DNA origami signal amplification in lateral flow immunoassays
Abstract Lateral flow immunoassays (LFIAs) enable a rapid detection of analytes in a simple, paper-based test format. Despite their multiple advantages, such as low cost and ease of use, their low sensitivity compared to laboratory-based testing limits their use in e.g. many critical point-of-care a...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-04-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-57385-6 |
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| author | Heini Ijäs Julian Trommler Linh Nguyen Stefan van Rest Philipp C. Nickels Tim Liedl Maximilian J. Urban |
| author_facet | Heini Ijäs Julian Trommler Linh Nguyen Stefan van Rest Philipp C. Nickels Tim Liedl Maximilian J. Urban |
| author_sort | Heini Ijäs |
| collection | DOAJ |
| description | Abstract Lateral flow immunoassays (LFIAs) enable a rapid detection of analytes in a simple, paper-based test format. Despite their multiple advantages, such as low cost and ease of use, their low sensitivity compared to laboratory-based testing limits their use in e.g. many critical point-of-care applications. Here, we present a DNA origami-based signal amplification technology for LFIAs. DNA origami is used as a molecularly precise adapter to connect detection antibodies to tailored numbers of signal-generating labels. As a proof of concept, we apply the DNA origami signal amplification in a sandwich-based LFIA for the detection of cardiac troponin I (cTnI) in human serum. We show a 55-fold improvement of the assay sensitivity with 40-nm gold nanoparticle labels and an adjustable signal amplification of up to 125-fold with fluorescent dyes. The technology is compatible with a wide range of existing analytes, labels, and sample matrices, and presents a modular approach for improving the sensitivity and reliability of lateral flow testing. |
| format | Article |
| id | doaj-art-c1cea6843c914a75adeade01d93dc394 |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-c1cea6843c914a75adeade01d93dc3942025-08-20T02:25:41ZengNature PortfolioNature Communications2041-17232025-04-0116111010.1038/s41467-025-57385-6DNA origami signal amplification in lateral flow immunoassaysHeini Ijäs0Julian Trommler1Linh Nguyen2Stefan van Rest3Philipp C. Nickels4Tim Liedl5Maximilian J. Urban6Faculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität MünchenFaculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität MünchenFaculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität MünchenFaculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität MünchenFaculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität MünchenFaculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität MünchenFaculty of Physics and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität MünchenAbstract Lateral flow immunoassays (LFIAs) enable a rapid detection of analytes in a simple, paper-based test format. Despite their multiple advantages, such as low cost and ease of use, their low sensitivity compared to laboratory-based testing limits their use in e.g. many critical point-of-care applications. Here, we present a DNA origami-based signal amplification technology for LFIAs. DNA origami is used as a molecularly precise adapter to connect detection antibodies to tailored numbers of signal-generating labels. As a proof of concept, we apply the DNA origami signal amplification in a sandwich-based LFIA for the detection of cardiac troponin I (cTnI) in human serum. We show a 55-fold improvement of the assay sensitivity with 40-nm gold nanoparticle labels and an adjustable signal amplification of up to 125-fold with fluorescent dyes. The technology is compatible with a wide range of existing analytes, labels, and sample matrices, and presents a modular approach for improving the sensitivity and reliability of lateral flow testing.https://doi.org/10.1038/s41467-025-57385-6 |
| spellingShingle | Heini Ijäs Julian Trommler Linh Nguyen Stefan van Rest Philipp C. Nickels Tim Liedl Maximilian J. Urban DNA origami signal amplification in lateral flow immunoassays Nature Communications |
| title | DNA origami signal amplification in lateral flow immunoassays |
| title_full | DNA origami signal amplification in lateral flow immunoassays |
| title_fullStr | DNA origami signal amplification in lateral flow immunoassays |
| title_full_unstemmed | DNA origami signal amplification in lateral flow immunoassays |
| title_short | DNA origami signal amplification in lateral flow immunoassays |
| title_sort | dna origami signal amplification in lateral flow immunoassays |
| url | https://doi.org/10.1038/s41467-025-57385-6 |
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