Application of human iPSC-derived white, beige, and brown adipocytes for metabolic disease modeling and transplantation therapy
Adipocyte dysfunction plays a critical role in the pathogenesis of metabolic diseases, including type 2 diabetes (T2D). Human induced pluripotent stem cells (hiPSCs) offer a powerful platform for generating white, beige, and brown adipocytes, supporting both disease modeling and therapeutic research...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-06-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/09636897251346599 |
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| _version_ | 1850217765013553152 |
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| author | Yamato Keidai Junji Fujikura Daisuke Yabe |
| author_facet | Yamato Keidai Junji Fujikura Daisuke Yabe |
| author_sort | Yamato Keidai |
| collection | DOAJ |
| description | Adipocyte dysfunction plays a critical role in the pathogenesis of metabolic diseases, including type 2 diabetes (T2D). Human induced pluripotent stem cells (hiPSCs) offer a powerful platform for generating white, beige, and brown adipocytes, supporting both disease modeling and therapeutic research. This review provides a comprehensive summary of current differentiation methods to produce three functionally mature adipocyte types from pluripotent stem cells (PSCs), including forced gene expression techniques, developmental biology-inspired approaches, and advanced three-dimensional (3D) culture systems that enhance cellular maturity and functional relevance. PSC-derived white adipocytes contribute to modeling adipocyte dysfunction not only in conditions such as insulin resistance, lipodystrophy, and premature aging but also in more complex metabolic diseases, including T2D, facilitating the investigation of disease mechanisms and the identification of novel therapeutic targets. In addition, iPSC-based models provide a robust platform for exploring genetic regulation by genome-wide association studies (GWAS)–identified variants through population genetics. This review also evaluates the therapeutic potential of iPSC-derived white, beige, and brown adipocytes in cell transplantation therapy for metabolic diseases, with a focus on engraftment potential and metabolic improvement. Enhancing the maturity and subtype specificity of PSC-derived adipocytes is expected to accelerate the development of personalized medicine and innovative therapeutic strategies for metabolic diseases. |
| format | Article |
| id | doaj-art-c1ca0d32e604441b8e18cfc671cae422 |
| institution | OA Journals |
| issn | 1555-3892 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj-art-c1ca0d32e604441b8e18cfc671cae4222025-08-20T02:07:59ZengSAGE PublishingCell Transplantation1555-38922025-06-013410.1177/09636897251346599Application of human iPSC-derived white, beige, and brown adipocytes for metabolic disease modeling and transplantation therapyYamato Keidai0Junji Fujikura1Daisuke Yabe2Department of Diabetes and Endocrinology, Medical Research Institute Kitano Hospital, PIIF Tazuke-Kofukai, Osaka, JapanDepartment of Diabetes, Endocrinology and Nutrition, Graduate School of Medicine, Kyoto University, Kyoto, JapanCenter for One Medicine Innovative Translational Research, Gifu University Institute for Advanced Studies, Gifu, JapanAdipocyte dysfunction plays a critical role in the pathogenesis of metabolic diseases, including type 2 diabetes (T2D). Human induced pluripotent stem cells (hiPSCs) offer a powerful platform for generating white, beige, and brown adipocytes, supporting both disease modeling and therapeutic research. This review provides a comprehensive summary of current differentiation methods to produce three functionally mature adipocyte types from pluripotent stem cells (PSCs), including forced gene expression techniques, developmental biology-inspired approaches, and advanced three-dimensional (3D) culture systems that enhance cellular maturity and functional relevance. PSC-derived white adipocytes contribute to modeling adipocyte dysfunction not only in conditions such as insulin resistance, lipodystrophy, and premature aging but also in more complex metabolic diseases, including T2D, facilitating the investigation of disease mechanisms and the identification of novel therapeutic targets. In addition, iPSC-based models provide a robust platform for exploring genetic regulation by genome-wide association studies (GWAS)–identified variants through population genetics. This review also evaluates the therapeutic potential of iPSC-derived white, beige, and brown adipocytes in cell transplantation therapy for metabolic diseases, with a focus on engraftment potential and metabolic improvement. Enhancing the maturity and subtype specificity of PSC-derived adipocytes is expected to accelerate the development of personalized medicine and innovative therapeutic strategies for metabolic diseases.https://doi.org/10.1177/09636897251346599 |
| spellingShingle | Yamato Keidai Junji Fujikura Daisuke Yabe Application of human iPSC-derived white, beige, and brown adipocytes for metabolic disease modeling and transplantation therapy Cell Transplantation |
| title | Application of human iPSC-derived white, beige, and brown adipocytes for metabolic disease modeling and transplantation therapy |
| title_full | Application of human iPSC-derived white, beige, and brown adipocytes for metabolic disease modeling and transplantation therapy |
| title_fullStr | Application of human iPSC-derived white, beige, and brown adipocytes for metabolic disease modeling and transplantation therapy |
| title_full_unstemmed | Application of human iPSC-derived white, beige, and brown adipocytes for metabolic disease modeling and transplantation therapy |
| title_short | Application of human iPSC-derived white, beige, and brown adipocytes for metabolic disease modeling and transplantation therapy |
| title_sort | application of human ipsc derived white beige and brown adipocytes for metabolic disease modeling and transplantation therapy |
| url | https://doi.org/10.1177/09636897251346599 |
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