Effect of ultrasound-assisted high-pressure treatment on taste-active compounds in bovine bone soup based on peptidomics analysis

In recent years, ultrasonic-assisted technology has garnered significant attention for its potential to enhance flavor characteristics. However, the specific effects of ultrasound-assisted high-pressure processing treatment (UHBBS) on flavor development, particularly concerning taste-active compound...

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Main Authors: Haixia Ye, Shikun Suo, Yanli Wang, Daodong Pan, Qiang Xia, Xinchang Gao, Yali Dang
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Ultrasonics Sonochemistry
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Online Access:http://www.sciencedirect.com/science/article/pii/S1350417725002767
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author Haixia Ye
Shikun Suo
Yanli Wang
Daodong Pan
Qiang Xia
Xinchang Gao
Yali Dang
author_facet Haixia Ye
Shikun Suo
Yanli Wang
Daodong Pan
Qiang Xia
Xinchang Gao
Yali Dang
author_sort Haixia Ye
collection DOAJ
description In recent years, ultrasonic-assisted technology has garnered significant attention for its potential to enhance flavor characteristics. However, the specific effects of ultrasound-assisted high-pressure processing treatment (UHBBS) on flavor development, particularly concerning taste-active compounds, remain insufficiently elucidated. This study innovatively applies UHBBS treatment to the boiling of bovine bone soup and utilizes peptidomics methods to investigate the effects of UHBBS on the composition of taste-active compounds and peptide profiles within the soup. The results indicated a significant enhancement in the umami intensity due to UHBBS, primarily attributed to increased levels of taste-active compounds. In comparison to high-pressure treatment (HBBS), nucleotides content rose by 34.18 %, while free amino acids (FAAs) exhibited an increase of 150.62 %. UHBBS treatment also induced alterations in the peptide spectrum, resulting in the generation of 456 unique peptides. Among the 246 shared peptides, the abundance of 31.70 % exhibited significant changes. Furthermore, UHBBS treatment facilitated the partial unfolding of proteins, which exposed additional cleavage sites, enriching the presence of acetylated peptides. Notably, peptide acetylation predominantly occurs on alanine residues. These findings suggested that UHBBS treatment not only enhanced the content of taste-active compounds but also increased their diversity, offering a promising strategy for optimizing bone broth processing.
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issn 1350-4177
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publishDate 2025-09-01
publisher Elsevier
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series Ultrasonics Sonochemistry
spelling doaj-art-c1bfbe9ccb634b5385025b50cd4515882025-08-20T05:04:51ZengElsevierUltrasonics Sonochemistry1350-41772025-09-0112010749710.1016/j.ultsonch.2025.107497Effect of ultrasound-assisted high-pressure treatment on taste-active compounds in bovine bone soup based on peptidomics analysisHaixia Ye0Shikun Suo1Yanli Wang2Daodong Pan3Qiang Xia4Xinchang Gao5Yali Dang6Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, College of Food Science and Engineering, Ningbo University, Ningbo 315211, ChinaZhejiang Key Laboratory of Intelligent Food Logistic and Processing, College of Food Science and Engineering, Ningbo University, Ningbo 315211, ChinaZhejiang Key Laboratory of Intelligent Food Logistic and Processing, College of Food Science and Engineering, Ningbo University, Ningbo 315211, ChinaZhejiang Key Laboratory of Intelligent Food Logistic and Processing, College of Food Science and Engineering, Ningbo University, Ningbo 315211, ChinaZhejiang Key Laboratory of Intelligent Food Logistic and Processing, College of Food Science and Engineering, Ningbo University, Ningbo 315211, ChinaInstitute of Drug Discovery Technology, Ningbo University, Ningbo, Zhejiang 315211, China; Corresponding authors.Zhejiang Key Laboratory of Intelligent Food Logistic and Processing, College of Food Science and Engineering, Ningbo University, Ningbo 315211, China; Corresponding authors.In recent years, ultrasonic-assisted technology has garnered significant attention for its potential to enhance flavor characteristics. However, the specific effects of ultrasound-assisted high-pressure processing treatment (UHBBS) on flavor development, particularly concerning taste-active compounds, remain insufficiently elucidated. This study innovatively applies UHBBS treatment to the boiling of bovine bone soup and utilizes peptidomics methods to investigate the effects of UHBBS on the composition of taste-active compounds and peptide profiles within the soup. The results indicated a significant enhancement in the umami intensity due to UHBBS, primarily attributed to increased levels of taste-active compounds. In comparison to high-pressure treatment (HBBS), nucleotides content rose by 34.18 %, while free amino acids (FAAs) exhibited an increase of 150.62 %. UHBBS treatment also induced alterations in the peptide spectrum, resulting in the generation of 456 unique peptides. Among the 246 shared peptides, the abundance of 31.70 % exhibited significant changes. Furthermore, UHBBS treatment facilitated the partial unfolding of proteins, which exposed additional cleavage sites, enriching the presence of acetylated peptides. Notably, peptide acetylation predominantly occurs on alanine residues. These findings suggested that UHBBS treatment not only enhanced the content of taste-active compounds but also increased their diversity, offering a promising strategy for optimizing bone broth processing.http://www.sciencedirect.com/science/article/pii/S1350417725002767Ultrasonic-assisted high-pressure treatmentBovine bone soupTaste-active compoundsPeptide profilingPeptidomics analysis
spellingShingle Haixia Ye
Shikun Suo
Yanli Wang
Daodong Pan
Qiang Xia
Xinchang Gao
Yali Dang
Effect of ultrasound-assisted high-pressure treatment on taste-active compounds in bovine bone soup based on peptidomics analysis
Ultrasonics Sonochemistry
Ultrasonic-assisted high-pressure treatment
Bovine bone soup
Taste-active compounds
Peptide profiling
Peptidomics analysis
title Effect of ultrasound-assisted high-pressure treatment on taste-active compounds in bovine bone soup based on peptidomics analysis
title_full Effect of ultrasound-assisted high-pressure treatment on taste-active compounds in bovine bone soup based on peptidomics analysis
title_fullStr Effect of ultrasound-assisted high-pressure treatment on taste-active compounds in bovine bone soup based on peptidomics analysis
title_full_unstemmed Effect of ultrasound-assisted high-pressure treatment on taste-active compounds in bovine bone soup based on peptidomics analysis
title_short Effect of ultrasound-assisted high-pressure treatment on taste-active compounds in bovine bone soup based on peptidomics analysis
title_sort effect of ultrasound assisted high pressure treatment on taste active compounds in bovine bone soup based on peptidomics analysis
topic Ultrasonic-assisted high-pressure treatment
Bovine bone soup
Taste-active compounds
Peptide profiling
Peptidomics analysis
url http://www.sciencedirect.com/science/article/pii/S1350417725002767
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