Polyallylamine Hydrochloride-Modified Bovine Serum Albumin Nanoparticles Loaded with α-Solanine for Chemotherapy of Pancreatic Cancer
Zhengde Wen,1,2,* Shan Luo,2,3,* Juntao Liu,4 Yufan Huang,4 Gang Chen,1 Huajie Cai1 1Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China; 2Wenzhou Key Laboratory of Perioperative Medicine, The First Affilia...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2025-04-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/polyallylamine-hydrochloride-modified-bovine-serum-albumin-nanoparticl-peer-reviewed-fulltext-article-IJN |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849702129421254656 |
|---|---|
| author | Wen Z Luo S Liu J Huang Y Chen G Cai H |
| author_facet | Wen Z Luo S Liu J Huang Y Chen G Cai H |
| author_sort | Wen Z |
| collection | DOAJ |
| description | Zhengde Wen,1,2,* Shan Luo,2,3,* Juntao Liu,4 Yufan Huang,4 Gang Chen,1 Huajie Cai1 1Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China; 2Wenzhou Key Laboratory of Perioperative Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China; 3Department of Anaesthesia, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China; 4Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Huajie Cai; Gang Chen, Email chjwzmu@163.com; qwechen1119@163.comIntroduction: α-Solanine (α-Sol) shows promise for pancreatic cancer (PC) treatment by inhibiting PC cell proliferation, migration, and invasion. However, its clinical application is hindered by poor tumor targeting, significant toxicity, and undesirable pharmacokinetics. To address these issues, this study developed a nanoparticle delivery system (PBSO NPs) using bovine serum albumin as a carrier, with polyallylamine hydrochloride surface modification to enhance α-Sol delivery.Methods: PBSO NPs were characterized using transmission electron microscopy, dynamic light scattering, nanoparticle size analyzers, and Fourier-transform infrared spectroscopy. Their in vitro drug release profile and cellular uptake capabilities were evaluated. Furthermore, in vitro experiments were conducted using mouse pancreatic cancer cells (Panc02) to investigate the effects of PBSO NPs on Panc02 cell viability, migration, invasion, and apoptosis. Additionally, a pancreatic cancer xenograft tumor model was established for in vivo experiments to explore the impact of PBSO NPs on tumor growth.Results: This study successfully developed PBSO NPs with favorable morphology and physiological stability, capable of enhancing cellular uptake. In vitro experiments demonstrated that PBSO NPs significantly inhibited the viability, migration, and invasion of Panc02 cells while promoting apoptosis. Moreover, PBSO NPs enhanced the inhibitory effects of α-Sol on Panc02 cells. In vivo experiments further confirmed that PBSO NPs improved the therapeutic efficacy of α-Sol against PC while partially reducing its toxicity. Additionally, PBSO NPs exhibited good biocompatibility.Discussion: PBSO NPs enhance the therapeutic efficacy of α-Sol against PC by inhibiting the viability, migration, and invasion of PC cells while promoting apoptosis, thereby suppressing the progression of PC. This provides a promising therapeutic strategy for pancreatic cancer treatment.Keywords: pancreatic cancer, α-solanine, bovine serum albumin, polyallylamine hydrochloride, apoptosis |
| format | Article |
| id | doaj-art-c1f6bf2cd164443092b3b0a8d42f067e |
| institution | DOAJ |
| issn | 1178-2013 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj-art-c1f6bf2cd164443092b3b0a8d42f067e2025-08-20T03:17:44ZengDove Medical PressInternational Journal of Nanomedicine1178-20132025-04-01Volume 2042354255101849Polyallylamine Hydrochloride-Modified Bovine Serum Albumin Nanoparticles Loaded with α-Solanine for Chemotherapy of Pancreatic CancerWen ZLuo SLiu JHuang YChen GCai HZhengde Wen,1,2,* Shan Luo,2,3,* Juntao Liu,4 Yufan Huang,4 Gang Chen,1 Huajie Cai1 1Department of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China; 2Wenzhou Key Laboratory of Perioperative Medicine, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China; 3Department of Anaesthesia, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China; 4Wenzhou Medical University, Wenzhou, Zhejiang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Huajie Cai; Gang Chen, Email chjwzmu@163.com; qwechen1119@163.comIntroduction: α-Solanine (α-Sol) shows promise for pancreatic cancer (PC) treatment by inhibiting PC cell proliferation, migration, and invasion. However, its clinical application is hindered by poor tumor targeting, significant toxicity, and undesirable pharmacokinetics. To address these issues, this study developed a nanoparticle delivery system (PBSO NPs) using bovine serum albumin as a carrier, with polyallylamine hydrochloride surface modification to enhance α-Sol delivery.Methods: PBSO NPs were characterized using transmission electron microscopy, dynamic light scattering, nanoparticle size analyzers, and Fourier-transform infrared spectroscopy. Their in vitro drug release profile and cellular uptake capabilities were evaluated. Furthermore, in vitro experiments were conducted using mouse pancreatic cancer cells (Panc02) to investigate the effects of PBSO NPs on Panc02 cell viability, migration, invasion, and apoptosis. Additionally, a pancreatic cancer xenograft tumor model was established for in vivo experiments to explore the impact of PBSO NPs on tumor growth.Results: This study successfully developed PBSO NPs with favorable morphology and physiological stability, capable of enhancing cellular uptake. In vitro experiments demonstrated that PBSO NPs significantly inhibited the viability, migration, and invasion of Panc02 cells while promoting apoptosis. Moreover, PBSO NPs enhanced the inhibitory effects of α-Sol on Panc02 cells. In vivo experiments further confirmed that PBSO NPs improved the therapeutic efficacy of α-Sol against PC while partially reducing its toxicity. Additionally, PBSO NPs exhibited good biocompatibility.Discussion: PBSO NPs enhance the therapeutic efficacy of α-Sol against PC by inhibiting the viability, migration, and invasion of PC cells while promoting apoptosis, thereby suppressing the progression of PC. This provides a promising therapeutic strategy for pancreatic cancer treatment.Keywords: pancreatic cancer, α-solanine, bovine serum albumin, polyallylamine hydrochloride, apoptosishttps://www.dovepress.com/polyallylamine-hydrochloride-modified-bovine-serum-albumin-nanoparticl-peer-reviewed-fulltext-article-IJNpancreatic cancerα-solaninebovine serum albuminpolyallylamine hydrochlorideapoptosis |
| spellingShingle | Wen Z Luo S Liu J Huang Y Chen G Cai H Polyallylamine Hydrochloride-Modified Bovine Serum Albumin Nanoparticles Loaded with α-Solanine for Chemotherapy of Pancreatic Cancer International Journal of Nanomedicine pancreatic cancer α-solanine bovine serum albumin polyallylamine hydrochloride apoptosis |
| title | Polyallylamine Hydrochloride-Modified Bovine Serum Albumin Nanoparticles Loaded with α-Solanine for Chemotherapy of Pancreatic Cancer |
| title_full | Polyallylamine Hydrochloride-Modified Bovine Serum Albumin Nanoparticles Loaded with α-Solanine for Chemotherapy of Pancreatic Cancer |
| title_fullStr | Polyallylamine Hydrochloride-Modified Bovine Serum Albumin Nanoparticles Loaded with α-Solanine for Chemotherapy of Pancreatic Cancer |
| title_full_unstemmed | Polyallylamine Hydrochloride-Modified Bovine Serum Albumin Nanoparticles Loaded with α-Solanine for Chemotherapy of Pancreatic Cancer |
| title_short | Polyallylamine Hydrochloride-Modified Bovine Serum Albumin Nanoparticles Loaded with α-Solanine for Chemotherapy of Pancreatic Cancer |
| title_sort | polyallylamine hydrochloride modified bovine serum albumin nanoparticles loaded with alpha solanine for chemotherapy of pancreatic cancer |
| topic | pancreatic cancer α-solanine bovine serum albumin polyallylamine hydrochloride apoptosis |
| url | https://www.dovepress.com/polyallylamine-hydrochloride-modified-bovine-serum-albumin-nanoparticl-peer-reviewed-fulltext-article-IJN |
| work_keys_str_mv | AT wenz polyallylaminehydrochloridemodifiedbovineserumalbuminnanoparticlesloadedwithalphasolanineforchemotherapyofpancreaticcancer AT luos polyallylaminehydrochloridemodifiedbovineserumalbuminnanoparticlesloadedwithalphasolanineforchemotherapyofpancreaticcancer AT liuj polyallylaminehydrochloridemodifiedbovineserumalbuminnanoparticlesloadedwithalphasolanineforchemotherapyofpancreaticcancer AT huangy polyallylaminehydrochloridemodifiedbovineserumalbuminnanoparticlesloadedwithalphasolanineforchemotherapyofpancreaticcancer AT cheng polyallylaminehydrochloridemodifiedbovineserumalbuminnanoparticlesloadedwithalphasolanineforchemotherapyofpancreaticcancer AT caih polyallylaminehydrochloridemodifiedbovineserumalbuminnanoparticlesloadedwithalphasolanineforchemotherapyofpancreaticcancer |