Simultaneous detection of mRNA and protein in single cells using immunofluorescence-combined single-molecule RNA FISH

Although the concept of combining immunofluorescence (IF) with single-molecule RNA fluorescence in situ hybridization (smRNA FISH) seems obvious, the specific materials used during IF and smRNA FISH make it difficult to perform these procedures simultaneously on the same specimen. Even though there...

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Bibliographic Details
Main Authors: Jakub Kochan, Mateusz Wawro, Aneta Kasza
Format: Article
Language:English
Published: Taylor & Francis Group 2015-10-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/000114340
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