Moderate-intensity training has a better effect on growth factors than high-intensity training in rats
Introduction: Exercise is widely known to be a powerful stimulus to the endocrine system. Several previous studies have examined the effects of exercise on GH, melatonin, or cortisol separately. However, to date, no study has directly examined all three hormones simultaneously within a single exerc...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
FEADEF
2025-06-01
|
| Series: | Retos: Nuevas Tendencias en Educación Física, Deportes y Recreación |
| Subjects: | |
| Online Access: | https://revistaretos.org/index.php/retos/article/view/116181 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849691776769589248 |
|---|---|
| author | Laily Mita Andriana Adi Pranoto Greta Ahmad Bukhori Joseph Kenoly Nugroho Irmantara Subagio Nining Widyah Kusnanik Bayu Agung Pramono Dany Pramuno Putra Muhamad Fauzi Antoni Bekir Erhan Orhan |
| author_facet | Laily Mita Andriana Adi Pranoto Greta Ahmad Bukhori Joseph Kenoly Nugroho Irmantara Subagio Nining Widyah Kusnanik Bayu Agung Pramono Dany Pramuno Putra Muhamad Fauzi Antoni Bekir Erhan Orhan |
| author_sort | Laily Mita Andriana |
| collection | DOAJ |
| description |
Introduction: Exercise is widely known to be a powerful stimulus to the endocrine system. Several previous studies have examined the effects of exercise on GH, melatonin, or cortisol separately. However, to date, no study has directly examined all three hormones simultaneously within a single exercise protocol, especially using a controlled rat models.
Objective: The study aims to determine the effect of moderate-intensity training and high-intensity training on growth determinants in rats.
Methodology: A total of 39 male rats, Rattus Norvegicus strain Wistar type, 8 weeks aged, 160±20 gram and randomly divided into three groups: CON (n = 13, controls without treatment), MIT (n = 13, moderate-intensity training, using a treadmill with a speed of 14-16 m/min for 30 minutes and HIT (n = 13, high-intensity training, using a treadmill at a speed of 22-25 m/min during 20 minutes). Treatment was carried out from 17.00-21.00 p.m. with a frequency of 3 times/week for 12 weeks. Blood was taken after 24 hours after the last exercise treatment. Serum measurements of growth hormone (GH), melatonin and cortisol using the ELISA. The data were analyzed by using one way-ANOVA and Tukey’s HSD post hoc test with significant levels of 5%.
Results: We observed GH levels in CON (4.30 ± 0.29) pg/mL, MIT (4.55 ± 0.17) pg/mL, HIT (4.09 ± 0.28) pg/mL and (p < 0.001). Melatonin levels in CON (172.31 ± 15.86) pg/mL, MIT (193.54 ± 25.01) pg/mL, HIT (165.96 ± 15.44) pg/mL and (p < 0.05). Cortisol levels in CON (247.22 ± 50.26) ng/mL, MIT (212.82 ± 41.24) ng/mL, HIT (262.11 ± 19.56) ng/mL and (p < 0.05).
Conclusions: These findings suggest that moderate-intensity training has a better effect on growth determinants compared to high-intensity training in rats.
|
| format | Article |
| id | doaj-art-c1df5281c0ef462f812ff7d2bca8a22d |
| institution | DOAJ |
| issn | 1579-1726 1988-2041 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | FEADEF |
| record_format | Article |
| series | Retos: Nuevas Tendencias en Educación Física, Deportes y Recreación |
| spelling | doaj-art-c1df5281c0ef462f812ff7d2bca8a22d2025-08-20T03:20:55ZengFEADEFRetos: Nuevas Tendencias en Educación Física, Deportes y Recreación1579-17261988-20412025-06-016810.47197/retos.v68.116181Moderate-intensity training has a better effect on growth factors than high-intensity training in ratsLaily Mita Andriana0https://orcid.org/0009-0002-9845-6577Adi Pranoto1https://orcid.org/0000-0003-4080-9245Greta Ahmad Bukhori2Joseph Kenoly Nugroho3Irmantara Subagio4Nining Widyah Kusnanik5Bayu Agung Pramono6Dany Pramuno Putra7https://orcid.org/0009-0009-2650-9810Muhamad Fauzi Antoni8https://orcid.org/0009-0001-3482-3297Bekir Erhan Orhan9Department of Sport Coaching Education, Faculty of Sport and Health Sciences, Universitas Negeri SurabayaDepartment of Sports Coaching Education, Faculty of Sports and Health Science, Universitas Negeri SurabayaAtlas Sports Club MalangDepartment of Sport Science, Faculty of Sport Science, Universitas Negeri MalangDepartment of Sport Coaching Education, Faculty of Sport and Health Sciences, Universitas Negeri SurabayaDepartment of Sport Coaching Education, Faculty of Sport and Health Sciences, Universitas Negeri SurabayaDepartment of Sport Coaching Education, Faculty of Sport and Health Sciences, Universitas Negeri SurabayaFaculty of Vocational, Universitas AirlanggaDepartment of Sport Coaching Education, Faculty of Sport and Health Sciences, Universitas Negeri SurabayaFaculty of Sports Sciences, Istanbul Aydın University Introduction: Exercise is widely known to be a powerful stimulus to the endocrine system. Several previous studies have examined the effects of exercise on GH, melatonin, or cortisol separately. However, to date, no study has directly examined all three hormones simultaneously within a single exercise protocol, especially using a controlled rat models. Objective: The study aims to determine the effect of moderate-intensity training and high-intensity training on growth determinants in rats. Methodology: A total of 39 male rats, Rattus Norvegicus strain Wistar type, 8 weeks aged, 160±20 gram and randomly divided into three groups: CON (n = 13, controls without treatment), MIT (n = 13, moderate-intensity training, using a treadmill with a speed of 14-16 m/min for 30 minutes and HIT (n = 13, high-intensity training, using a treadmill at a speed of 22-25 m/min during 20 minutes). Treatment was carried out from 17.00-21.00 p.m. with a frequency of 3 times/week for 12 weeks. Blood was taken after 24 hours after the last exercise treatment. Serum measurements of growth hormone (GH), melatonin and cortisol using the ELISA. The data were analyzed by using one way-ANOVA and Tukey’s HSD post hoc test with significant levels of 5%. Results: We observed GH levels in CON (4.30 ± 0.29) pg/mL, MIT (4.55 ± 0.17) pg/mL, HIT (4.09 ± 0.28) pg/mL and (p < 0.001). Melatonin levels in CON (172.31 ± 15.86) pg/mL, MIT (193.54 ± 25.01) pg/mL, HIT (165.96 ± 15.44) pg/mL and (p < 0.05). Cortisol levels in CON (247.22 ± 50.26) ng/mL, MIT (212.82 ± 41.24) ng/mL, HIT (262.11 ± 19.56) ng/mL and (p < 0.05). Conclusions: These findings suggest that moderate-intensity training has a better effect on growth determinants compared to high-intensity training in rats. https://revistaretos.org/index.php/retos/article/view/116181Cortisolgrowth hormonemelatoninmoderate-intensity traininghigh-intensity training |
| spellingShingle | Laily Mita Andriana Adi Pranoto Greta Ahmad Bukhori Joseph Kenoly Nugroho Irmantara Subagio Nining Widyah Kusnanik Bayu Agung Pramono Dany Pramuno Putra Muhamad Fauzi Antoni Bekir Erhan Orhan Moderate-intensity training has a better effect on growth factors than high-intensity training in rats Retos: Nuevas Tendencias en Educación Física, Deportes y Recreación Cortisol growth hormone melatonin moderate-intensity training high-intensity training |
| title | Moderate-intensity training has a better effect on growth factors than high-intensity training in rats |
| title_full | Moderate-intensity training has a better effect on growth factors than high-intensity training in rats |
| title_fullStr | Moderate-intensity training has a better effect on growth factors than high-intensity training in rats |
| title_full_unstemmed | Moderate-intensity training has a better effect on growth factors than high-intensity training in rats |
| title_short | Moderate-intensity training has a better effect on growth factors than high-intensity training in rats |
| title_sort | moderate intensity training has a better effect on growth factors than high intensity training in rats |
| topic | Cortisol growth hormone melatonin moderate-intensity training high-intensity training |
| url | https://revistaretos.org/index.php/retos/article/view/116181 |
| work_keys_str_mv | AT lailymitaandriana moderateintensitytraininghasabettereffectongrowthfactorsthanhighintensitytraininginrats AT adipranoto moderateintensitytraininghasabettereffectongrowthfactorsthanhighintensitytraininginrats AT gretaahmadbukhori moderateintensitytraininghasabettereffectongrowthfactorsthanhighintensitytraininginrats AT josephkenolynugroho moderateintensitytraininghasabettereffectongrowthfactorsthanhighintensitytraininginrats AT irmantarasubagio moderateintensitytraininghasabettereffectongrowthfactorsthanhighintensitytraininginrats AT niningwidyahkusnanik moderateintensitytraininghasabettereffectongrowthfactorsthanhighintensitytraininginrats AT bayuagungpramono moderateintensitytraininghasabettereffectongrowthfactorsthanhighintensitytraininginrats AT danypramunoputra moderateintensitytraininghasabettereffectongrowthfactorsthanhighintensitytraininginrats AT muhamadfauziantoni moderateintensitytraininghasabettereffectongrowthfactorsthanhighintensitytraininginrats AT bekirerhanorhan moderateintensitytraininghasabettereffectongrowthfactorsthanhighintensitytraininginrats |