Contact and Non-Contact Measurements of Chlorophyll-a in Water Based on Laser Spectroscopy
Chlorophyll-a fluorescence detection is an important technique for monitoring water quality. In this paper, we proposed an approach that employs the ratio of fluorescence to Raman obtained from contact and non-contact laser-induced fluorescence detection methods as the key for the detection of Chlor...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Journal of Marine Science and Engineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-1312/13/2/293 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849722403186278400 |
|---|---|
| author | Xiuyue Dong Xiaohua Che Zhaoshuo Tian |
| author_facet | Xiuyue Dong Xiaohua Che Zhaoshuo Tian |
| author_sort | Xiuyue Dong |
| collection | DOAJ |
| description | Chlorophyll-a fluorescence detection is an important technique for monitoring water quality. In this paper, we proposed an approach that employs the ratio of fluorescence to Raman obtained from contact and non-contact laser-induced fluorescence detection methods as the key for the detection of Chlorophyll-a in water. At first, extracted Chlorophyll-a solutions were prepared, and each sample was tested using two detection methods. The true values of the samples were measured in the laboratory using spectrophotometry. Then, the detection system was calibrated through the linear fitting of Chlorophyll-a and the fluorescence–Raman ratio. The linear correlation coefficients of contact and non-contact detection were 0.9453 and 0.9401, respectively. Finally, we tested the actual water samples in two ways, and compared the test results with the value measured using the national standard method. The root mean square error (RMSE) of contact and non-contact detection was 0.16 and 0.23, respectively. The results show that the two detection methods have high accuracy and sensitivity, and preliminary preparation of samples is not required. Compared with contact detection, the non-contact detection results had higher accuracy and stronger anti-interference, but the maintenance cost was higher because the probe is vulnerable to wear. The advantage of non-contact detection is that it avoids sample contamination and is easy to apply over large areas of water. In the future, it can be used for the real-time monitoring of algal biomass in water by selecting the appropriate detection method according to the requirements of the application. |
| format | Article |
| id | doaj-art-c1a832f53af74f2db83118b64c5a1dfc |
| institution | DOAJ |
| issn | 2077-1312 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj-art-c1a832f53af74f2db83118b64c5a1dfc2025-08-20T03:11:21ZengMDPI AGJournal of Marine Science and Engineering2077-13122025-02-0113229310.3390/jmse13020293Contact and Non-Contact Measurements of Chlorophyll-a in Water Based on Laser SpectroscopyXiuyue Dong0Xiaohua Che1Zhaoshuo Tian2School of Marine Science and Technology, Harbin Institute of Technology, Weihai 264209, ChinaSchool of Information Science and Engineering, Harbin Institute of Technology, Weihai 264209, ChinaSchool of Information Science and Engineering, Harbin Institute of Technology, Weihai 264209, ChinaChlorophyll-a fluorescence detection is an important technique for monitoring water quality. In this paper, we proposed an approach that employs the ratio of fluorescence to Raman obtained from contact and non-contact laser-induced fluorescence detection methods as the key for the detection of Chlorophyll-a in water. At first, extracted Chlorophyll-a solutions were prepared, and each sample was tested using two detection methods. The true values of the samples were measured in the laboratory using spectrophotometry. Then, the detection system was calibrated through the linear fitting of Chlorophyll-a and the fluorescence–Raman ratio. The linear correlation coefficients of contact and non-contact detection were 0.9453 and 0.9401, respectively. Finally, we tested the actual water samples in two ways, and compared the test results with the value measured using the national standard method. The root mean square error (RMSE) of contact and non-contact detection was 0.16 and 0.23, respectively. The results show that the two detection methods have high accuracy and sensitivity, and preliminary preparation of samples is not required. Compared with contact detection, the non-contact detection results had higher accuracy and stronger anti-interference, but the maintenance cost was higher because the probe is vulnerable to wear. The advantage of non-contact detection is that it avoids sample contamination and is easy to apply over large areas of water. In the future, it can be used for the real-time monitoring of algal biomass in water by selecting the appropriate detection method according to the requirements of the application.https://www.mdpi.com/2077-1312/13/2/293Chlorophyll-alaser induced fluorescencefluorescence spectroscopycontact and non-contact |
| spellingShingle | Xiuyue Dong Xiaohua Che Zhaoshuo Tian Contact and Non-Contact Measurements of Chlorophyll-a in Water Based on Laser Spectroscopy Journal of Marine Science and Engineering Chlorophyll-a laser induced fluorescence fluorescence spectroscopy contact and non-contact |
| title | Contact and Non-Contact Measurements of Chlorophyll-a in Water Based on Laser Spectroscopy |
| title_full | Contact and Non-Contact Measurements of Chlorophyll-a in Water Based on Laser Spectroscopy |
| title_fullStr | Contact and Non-Contact Measurements of Chlorophyll-a in Water Based on Laser Spectroscopy |
| title_full_unstemmed | Contact and Non-Contact Measurements of Chlorophyll-a in Water Based on Laser Spectroscopy |
| title_short | Contact and Non-Contact Measurements of Chlorophyll-a in Water Based on Laser Spectroscopy |
| title_sort | contact and non contact measurements of chlorophyll a in water based on laser spectroscopy |
| topic | Chlorophyll-a laser induced fluorescence fluorescence spectroscopy contact and non-contact |
| url | https://www.mdpi.com/2077-1312/13/2/293 |
| work_keys_str_mv | AT xiuyuedong contactandnoncontactmeasurementsofchlorophyllainwaterbasedonlaserspectroscopy AT xiaohuache contactandnoncontactmeasurementsofchlorophyllainwaterbasedonlaserspectroscopy AT zhaoshuotian contactandnoncontactmeasurementsofchlorophyllainwaterbasedonlaserspectroscopy |