Reduced folate carrier 1 (<i>RFC1</i>) gene polymorphisms among acute lymphoblastic leukemia patients
Reduced Folate Carrier1 (RFC1) gene's metabolism is crucial for DNA synthesis, epigenetic mechanisms, and cellular methylation events. Nonetheless, RFC1 polymorphisms have drawn a lot of interest in current medical genetics studies. The objectives of the study were to ascertain the relationship...
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PAGEPress Publications
2025-03-01
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| Series: | Journal of Biological Research |
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| Online Access: | https://www.pagepressjournals.org/jbr/article/view/13105 |
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| author | Mashaer Taha Edris Maye Mohammad Merghani Shaza Salih Gafar Ahmed Mohamed Asmeli Elrashed B. Yasin Raed Alserihi Haitham M.H. Qutob Aymen Yasin Elshazali Ali |
| author_facet | Mashaer Taha Edris Maye Mohammad Merghani Shaza Salih Gafar Ahmed Mohamed Asmeli Elrashed B. Yasin Raed Alserihi Haitham M.H. Qutob Aymen Yasin Elshazali Ali |
| author_sort | Mashaer Taha Edris |
| collection | DOAJ |
| description | Reduced Folate Carrier1 (RFC1) gene's metabolism is crucial for DNA synthesis, epigenetic mechanisms, and cellular methylation events. Nonetheless, RFC1 polymorphisms have drawn a lot of interest in current medical genetics studies. The objectives of the study were to ascertain the relationship between the risk of acute lymphoblastic leukemia in Sudanese patients and the genetic variant of RFC1 (G 80A). Using Real-Time Polymerase Chain Reaction (RT-PCR) method, 150 Acute Lymphoblastic Leukemia (ALL) patients and 150 healthy control volunteers had their DNA collected and examined for the case-control study. Version 25 of the Statistical Package for Social Science (SPSS) software was used to analyze the data. There was no discernible difference in the age or gender of the patients compared to the controls. According to Flow cytometry data, T-ALL accounts for 31 (21%) and B cell type accounts for 119 (79%). According to this study, there is a statistically significant correlation between the mean blast (p=0.050) and the mean total white blood cell count (p=0.000) in T-ALL cases. The genotype frequency of RFC1 (G80A) was found to be GG 39 (26.0%), GA 41 (27.3%), and 70 (46.7%) for AA in the cases, while GG 41 (27.3%), GA 28 (18.7%), and AA 81 (54.0%) in the control group. While the homozygous genotypes AA and GG were more common in the control group, the heterozygous genotype GA was more common in the patient group. On the other hand, the genotype distribution among the research groups was not statistically significant with odds ratio: 0.745 (0.473-1.174), p=0.192. In conclusion, RFC1 (G80A) polymorphism was not found to be associated with an increased risk of ALL, according to the findings of this investigation.
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| format | Article |
| id | doaj-art-c1deeae32f00469e8eef68c71b7659d2 |
| institution | DOAJ |
| issn | 1826-8838 2284-0230 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | PAGEPress Publications |
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| series | Journal of Biological Research |
| spelling | doaj-art-c1deeae32f00469e8eef68c71b7659d22025-08-20T02:49:40ZengPAGEPress PublicationsJournal of Biological Research1826-88382284-02302025-03-0110.4081/jbr.2025.13105Reduced folate carrier 1 (<i>RFC1</i>) gene polymorphisms among acute lymphoblastic leukemia patientsMashaer Taha Edris0Maye Mohammad Merghani 1Shaza Salih Gafar 2Ahmed Mohamed Asmeli 3Elrashed B. Yasin4Raed Alserihi 5Haitham M.H. Qutob 6Aymen Yasin7Elshazali Ali8Department of Haematology, Faculty of Medical Laboratory Sciences, National Ribat University, Khartoum, Sudan; Department of Haematology, Faculty of Medical Laboratory Sciences, Nahda College, KhartoumDepartment of Haematology, Faculty of Medical Laboratory Sciences, Nahda College, KhartoumDepartment of Microbiology, Faculty of Medical Laboratory Sciences, Nahda College, KhartoumDepartment of Clinical Chemistry, Faculty of Medical Laboratory Sciences, Nahda College, KhartoumDepartment of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, RabighDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Hematology Research Unit (HRU), King Fahad Medical Research Center (KFMRC), King Abdulaziz University, JeddahDepartment of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, RabighAccident and Emergency Department, Shrewsbury and Telford NHS Trust, The Royal Shrewsbury Hospital, ShrewsburyDepartment of Haematology, Faculty of Medical Laboratory Sciences, Al Neelain University, KhartoumReduced Folate Carrier1 (RFC1) gene's metabolism is crucial for DNA synthesis, epigenetic mechanisms, and cellular methylation events. Nonetheless, RFC1 polymorphisms have drawn a lot of interest in current medical genetics studies. The objectives of the study were to ascertain the relationship between the risk of acute lymphoblastic leukemia in Sudanese patients and the genetic variant of RFC1 (G 80A). Using Real-Time Polymerase Chain Reaction (RT-PCR) method, 150 Acute Lymphoblastic Leukemia (ALL) patients and 150 healthy control volunteers had their DNA collected and examined for the case-control study. Version 25 of the Statistical Package for Social Science (SPSS) software was used to analyze the data. There was no discernible difference in the age or gender of the patients compared to the controls. According to Flow cytometry data, T-ALL accounts for 31 (21%) and B cell type accounts for 119 (79%). According to this study, there is a statistically significant correlation between the mean blast (p=0.050) and the mean total white blood cell count (p=0.000) in T-ALL cases. The genotype frequency of RFC1 (G80A) was found to be GG 39 (26.0%), GA 41 (27.3%), and 70 (46.7%) for AA in the cases, while GG 41 (27.3%), GA 28 (18.7%), and AA 81 (54.0%) in the control group. While the homozygous genotypes AA and GG were more common in the control group, the heterozygous genotype GA was more common in the patient group. On the other hand, the genotype distribution among the research groups was not statistically significant with odds ratio: 0.745 (0.473-1.174), p=0.192. In conclusion, RFC1 (G80A) polymorphism was not found to be associated with an increased risk of ALL, according to the findings of this investigation. https://www.pagepressjournals.org/jbr/article/view/13105Acute lymphoblastic leukemiaReduced Folate Carrier1 (RFC1)RFC1 (G80A) polymorphism |
| spellingShingle | Mashaer Taha Edris Maye Mohammad Merghani Shaza Salih Gafar Ahmed Mohamed Asmeli Elrashed B. Yasin Raed Alserihi Haitham M.H. Qutob Aymen Yasin Elshazali Ali Reduced folate carrier 1 (<i>RFC1</i>) gene polymorphisms among acute lymphoblastic leukemia patients Journal of Biological Research Acute lymphoblastic leukemia Reduced Folate Carrier1 (RFC1) RFC1 (G80A) polymorphism |
| title | Reduced folate carrier 1 (<i>RFC1</i>) gene polymorphisms among acute lymphoblastic leukemia patients |
| title_full | Reduced folate carrier 1 (<i>RFC1</i>) gene polymorphisms among acute lymphoblastic leukemia patients |
| title_fullStr | Reduced folate carrier 1 (<i>RFC1</i>) gene polymorphisms among acute lymphoblastic leukemia patients |
| title_full_unstemmed | Reduced folate carrier 1 (<i>RFC1</i>) gene polymorphisms among acute lymphoblastic leukemia patients |
| title_short | Reduced folate carrier 1 (<i>RFC1</i>) gene polymorphisms among acute lymphoblastic leukemia patients |
| title_sort | reduced folate carrier 1 i rfc1 i gene polymorphisms among acute lymphoblastic leukemia patients |
| topic | Acute lymphoblastic leukemia Reduced Folate Carrier1 (RFC1) RFC1 (G80A) polymorphism |
| url | https://www.pagepressjournals.org/jbr/article/view/13105 |
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