Medulla Tetrapanacis water extract ameliorates mastitis by suppressing bacterial internalization and inflammation via MAPKs signaling in vitro and in vivo
Abstract Medulla Tetrapanacis (MT) is a commonly used herbal ingredient in soup to promote lactation and management of mastitis among lactating mothers worldwide, particularly in Asian countries. However, scientific evidence to support its usage in the management of mastitis is limited. This study a...
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| Format: | Article |
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Wiley
2025-01-01
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| Series: | Food Frontiers |
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| Online Access: | https://doi.org/10.1002/fft2.476 |
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| author | Carsten Tsun‐Ka Kwok Yuanhao Hu Bun Tsoi Fiona Wong Pak‐Ting Hau Emily Wan‐Ting Tam Daniel Kam‐Wah Mok Yiu‐Wa Kwan George Pak‐Heng Leung Simon Ming‐Yuen Lee Jing‐Jing Li Franklin Wang‐Ngai Chow Sai‐Wang Seto |
| author_facet | Carsten Tsun‐Ka Kwok Yuanhao Hu Bun Tsoi Fiona Wong Pak‐Ting Hau Emily Wan‐Ting Tam Daniel Kam‐Wah Mok Yiu‐Wa Kwan George Pak‐Heng Leung Simon Ming‐Yuen Lee Jing‐Jing Li Franklin Wang‐Ngai Chow Sai‐Wang Seto |
| author_sort | Carsten Tsun‐Ka Kwok |
| collection | DOAJ |
| description | Abstract Medulla Tetrapanacis (MT) is a commonly used herbal ingredient in soup to promote lactation and management of mastitis among lactating mothers worldwide, particularly in Asian countries. However, scientific evidence to support its usage in the management of mastitis is limited. This study aimed to investigate the effects of MT water extract and its underlying mechanisms in Staphylococcus aureus (SA)–induced mastitis in human mammary epithelial cells (HuMEC) and lipopolysaccharide (LPS)–induced mastitis in lactating Sprague–Dawley (SD) rats. The anti‐inflammatory and antibacterial effects of MT water extract were examined in SA‐infected HuMEC by ELISA and plate counting, respectively. The effects of MT water extract on blood‐milk barrier and the underlying mechanism of action of the MT water extract were also investigated by transendothelial electrical resistance assay and western blot. The effects and mechanisms of MT water extract on alleviating mastitis on SD rats were evaluated using LPS‐induced mastitis rat model. Our results showed that MT water extract could suppress SA‐induced mastitis by reducing SA internalization and growth, protecting blood‐milk barrier integrity, and attenuating release of cytokines (interleukin 6 [IL‐6] and tumor necrosis factor‐alpha [TNF‐α]) in HuEMC. Furthermore, the antibacterial effect might be related to the increase of antimicrobial peptides transcription, and the anti‐inflammatory effect is at least partly mediated by inactivation of p38 and JNK signaling pathways. Finally, our in vivo results showed that MT water extract ameliorated LPS‐induced mastitis in SD rats via suppressing inflammatory cytokine release (TNF‐α, IL‐6, and interleukin‐1 beta), myeloperoxidase, and alleviating pathohistological damage by downregulation of JNK signaling pathway. |
| format | Article |
| id | doaj-art-c1e819c08096437d83c13cfecc7cdb36 |
| institution | OA Journals |
| issn | 2643-8429 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Frontiers |
| spelling | doaj-art-c1e819c08096437d83c13cfecc7cdb362025-08-20T01:55:22ZengWileyFood Frontiers2643-84292025-01-016150051510.1002/fft2.476Medulla Tetrapanacis water extract ameliorates mastitis by suppressing bacterial internalization and inflammation via MAPKs signaling in vitro and in vivoCarsten Tsun‐Ka Kwok0Yuanhao Hu1Bun Tsoi2Fiona Wong3Pak‐Ting Hau4Emily Wan‐Ting Tam5Daniel Kam‐Wah Mok6Yiu‐Wa Kwan7George Pak‐Heng Leung8Simon Ming‐Yuen Lee9Jing‐Jing Li10Franklin Wang‐Ngai Chow11Sai‐Wang Seto12Department of Food Science and Nutrition The Hong Kong Polytechnic University Hong Kong ChinaDepartment of Food Science and Nutrition The Hong Kong Polytechnic University Hong Kong ChinaDepartment of Food Science and Nutrition The Hong Kong Polytechnic University Hong Kong ChinaDepartment of Food Science and Nutrition The Hong Kong Polytechnic University Hong Kong ChinaDepartment of Health Technology and Informatics The Hong Kong Polytechnic University Hong Kong ChinaDepartment of Applied Sciences, Faculty of Science and Technology Hong Kong Metropolitan University Hong Kong ChinaDepartment of Food Science and Nutrition The Hong Kong Polytechnic University Hong Kong ChinaSchool of Biomedical Sciences The Chinese University of Hong Kong Hong Kong ChinaDepartment of Pharmacology and Pharmacy The University of Hong Kong Hong Kong ChinaDepartment of Food Science and Nutrition The Hong Kong Polytechnic University Hong Kong ChinaResearch Centre for Chinese Medicine Innovation The Hong Kong Polytechnic University Hong Kong ChinaResearch Centre for Chinese Medicine Innovation The Hong Kong Polytechnic University Hong Kong ChinaDepartment of Food Science and Nutrition The Hong Kong Polytechnic University Hong Kong ChinaAbstract Medulla Tetrapanacis (MT) is a commonly used herbal ingredient in soup to promote lactation and management of mastitis among lactating mothers worldwide, particularly in Asian countries. However, scientific evidence to support its usage in the management of mastitis is limited. This study aimed to investigate the effects of MT water extract and its underlying mechanisms in Staphylococcus aureus (SA)–induced mastitis in human mammary epithelial cells (HuMEC) and lipopolysaccharide (LPS)–induced mastitis in lactating Sprague–Dawley (SD) rats. The anti‐inflammatory and antibacterial effects of MT water extract were examined in SA‐infected HuMEC by ELISA and plate counting, respectively. The effects of MT water extract on blood‐milk barrier and the underlying mechanism of action of the MT water extract were also investigated by transendothelial electrical resistance assay and western blot. The effects and mechanisms of MT water extract on alleviating mastitis on SD rats were evaluated using LPS‐induced mastitis rat model. Our results showed that MT water extract could suppress SA‐induced mastitis by reducing SA internalization and growth, protecting blood‐milk barrier integrity, and attenuating release of cytokines (interleukin 6 [IL‐6] and tumor necrosis factor‐alpha [TNF‐α]) in HuEMC. Furthermore, the antibacterial effect might be related to the increase of antimicrobial peptides transcription, and the anti‐inflammatory effect is at least partly mediated by inactivation of p38 and JNK signaling pathways. Finally, our in vivo results showed that MT water extract ameliorated LPS‐induced mastitis in SD rats via suppressing inflammatory cytokine release (TNF‐α, IL‐6, and interleukin‐1 beta), myeloperoxidase, and alleviating pathohistological damage by downregulation of JNK signaling pathway.https://doi.org/10.1002/fft2.476antimicrobial peptideblood–milk barrierinflammationMAPK‐signaling pathwaymastitisMedulla Tetrapanacis |
| spellingShingle | Carsten Tsun‐Ka Kwok Yuanhao Hu Bun Tsoi Fiona Wong Pak‐Ting Hau Emily Wan‐Ting Tam Daniel Kam‐Wah Mok Yiu‐Wa Kwan George Pak‐Heng Leung Simon Ming‐Yuen Lee Jing‐Jing Li Franklin Wang‐Ngai Chow Sai‐Wang Seto Medulla Tetrapanacis water extract ameliorates mastitis by suppressing bacterial internalization and inflammation via MAPKs signaling in vitro and in vivo Food Frontiers antimicrobial peptide blood–milk barrier inflammation MAPK‐signaling pathway mastitis Medulla Tetrapanacis |
| title | Medulla Tetrapanacis water extract ameliorates mastitis by suppressing bacterial internalization and inflammation via MAPKs signaling in vitro and in vivo |
| title_full | Medulla Tetrapanacis water extract ameliorates mastitis by suppressing bacterial internalization and inflammation via MAPKs signaling in vitro and in vivo |
| title_fullStr | Medulla Tetrapanacis water extract ameliorates mastitis by suppressing bacterial internalization and inflammation via MAPKs signaling in vitro and in vivo |
| title_full_unstemmed | Medulla Tetrapanacis water extract ameliorates mastitis by suppressing bacterial internalization and inflammation via MAPKs signaling in vitro and in vivo |
| title_short | Medulla Tetrapanacis water extract ameliorates mastitis by suppressing bacterial internalization and inflammation via MAPKs signaling in vitro and in vivo |
| title_sort | medulla tetrapanacis water extract ameliorates mastitis by suppressing bacterial internalization and inflammation via mapks signaling in vitro and in vivo |
| topic | antimicrobial peptide blood–milk barrier inflammation MAPK‐signaling pathway mastitis Medulla Tetrapanacis |
| url | https://doi.org/10.1002/fft2.476 |
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