A genome-wide investigation of SNPs and CNVs in schizophrenia.

We report a genome-wide assessment of single nucleotide polymorphisms (SNPs) and copy number variants (CNVs) in schizophrenia. We investigated SNPs using 871 patients and 863 controls, following up the top hits in four independent cohorts comprising 1,460 patients and 12,995 controls, all of Europea...

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Main Authors: Anna C Need, Dongliang Ge, Michael E Weale, Jessica Maia, Sheng Feng, Erin L Heinzen, Kevin V Shianna, Woohyun Yoon, Dalia Kasperaviciūte, Massimo Gennarelli, Warren J Strittmatter, Cristian Bonvicini, Giuseppe Rossi, Karu Jayathilake, Philip A Cola, Joseph P McEvoy, Richard S E Keefe, Elizabeth M C Fisher, Pamela L St Jean, Ina Giegling, Annette M Hartmann, Hans-Jürgen Möller, Andreas Ruppert, Gillian Fraser, Caroline Crombie, Lefkos T Middleton, David St Clair, Allen D Roses, Pierandrea Muglia, Clyde Francks, Dan Rujescu, Herbert Y Meltzer, David B Goldstein
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2009-02-01
Series:PLoS Genetics
Online Access:https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1000373&type=printable
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author Anna C Need
Dongliang Ge
Michael E Weale
Jessica Maia
Sheng Feng
Erin L Heinzen
Kevin V Shianna
Woohyun Yoon
Dalia Kasperaviciūte
Massimo Gennarelli
Warren J Strittmatter
Cristian Bonvicini
Giuseppe Rossi
Karu Jayathilake
Philip A Cola
Joseph P McEvoy
Richard S E Keefe
Elizabeth M C Fisher
Pamela L St Jean
Ina Giegling
Annette M Hartmann
Hans-Jürgen Möller
Andreas Ruppert
Gillian Fraser
Caroline Crombie
Lefkos T Middleton
David St Clair
Allen D Roses
Pierandrea Muglia
Clyde Francks
Dan Rujescu
Herbert Y Meltzer
David B Goldstein
author_facet Anna C Need
Dongliang Ge
Michael E Weale
Jessica Maia
Sheng Feng
Erin L Heinzen
Kevin V Shianna
Woohyun Yoon
Dalia Kasperaviciūte
Massimo Gennarelli
Warren J Strittmatter
Cristian Bonvicini
Giuseppe Rossi
Karu Jayathilake
Philip A Cola
Joseph P McEvoy
Richard S E Keefe
Elizabeth M C Fisher
Pamela L St Jean
Ina Giegling
Annette M Hartmann
Hans-Jürgen Möller
Andreas Ruppert
Gillian Fraser
Caroline Crombie
Lefkos T Middleton
David St Clair
Allen D Roses
Pierandrea Muglia
Clyde Francks
Dan Rujescu
Herbert Y Meltzer
David B Goldstein
author_sort Anna C Need
collection DOAJ
description We report a genome-wide assessment of single nucleotide polymorphisms (SNPs) and copy number variants (CNVs) in schizophrenia. We investigated SNPs using 871 patients and 863 controls, following up the top hits in four independent cohorts comprising 1,460 patients and 12,995 controls, all of European origin. We found no genome-wide significant associations, nor could we provide support for any previously reported candidate gene or genome-wide associations. We went on to examine CNVs using a subset of 1,013 cases and 1,084 controls of European ancestry, and a further set of 60 cases and 64 controls of African ancestry. We found that eight cases and zero controls carried deletions greater than 2 Mb, of which two, at 8p22 and 16p13.11-p12.4, are newly reported here. A further evaluation of 1,378 controls identified no deletions greater than 2 Mb, suggesting a high prior probability of disease involvement when such deletions are observed in cases. We also provide further evidence for some smaller, previously reported, schizophrenia-associated CNVs, such as those in NRXN1 and APBA2. We could not provide strong support for the hypothesis that schizophrenia patients have a significantly greater "load" of large (>100 kb), rare CNVs, nor could we find common CNVs that associate with schizophrenia. Finally, we did not provide support for the suggestion that schizophrenia-associated CNVs may preferentially disrupt genes in neurodevelopmental pathways. Collectively, these analyses provide the first integrated study of SNPs and CNVs in schizophrenia and support the emerging view that rare deleterious variants may be more important in schizophrenia predisposition than common polymorphisms. While our analyses do not suggest that implicated CNVs impinge on particular key pathways, we do support the contribution of specific genomic regions in schizophrenia, presumably due to recurrent mutation. On balance, these data suggest that very few schizophrenia patients share identical genomic causation, potentially complicating efforts to personalize treatment regimens.
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spelling doaj-art-c19e84d31375449d8e0177d2a66e2fc42025-08-20T03:22:38ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042009-02-0152e100037310.1371/journal.pgen.1000373A genome-wide investigation of SNPs and CNVs in schizophrenia.Anna C NeedDongliang GeMichael E WealeJessica MaiaSheng FengErin L HeinzenKevin V ShiannaWoohyun YoonDalia KasperaviciūteMassimo GennarelliWarren J StrittmatterCristian BonviciniGiuseppe RossiKaru JayathilakePhilip A ColaJoseph P McEvoyRichard S E KeefeElizabeth M C FisherPamela L St JeanIna GieglingAnnette M HartmannHans-Jürgen MöllerAndreas RuppertGillian FraserCaroline CrombieLefkos T MiddletonDavid St ClairAllen D RosesPierandrea MugliaClyde FrancksDan RujescuHerbert Y MeltzerDavid B GoldsteinWe report a genome-wide assessment of single nucleotide polymorphisms (SNPs) and copy number variants (CNVs) in schizophrenia. We investigated SNPs using 871 patients and 863 controls, following up the top hits in four independent cohorts comprising 1,460 patients and 12,995 controls, all of European origin. We found no genome-wide significant associations, nor could we provide support for any previously reported candidate gene or genome-wide associations. We went on to examine CNVs using a subset of 1,013 cases and 1,084 controls of European ancestry, and a further set of 60 cases and 64 controls of African ancestry. We found that eight cases and zero controls carried deletions greater than 2 Mb, of which two, at 8p22 and 16p13.11-p12.4, are newly reported here. A further evaluation of 1,378 controls identified no deletions greater than 2 Mb, suggesting a high prior probability of disease involvement when such deletions are observed in cases. We also provide further evidence for some smaller, previously reported, schizophrenia-associated CNVs, such as those in NRXN1 and APBA2. We could not provide strong support for the hypothesis that schizophrenia patients have a significantly greater "load" of large (>100 kb), rare CNVs, nor could we find common CNVs that associate with schizophrenia. Finally, we did not provide support for the suggestion that schizophrenia-associated CNVs may preferentially disrupt genes in neurodevelopmental pathways. Collectively, these analyses provide the first integrated study of SNPs and CNVs in schizophrenia and support the emerging view that rare deleterious variants may be more important in schizophrenia predisposition than common polymorphisms. While our analyses do not suggest that implicated CNVs impinge on particular key pathways, we do support the contribution of specific genomic regions in schizophrenia, presumably due to recurrent mutation. On balance, these data suggest that very few schizophrenia patients share identical genomic causation, potentially complicating efforts to personalize treatment regimens.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1000373&type=printable
spellingShingle Anna C Need
Dongliang Ge
Michael E Weale
Jessica Maia
Sheng Feng
Erin L Heinzen
Kevin V Shianna
Woohyun Yoon
Dalia Kasperaviciūte
Massimo Gennarelli
Warren J Strittmatter
Cristian Bonvicini
Giuseppe Rossi
Karu Jayathilake
Philip A Cola
Joseph P McEvoy
Richard S E Keefe
Elizabeth M C Fisher
Pamela L St Jean
Ina Giegling
Annette M Hartmann
Hans-Jürgen Möller
Andreas Ruppert
Gillian Fraser
Caroline Crombie
Lefkos T Middleton
David St Clair
Allen D Roses
Pierandrea Muglia
Clyde Francks
Dan Rujescu
Herbert Y Meltzer
David B Goldstein
A genome-wide investigation of SNPs and CNVs in schizophrenia.
PLoS Genetics
title A genome-wide investigation of SNPs and CNVs in schizophrenia.
title_full A genome-wide investigation of SNPs and CNVs in schizophrenia.
title_fullStr A genome-wide investigation of SNPs and CNVs in schizophrenia.
title_full_unstemmed A genome-wide investigation of SNPs and CNVs in schizophrenia.
title_short A genome-wide investigation of SNPs and CNVs in schizophrenia.
title_sort genome wide investigation of snps and cnvs in schizophrenia
url https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1000373&type=printable
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