L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway
Rat studies demonstrated that type II diabetes mellitus (T2DM) decreases both the production and bioavailability of nitric oxide (NO). L-arginine (LA) provides the precursor for the production of NO. We hypothesized that LA dietary supplementation will preserve NO production via endothelial nitric o...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/171546 |
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| author | Taylor Claybaugh Sarah Decker Kelly McCall Yuriy Slyvka Jerrod Steimle Aaron Wood Megan Schaefer Jean Thuma Sharon Inman |
| author_facet | Taylor Claybaugh Sarah Decker Kelly McCall Yuriy Slyvka Jerrod Steimle Aaron Wood Megan Schaefer Jean Thuma Sharon Inman |
| author_sort | Taylor Claybaugh |
| collection | DOAJ |
| description | Rat studies demonstrated that type II diabetes mellitus (T2DM) decreases both the production and bioavailability of nitric oxide (NO). L-arginine (LA) provides the precursor for the production of NO. We hypothesized that LA dietary supplementation will preserve NO production via endothelial nitric oxide synthase (eNOS) causing renal microvascular vasodilation and increased glomerular blood flow and thus increasing glomerular filtration rate (GFR). This would impede the formation of reactive oxygen species which contributes to cell damage and death. LA supplementation preserved GFR in the treated diabetic rats compared to untreated diabetic rats. We provide evidence that this effect may be due to increased levels of eNOS and urinary cyclic guanosine monophosphate, which leads to renal microvascular vasodilation. Plasma nitrotyrosine was decreased in the LA treated rats; however, plasma nitrite levels remained unaffected as expected. Marked improvements in glucose tolerance were also observed in the LA treated diabetic rats. These results demonstrate that LA supplementation preserves NO activity and may delay the onset of insulin resistance and renal dysfunction during hyperglycemic stress. These results suggest the importance of the NO pathway in consequent renal dysfunction and in the development of insulin resistance in diabetic rats. |
| format | Article |
| id | doaj-art-c1e8b6c1a7494f3cabf48f3c5bd76d05 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-c1e8b6c1a7494f3cabf48f3c5bd76d052025-02-03T01:31:24ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/171546171546L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide PathwayTaylor Claybaugh0Sarah Decker1Kelly McCall2Yuriy Slyvka3Jerrod Steimle4Aaron Wood5Megan Schaefer6Jean Thuma7Sharon Inman8Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, 228 Irvine, Athens, OH 45701, USADepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, 228 Irvine, Athens, OH 45701, USADepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, 228 Irvine, Athens, OH 45701, USADepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, 228 Irvine, Athens, OH 45701, USADepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, 228 Irvine, Athens, OH 45701, USADepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, 228 Irvine, Athens, OH 45701, USADepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, 228 Irvine, Athens, OH 45701, USAThe Diabetes Institute at Ohio University, Ohio University, 228 Irvine, Athens, OH 45701, USADepartment of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, 228 Irvine, Athens, OH 45701, USARat studies demonstrated that type II diabetes mellitus (T2DM) decreases both the production and bioavailability of nitric oxide (NO). L-arginine (LA) provides the precursor for the production of NO. We hypothesized that LA dietary supplementation will preserve NO production via endothelial nitric oxide synthase (eNOS) causing renal microvascular vasodilation and increased glomerular blood flow and thus increasing glomerular filtration rate (GFR). This would impede the formation of reactive oxygen species which contributes to cell damage and death. LA supplementation preserved GFR in the treated diabetic rats compared to untreated diabetic rats. We provide evidence that this effect may be due to increased levels of eNOS and urinary cyclic guanosine monophosphate, which leads to renal microvascular vasodilation. Plasma nitrotyrosine was decreased in the LA treated rats; however, plasma nitrite levels remained unaffected as expected. Marked improvements in glucose tolerance were also observed in the LA treated diabetic rats. These results demonstrate that LA supplementation preserves NO activity and may delay the onset of insulin resistance and renal dysfunction during hyperglycemic stress. These results suggest the importance of the NO pathway in consequent renal dysfunction and in the development of insulin resistance in diabetic rats.http://dx.doi.org/10.1155/2014/171546 |
| spellingShingle | Taylor Claybaugh Sarah Decker Kelly McCall Yuriy Slyvka Jerrod Steimle Aaron Wood Megan Schaefer Jean Thuma Sharon Inman L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway International Journal of Endocrinology |
| title | L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway |
| title_full | L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway |
| title_fullStr | L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway |
| title_full_unstemmed | L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway |
| title_short | L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway |
| title_sort | l arginine supplementation in type ii diabetic rats preserves renal function and improves insulin sensitivity by altering the nitric oxide pathway |
| url | http://dx.doi.org/10.1155/2014/171546 |
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