Melamine Exacerbates Neurotoxicity in D-Galactose-Induced Neuronal SH-SY5Y Cells
Numerous studies have depicted the role of diet and environmental toxins in aging. Melamine (Mel) is a globally known notorious food adulterant, and its toxicity has been shown in several organs including the brain. However, till now, there are no reports regarding Mel neurotoxicity in aging neurons...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Journal of Aging Research |
| Online Access: | http://dx.doi.org/10.1155/2023/6635370 |
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| author | Juhi Goyal Preet Jain Vivek Jain Dibyajyoti Banerjee Rajasri Bhattacharyya Sharmistha Dey Rambabu Sharma Nitish Rai |
| author_facet | Juhi Goyal Preet Jain Vivek Jain Dibyajyoti Banerjee Rajasri Bhattacharyya Sharmistha Dey Rambabu Sharma Nitish Rai |
| author_sort | Juhi Goyal |
| collection | DOAJ |
| description | Numerous studies have depicted the role of diet and environmental toxins in aging. Melamine (Mel) is a globally known notorious food adulterant, and its toxicity has been shown in several organs including the brain. However, till now, there are no reports regarding Mel neurotoxicity in aging neurons. So, this study examined the in vitro neurotoxicity caused by Mel in the D-galactose (DG)-induced aging model of neuronal SH-SY5Y cells. In the present study, the neuronal SH-SY5Y cells were treated with DG and Mel separately and in combination to assess the neurotoxicity potential using MTT assay and neurite length measurement. Further, the superoxide dismutase (SOD), catalase (CAT), and total antioxidant activities were evaluated followed by the determination of the intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and caspase3 (Casp3) activity. The cotreatment of Mel and DG in neuronal SH-SY5Y cells showed maximum cell death than the cells treated with DG or Mel individually and untreated control cells. The neurite length shrinkage and ROS production were maximum in the DG and Mel cotreated cells showing exacerbated toxicity of Mel. The activity of SOD, CAT, and total antioxidants was also found to be lowered in the cotreatment group (Mel + DG) than in Mel- or DG-treated and untreated cells. Further, the combined toxicity of Mel and DG also elevated the Casp3 activity more than any other group. This is the first study showing the increased neurotoxic potential of Mel in an aging model of neuronal SH-SY5Y cells which implicates that Mel consumption by the elderly may lead to increased incidences of neurodegeneration like Alzheimer’s disease and Parkinson’s disease. |
| format | Article |
| id | doaj-art-c1ab9c6febcf4bc091a1fc49dcdf42df |
| institution | DOAJ |
| issn | 2090-2212 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Aging Research |
| spelling | doaj-art-c1ab9c6febcf4bc091a1fc49dcdf42df2025-08-20T03:23:30ZengWileyJournal of Aging Research2090-22122023-01-01202310.1155/2023/6635370Melamine Exacerbates Neurotoxicity in D-Galactose-Induced Neuronal SH-SY5Y CellsJuhi Goyal0Preet Jain1Vivek Jain2Dibyajyoti Banerjee3Rajasri Bhattacharyya4Sharmistha Dey5Rambabu Sharma6Nitish Rai7Department of BiotechnologyDepartment of BiotechnologyDepartment of Pharmaceutical SciencesDepartment of Experimental Medicine and BiotechnologyDepartment of Experimental Medicine and BiotechnologyDepartment of BiophysicsDepartment of MicrobiologyDepartment of BiotechnologyNumerous studies have depicted the role of diet and environmental toxins in aging. Melamine (Mel) is a globally known notorious food adulterant, and its toxicity has been shown in several organs including the brain. However, till now, there are no reports regarding Mel neurotoxicity in aging neurons. So, this study examined the in vitro neurotoxicity caused by Mel in the D-galactose (DG)-induced aging model of neuronal SH-SY5Y cells. In the present study, the neuronal SH-SY5Y cells were treated with DG and Mel separately and in combination to assess the neurotoxicity potential using MTT assay and neurite length measurement. Further, the superoxide dismutase (SOD), catalase (CAT), and total antioxidant activities were evaluated followed by the determination of the intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and caspase3 (Casp3) activity. The cotreatment of Mel and DG in neuronal SH-SY5Y cells showed maximum cell death than the cells treated with DG or Mel individually and untreated control cells. The neurite length shrinkage and ROS production were maximum in the DG and Mel cotreated cells showing exacerbated toxicity of Mel. The activity of SOD, CAT, and total antioxidants was also found to be lowered in the cotreatment group (Mel + DG) than in Mel- or DG-treated and untreated cells. Further, the combined toxicity of Mel and DG also elevated the Casp3 activity more than any other group. This is the first study showing the increased neurotoxic potential of Mel in an aging model of neuronal SH-SY5Y cells which implicates that Mel consumption by the elderly may lead to increased incidences of neurodegeneration like Alzheimer’s disease and Parkinson’s disease.http://dx.doi.org/10.1155/2023/6635370 |
| spellingShingle | Juhi Goyal Preet Jain Vivek Jain Dibyajyoti Banerjee Rajasri Bhattacharyya Sharmistha Dey Rambabu Sharma Nitish Rai Melamine Exacerbates Neurotoxicity in D-Galactose-Induced Neuronal SH-SY5Y Cells Journal of Aging Research |
| title | Melamine Exacerbates Neurotoxicity in D-Galactose-Induced Neuronal SH-SY5Y Cells |
| title_full | Melamine Exacerbates Neurotoxicity in D-Galactose-Induced Neuronal SH-SY5Y Cells |
| title_fullStr | Melamine Exacerbates Neurotoxicity in D-Galactose-Induced Neuronal SH-SY5Y Cells |
| title_full_unstemmed | Melamine Exacerbates Neurotoxicity in D-Galactose-Induced Neuronal SH-SY5Y Cells |
| title_short | Melamine Exacerbates Neurotoxicity in D-Galactose-Induced Neuronal SH-SY5Y Cells |
| title_sort | melamine exacerbates neurotoxicity in d galactose induced neuronal sh sy5y cells |
| url | http://dx.doi.org/10.1155/2023/6635370 |
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