Characterization and Potential Application of Phage vB_PmuM_CFP3 for Phage Therapy Against Avian <i>Pasteurella multocida</i>
The rise of antibiotic-resistant bacterial infections necessitates alternative therapeutic strategies, such as phage therapy. This study investigates the potential of phage vB_PmuM_CFP3 (CFP3) as a therapeutic agent against avian cholera caused by <i>Pasteurella multocida</i> (<i>P...
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2024-11-01
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| author | Hongmei Chen Nansong Jiang Guanghua Fu Qiuling Fu Chunhe Wan Yu Huang Yuan Liu Rongchang Liu Qizhang Liang Longfei Cheng |
| author_facet | Hongmei Chen Nansong Jiang Guanghua Fu Qiuling Fu Chunhe Wan Yu Huang Yuan Liu Rongchang Liu Qizhang Liang Longfei Cheng |
| author_sort | Hongmei Chen |
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| description | The rise of antibiotic-resistant bacterial infections necessitates alternative therapeutic strategies, such as phage therapy. This study investigates the potential of phage vB_PmuM_CFP3 (CFP3) as a therapeutic agent against avian cholera caused by <i>Pasteurella multocida</i> (<i>P. multocida</i>). Phage CFP3 was isolated from the feces and wastewater of a laying hen farm and underwent comprehensive biological characterization, including host range, lytic activity, and environmental stability. Transmission electron microscopy revealed CFP3′s typical myovirus morphology, with a head diameter of approximately 60 nm and a tail length of about 120 nm. CFP3 demonstrated high stability across a pH range of 4–10 and temperatures of 30–40 °C, making it suitable for oral administration in poultry. The phage exhibited a latent period of about 90 min and an optimal multiplicity of infection (MOI) of 1. Despite its narrow host range, with a lysis rate of 28.2% against avian-derived type A <i>P. multocida</i>, CFP3′s specificity minimizes impact on non-target bacteria. Whole-genome sequencing revealed a 32,696 bp linear double-stranded DNA genome with 46 predicted open reading frames (ORFs) and no tRNA or antibiotic resistance genes, enhancing its safety profile. Phylogenetic analysis indicated a close evolutionary relationship with <i>Haemophilus</i> phages HP1, HP2, and <i>Pasteurella</i> phage F108. While CFP3 shows promise as a precision therapeutic tool, further in vivo studies are required to evaluate its efficacy and safety. Future research should focus on expanding the phage library, optimizing phage mixtures, and exploring synergistic effects with other antimicrobial strategies. This study provides foundational data supporting the development of CFP3 as a viable alternative to antibiotics for controlling avian cholera. |
| format | Article |
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| language | English |
| publishDate | 2024-11-01 |
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| series | Animals |
| spelling | doaj-art-c1dae4ec4cbb461d9535d3ddf502eebf2025-08-20T02:26:52ZengMDPI AGAnimals2076-26152024-11-011422326810.3390/ani14223268Characterization and Potential Application of Phage vB_PmuM_CFP3 for Phage Therapy Against Avian <i>Pasteurella multocida</i>Hongmei Chen0Nansong Jiang1Guanghua Fu2Qiuling Fu3Chunhe Wan4Yu Huang5Yuan Liu6Rongchang Liu7Qizhang Liang8Longfei Cheng9Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou 225009, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaThe rise of antibiotic-resistant bacterial infections necessitates alternative therapeutic strategies, such as phage therapy. This study investigates the potential of phage vB_PmuM_CFP3 (CFP3) as a therapeutic agent against avian cholera caused by <i>Pasteurella multocida</i> (<i>P. multocida</i>). Phage CFP3 was isolated from the feces and wastewater of a laying hen farm and underwent comprehensive biological characterization, including host range, lytic activity, and environmental stability. Transmission electron microscopy revealed CFP3′s typical myovirus morphology, with a head diameter of approximately 60 nm and a tail length of about 120 nm. CFP3 demonstrated high stability across a pH range of 4–10 and temperatures of 30–40 °C, making it suitable for oral administration in poultry. The phage exhibited a latent period of about 90 min and an optimal multiplicity of infection (MOI) of 1. Despite its narrow host range, with a lysis rate of 28.2% against avian-derived type A <i>P. multocida</i>, CFP3′s specificity minimizes impact on non-target bacteria. Whole-genome sequencing revealed a 32,696 bp linear double-stranded DNA genome with 46 predicted open reading frames (ORFs) and no tRNA or antibiotic resistance genes, enhancing its safety profile. Phylogenetic analysis indicated a close evolutionary relationship with <i>Haemophilus</i> phages HP1, HP2, and <i>Pasteurella</i> phage F108. While CFP3 shows promise as a precision therapeutic tool, further in vivo studies are required to evaluate its efficacy and safety. Future research should focus on expanding the phage library, optimizing phage mixtures, and exploring synergistic effects with other antimicrobial strategies. This study provides foundational data supporting the development of CFP3 as a viable alternative to antibiotics for controlling avian cholera.https://www.mdpi.com/2076-2615/14/22/3268phage CFP3avian cholera<i>Pasteurella multocida</i>phage therapyantibiotic alternative |
| spellingShingle | Hongmei Chen Nansong Jiang Guanghua Fu Qiuling Fu Chunhe Wan Yu Huang Yuan Liu Rongchang Liu Qizhang Liang Longfei Cheng Characterization and Potential Application of Phage vB_PmuM_CFP3 for Phage Therapy Against Avian <i>Pasteurella multocida</i> Animals phage CFP3 avian cholera <i>Pasteurella multocida</i> phage therapy antibiotic alternative |
| title | Characterization and Potential Application of Phage vB_PmuM_CFP3 for Phage Therapy Against Avian <i>Pasteurella multocida</i> |
| title_full | Characterization and Potential Application of Phage vB_PmuM_CFP3 for Phage Therapy Against Avian <i>Pasteurella multocida</i> |
| title_fullStr | Characterization and Potential Application of Phage vB_PmuM_CFP3 for Phage Therapy Against Avian <i>Pasteurella multocida</i> |
| title_full_unstemmed | Characterization and Potential Application of Phage vB_PmuM_CFP3 for Phage Therapy Against Avian <i>Pasteurella multocida</i> |
| title_short | Characterization and Potential Application of Phage vB_PmuM_CFP3 for Phage Therapy Against Avian <i>Pasteurella multocida</i> |
| title_sort | characterization and potential application of phage vb pmum cfp3 for phage therapy against avian i pasteurella multocida i |
| topic | phage CFP3 avian cholera <i>Pasteurella multocida</i> phage therapy antibiotic alternative |
| url | https://www.mdpi.com/2076-2615/14/22/3268 |
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