Mapping and identification of genes responsible for less-photosensitive fruit coloration in eggplant

In eggplant (Solanum melongena. L), low light during cultivation often hinders proper pigmentation of fruit. While some varieties exhibit less susceptibility to low light for eggplant coloration, however, the genetic basis of such less-photosensitive fruit coloration remains unknown. In this study,...

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Main Authors: Lei Luo, Yinan Niu, Qiang Li, Linfeng Xia, Chunyang Wang, Shuangxia Luo, Na Li, Shuxin Xuan, Yanhua Wang, Shuxing Shen, Jianjun Zhao, Xueping Chen
Format: Article
Language:English
Published: Maximum Academic Press 2023-01-01
Series:Vegetable Research
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Online Access:https://www.maxapress.com/article/doi/10.48130/VR-2023-0032
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Summary:In eggplant (Solanum melongena. L), low light during cultivation often hinders proper pigmentation of fruit. While some varieties exhibit less susceptibility to low light for eggplant coloration, however, the genetic basis of such less-photosensitive fruit coloration remains unknown. In this study, we characterized a less-photosensitive eggplant cultivar '609'. Under bagging conditions, fruits of '609' exhibited purple coloration, albeit lighter than fruits grown under natural conditions. Genetic analysis showed that the less-photosensitive trait was controlled by a single dominant gene, designated SmLP. Based on BSA and genetic recombination analyses, SmLP was mapped to the 7.4−12.5 Mb region on chromosome 10. Within this genetic region, six genes with non-synonymous mutation and seven genes potentially involved in anthocyanin biosynthesis or light signal transduction were identified. Further RT-qPCR analysis revealed that only three out of these genes were differentially expressed in eggplant peel tissues. The three genes EGP21875, EGP21864 and EGP21911, encoding MYB domain protein 113, phototropic-responsive NPH3 family protein, and protein with unknown function, respectively, were considered as the putative genes associated with less-photosensitive anthocyanin biosynthesis in eggplant fruits. These results would provide great help in casual gene identification for less-photosensitive trait and promote an understanding of molecular mechanisms underlying less- and non-photosensitive coloration in eggplant.
ISSN:2769-0520