege of Life Science and Technology, Central South University of Forestry and Technology, 498 South Shaoshan Road, Changsha 410004, ChinaState Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, ChinaState Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, ChinaState Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, ChinaState Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, ChinaState Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, ChinaState Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, ChinaState Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, ChinaState Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, ChinaPhosphorus deficiency poses a significant challenge to plant growth and development, particularly in red soil. To alleviate this limitation, phosphorus-solubilizing bacteria (PSB) play a crucial role by converting insoluble phosphates present in the soil into soluble forms that are accessible to plants. <i>Cornus wilsoniana</i> Wangerin is a representative oil crop cultivated in red soil, holding a prominent position within China’s forestry economic system. Consequently, it is essential to develop highly stable microbial phosphorus enhancement strategies to manage agricultural phosphorus in red soil regions, thereby maintaining the available phosphorus content necessary for the production of <i>C. wilsoniana</i>. In this study, the application of <i>Streptomyces misionensis</i> SwB1 bacterial suspension to the rhizosphere of <i>C. wilsoniana</i> significantly increased the content of various phosphorus fractions (H<sub>2</sub>O-P, NaHCO<sub>3</sub>-P, NaOH-P, HCl-P) in red soil, with NaHCO<sub>3</sub>-P content increasing by 4.97 times and NaOH-P content by 3.87 times. Additionally, the genome of <i>S. misionensis</i> SwB1 contains 25 phosphorus-solubilizing genes, 13 nitrogen-fixing genes, 17 siderophore production genes, and 11 indole-3-acetic acid (IAA) production genes, indicating its potential for enhancing nutrient availability. Comparative genomic analysis of 15 strains belonging to five species of <i>Streptomyces</i> revealed that <i>S. misionensis</i> SwB1 possesses an extensive genetic repertoire and complete gene clusters associated with phosphorus solubilization. Furthermore, five phosphorus solubilization pathways of <i>S. misionensis</i> SwB1 were summarized: the Pst system, Pit system, siderophore transport, phosphatase synthesis, and organic acid synthesis. Ultimately, the inoculation of <i>S. misionensis</i> SwB1 significantly enhanced the growth and biomass accumulation of <i>C. wilsoniana</i> at the seedling stage, evidenced by an increase in fresh weight by 81.44%, a rise in net photosynthetic rate by 18.51%, and a surge in the number of root tips by 36.24%. Taken together, our findings support a sophisticated multi-pathway bacteria phosphorus solubilization approach and identified a highly efficient phosphorus-solubilizing strain, <i>S. misionensis</i> SwB1, which has the potential to become a microbial fertilizer.https://www.mdpi.com/2076-2607/13/2/378phosphate solubilizerphosphorus mobilization mechanismcomparative genomics<i>Cornus wilsoniana</i>microbial fertilizer |
| spellingShingle | Yunzhu Chen Zhuangzhuang Gao Yan Yang Qiang Liu Lijuan Jiang Jingzhen Chen Xiao Zhou Luhong Zhang Yuena Ji Jia Tu Zhihong Xiao Peiwang Li Changzhu Li Revealing the Existence of Diverse Strategies for Phosphorus Solubilization and Acquisition in Plant-Growth Promoting <i>Streptomyces misionensis</i> SwB1 Microorganisms phosphate solubilizer phosphorus mobilization mechanism comparative genomics <i>Cornus wilsoniana</i> microbial fertilizer |
| title | Revealing the Existence of Diverse Strategies for Phosphorus Solubilization and Acquisition in Plant-Growth Promoting <i>Streptomyces misionensis</i> SwB1 |
| title_full | Revealing the Existence of Diverse Strategies for Phosphorus Solubilization and Acquisition in Plant-Growth Promoting <i>Streptomyces misionensis</i> SwB1 |
| title_fullStr | Revealing the Existence of Diverse Strategies for Phosphorus Solubilization and Acquisition in Plant-Growth Promoting <i>Streptomyces misionensis</i> SwB1 |
| title_full_unstemmed | Revealing the Existence of Diverse Strategies for Phosphorus Solubilization and Acquisition in Plant-Growth Promoting <i>Streptomyces misionensis</i> SwB1 |
| title_short | Revealing the Existence of Diverse Strategies for Phosphorus Solubilization and Acquisition in Plant-Growth Promoting <i>Streptomyces misionensis</i> SwB1 |
| title_sort | revealing the existence of diverse strategies for phosphorus solubilization and acquisition in plant growth promoting i streptomyces misionensis i swb1 |
| topic | phosphate solubilizer phosphorus mobilization mechanism comparative genomics <i>Cornus wilsoniana</i> microbial fertilizer |
| url | https://www.mdpi.com/2076-2607/13/2/378 |
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