Inflammation and oxidative stress processes in induced precocious puberty in rats
This study aimed to assess the influence of different types of blue light sources on male and female rats' puberty onset, the morphologic-induced alterations in reproductive organs tissues, the impact on inflammation and oxidative stress markers, anxiety levels, and mathematical modeling for ti...
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Elsevier
2024-12-01
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| Series: | Heliyon |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024169939 |
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| author | Raluca Maria Pop Luciana Mădălina Gherman Elena-Mihaela Jianu Ștefan Horia Roșian Mădălin Mihai Onofrei Lavinia Patricia Mocan Veronica Sanda Chedea Ioana Corina Bocsan Dragoș Apostu Andreea Roxana Todea Eva Henrietta Dulf Jeanine Cruceru Carmen Mihaela Mihu Alina Elena Pârvu Anca Dana Buzoianu |
| author_facet | Raluca Maria Pop Luciana Mădălina Gherman Elena-Mihaela Jianu Ștefan Horia Roșian Mădălin Mihai Onofrei Lavinia Patricia Mocan Veronica Sanda Chedea Ioana Corina Bocsan Dragoș Apostu Andreea Roxana Todea Eva Henrietta Dulf Jeanine Cruceru Carmen Mihaela Mihu Alina Elena Pârvu Anca Dana Buzoianu |
| author_sort | Raluca Maria Pop |
| collection | DOAJ |
| description | This study aimed to assess the influence of different types of blue light sources on male and female rats' puberty onset, the morphologic-induced alterations in reproductive organs tissues, the impact on inflammation and oxidative stress markers, anxiety levels, and mathematical modeling for tissue data interpretation. Four groups of sixteen rats each (8 females and 8 males/group) were investigated: three groups were exposed to blue light from mobile phones (MP), computer screens (PC), or LED lamps (LED) versus the control group (CTRL). The rats in the CTRL group had no exposure while the other groups were exposed for 30 days to the blue light of MP, PC, and LED for 16 h per day. Serum levels of cortisol, TNF-α, IL-6, and MMP-2 and MMP-9 ovaries and testis tissue levels were analyzed using the ELISA technique. Total oxidative stress (TOS), nitric oxide (NO), and malondialdehyde (MDA) in serum were determined spectrophotometrically. Histomorphological examination was performed on both male and female genital organs. Rats of both sexes presented significant early onset of puberty secondary to blue light exposure. LED-emitted light significantly increased TNF-α and MMP-9 levels in both sexes. The MP and PC emitted light significantly affected the levels of MMP-2 in both females and males. Levels of TOS and NO were increased by LED, respectively by MP and LED exposure in female rats. The histopathological examination revealed no statistically significant differences in the ovaries and testes of rats across the different groups. Blue light exposure induces precocious puberty, by accelerating sexual maturation, and triggers the overproduction of MMPs that could promote organic alteration through tissue remodeling. Oxidative stress parameters were upregulated only in female rats, while cortisol levels were higher in male rats. |
| format | Article |
| id | doaj-art-c1a6f6316d984f2fb2380ca69477442d |
| institution | OA Journals |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-c1a6f6316d984f2fb2380ca69477442d2025-08-20T01:58:31ZengElsevierHeliyon2405-84402024-12-011024e4096210.1016/j.heliyon.2024.e40962Inflammation and oxidative stress processes in induced precocious puberty in ratsRaluca Maria Pop0Luciana Mădălina Gherman1Elena-Mihaela Jianu2Ștefan Horia Roșian3Mădălin Mihai Onofrei4Lavinia Patricia Mocan5Veronica Sanda Chedea6Ioana Corina Bocsan7Dragoș Apostu8Andreea Roxana Todea9Eva Henrietta Dulf10Jeanine Cruceru11Carmen Mihaela Mihu12Alina Elena Pârvu13Anca Dana Buzoianu14Pharmacology, Toxicology and Clinical Pharmacology, Department of Morphofunctional Sciences, ''Iuliu Haţieganu'' University of Medicine and Pharmacy, Victor Babeș, No 8, 400012, Cluj-Napoca, Romania; Academy of Romanian Scientists, Ilfov 3, 050044, Bucharest, RomaniaAcademy of Romanian Scientists, Ilfov 3, 050044, Bucharest, Romania; Experimental Centre of ''Iuliu Haţieganu'' University of Medicine and Pharmacy, Louis Pasteur, No 6, 400349, Cluj-Napoca, Romania; Corresponding author. Academy of Romanian Scientists, Ilfov 3, 050044, Bucharest, Romania.Histology, Department of Morphofunctional Sciences, ''Iuliu Haţieganu'' University of Medicine and Pharmacy, Victor Babeș, No 8, 400012, Cluj-Napoca, Romania; Corresponding author.“Niculae Stăncioiu” Heart Institute Cluj-Napoca, 19-21 Calea Moților Street, 400001, Cluj-Napoca, Romania; Department of Cardiology—Heart Institute, “Iuliu Haţieganu” University of Medicine and Pharmacy Cluj-Napoca, Calea Moților Street No. 19-21, 400001, Cluj-Napoca, RomaniaHistology, Department of Morphofunctional Sciences, ''Iuliu Haţieganu'' University of Medicine and Pharmacy, Victor Babeș, No 8, 400012, Cluj-Napoca, RomaniaHistology, Department of Morphofunctional Sciences, ''Iuliu Haţieganu'' University of Medicine and Pharmacy, Victor Babeș, No 8, 400012, Cluj-Napoca, RomaniaResearch Station for Viticulture and Enology Blaj (SCDVV Blaj), 515400, Blaj, RomaniaPharmacology, Toxicology and Clinical Pharmacology, Department of Morphofunctional Sciences, ''Iuliu Haţieganu'' University of Medicine and Pharmacy, Victor Babeș, No 8, 400012, Cluj-Napoca, RomaniaOrthopaedics and Traumatology, Department of Surgical Specialities, Iuliu Hatieganu University of Medicine and Pharmacy, Victor Babeș, No 8, 400012, Cluj-Napoca, RomaniaDepartment of Automation, Faculty of Automation and Computer Science, Technical University of Cluj-Napoca, Memorandumului Street No. 28, 400014, Cluj-Napoca, RomaniaDepartment of Automation, Faculty of Automation and Computer Science, Technical University of Cluj-Napoca, Memorandumului Street No. 28, 400014, Cluj-Napoca, RomaniaPharmacology, Toxicology and Clinical Pharmacology, Department of Morphofunctional Sciences, ''Iuliu Haţieganu'' University of Medicine and Pharmacy, Victor Babeș, No 8, 400012, Cluj-Napoca, RomaniaHistology, Department of Morphofunctional Sciences, ''Iuliu Haţieganu'' University of Medicine and Pharmacy, Victor Babeș, No 8, 400012, Cluj-Napoca, RomaniaPathophysiology, Department of Morphofunctional Sciences, Faculty of Medicine, Iuliu Hațieganu University of Medicine and Pharmacy Cluj-Napoca, 400012 Cluj-Napoca, RomaniaPharmacology, Toxicology and Clinical Pharmacology, Department of Morphofunctional Sciences, ''Iuliu Haţieganu'' University of Medicine and Pharmacy, Victor Babeș, No 8, 400012, Cluj-Napoca, RomaniaThis study aimed to assess the influence of different types of blue light sources on male and female rats' puberty onset, the morphologic-induced alterations in reproductive organs tissues, the impact on inflammation and oxidative stress markers, anxiety levels, and mathematical modeling for tissue data interpretation. Four groups of sixteen rats each (8 females and 8 males/group) were investigated: three groups were exposed to blue light from mobile phones (MP), computer screens (PC), or LED lamps (LED) versus the control group (CTRL). The rats in the CTRL group had no exposure while the other groups were exposed for 30 days to the blue light of MP, PC, and LED for 16 h per day. Serum levels of cortisol, TNF-α, IL-6, and MMP-2 and MMP-9 ovaries and testis tissue levels were analyzed using the ELISA technique. Total oxidative stress (TOS), nitric oxide (NO), and malondialdehyde (MDA) in serum were determined spectrophotometrically. Histomorphological examination was performed on both male and female genital organs. Rats of both sexes presented significant early onset of puberty secondary to blue light exposure. LED-emitted light significantly increased TNF-α and MMP-9 levels in both sexes. The MP and PC emitted light significantly affected the levels of MMP-2 in both females and males. Levels of TOS and NO were increased by LED, respectively by MP and LED exposure in female rats. The histopathological examination revealed no statistically significant differences in the ovaries and testes of rats across the different groups. Blue light exposure induces precocious puberty, by accelerating sexual maturation, and triggers the overproduction of MMPs that could promote organic alteration through tissue remodeling. Oxidative stress parameters were upregulated only in female rats, while cortisol levels were higher in male rats.http://www.sciencedirect.com/science/article/pii/S2405844024169939 |
| spellingShingle | Raluca Maria Pop Luciana Mădălina Gherman Elena-Mihaela Jianu Ștefan Horia Roșian Mădălin Mihai Onofrei Lavinia Patricia Mocan Veronica Sanda Chedea Ioana Corina Bocsan Dragoș Apostu Andreea Roxana Todea Eva Henrietta Dulf Jeanine Cruceru Carmen Mihaela Mihu Alina Elena Pârvu Anca Dana Buzoianu Inflammation and oxidative stress processes in induced precocious puberty in rats Heliyon |
| title | Inflammation and oxidative stress processes in induced precocious puberty in rats |
| title_full | Inflammation and oxidative stress processes in induced precocious puberty in rats |
| title_fullStr | Inflammation and oxidative stress processes in induced precocious puberty in rats |
| title_full_unstemmed | Inflammation and oxidative stress processes in induced precocious puberty in rats |
| title_short | Inflammation and oxidative stress processes in induced precocious puberty in rats |
| title_sort | inflammation and oxidative stress processes in induced precocious puberty in rats |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024169939 |
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