Cytometric and morphological characterization of hemocytes from four species of freshwater decapod crustaceans
Characterization and classification of invertebrate hemocytes is a powerful tool for determining the physiological effect of environmental stress. Hemocytes from four decapod crustacean species Dilocarcinus pagei, Zilchiopsis collastinensis, Aegla uruguayana and Macrobrachium borellii were analyzed...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Spanish |
| Published: |
Instituto de Limnología "Raúl A. Ringuelet. (CONICET-UNLP)
2025-05-01
|
| Series: | Biología Acuática |
| Subjects: | |
| Online Access: | https://revistas.unlp.edu.ar/bacuatica/article/view/18521 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850136764379824128 |
|---|---|
| author | Paula Sofía Reyes Carlos Leandro Negro Diana Alberto María Florencia Viozzi Veronica Williner |
| author_facet | Paula Sofía Reyes Carlos Leandro Negro Diana Alberto María Florencia Viozzi Veronica Williner |
| author_sort | Paula Sofía Reyes |
| collection | DOAJ |
| description |
Characterization and classification of invertebrate hemocytes is a powerful tool for determining the physiological effect of environmental stress. Hemocytes from four decapod crustacean species Dilocarcinus pagei, Zilchiopsis collastinensis, Aegla uruguayana and Macrobrachium borellii were analyzed by flow cytometry followed by morphological characterization by optical microscopy. Based on morphological cell characteristics, three categories of hemocytes were identified: granulocytes with abundant granularity accounting for 1.87% (D. pagei), 5.23% (Z. collastinensis), 0.98% (A. uruguayana) and 2.41% (M. borellii), semigranulocytes with lower granularity accounting for 2.22% (D. pagei), 4.81% (Z. collastinensis), 2.38% (A. uruguayana) and 68.19% (M. borellii) and, finally, hyalinocytes with almost no granularity accounting for 89.87% (D. pagei), 65.75% (Z. collastinensis), 92.33% (A. uruguayana) and 22.04% (M. borellii). Analysis of the total hemocyte count (cells/ml) showed the following results: 4.3 ± 0.58 (x106) in D. pagei, 2.3 ± 0.33 (x106) in Z. collastinensis; 3.1 ± 0.56 (x106) in A. uruguayana and 2.6 ± 0.25 (x106) in M. borellii. This study demonstrates the applicability of flow cytometry to effectively differentiate and enumerate circulating hemocytes in native crustacean species, providing novel insights into the use of this method for assessing physiological status in these species. In addition, interspecific variability in these cellular parameters was observed. The results contribute to the understanding of the innate immune system in these four crustacean species and allow establishing a baseline to identify welfare or stress conditions in these animals.
|
| format | Article |
| id | doaj-art-c1957327302e4d0dbac6b1f5676aefae |
| institution | OA Journals |
| issn | 1668-4869 |
| language | Spanish |
| publishDate | 2025-05-01 |
| publisher | Instituto de Limnología "Raúl A. Ringuelet. (CONICET-UNLP) |
| record_format | Article |
| series | Biología Acuática |
| spelling | doaj-art-c1957327302e4d0dbac6b1f5676aefae2025-08-20T02:31:02ZspaInstituto de Limnología "Raúl A. Ringuelet. (CONICET-UNLP)Biología Acuática1668-48692025-05-0144 - en curso10.24215/16684869e045Cytometric and morphological characterization of hemocytes from four species of freshwater decapod crustaceansPaula Sofía Reyes0Carlos Leandro Negro1Diana Alberto2María Florencia Viozzi3Veronica Williner4Instituto Nacional de Limnología, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), ArgentinaInstituto Nacional de Limnología, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), ArgentinaInstituto Nacional de Limnología, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), ArgentinaInstituto Nacional de Limnología, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), ArgentinaInstituto Nacional de Limnología, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina Characterization and classification of invertebrate hemocytes is a powerful tool for determining the physiological effect of environmental stress. Hemocytes from four decapod crustacean species Dilocarcinus pagei, Zilchiopsis collastinensis, Aegla uruguayana and Macrobrachium borellii were analyzed by flow cytometry followed by morphological characterization by optical microscopy. Based on morphological cell characteristics, three categories of hemocytes were identified: granulocytes with abundant granularity accounting for 1.87% (D. pagei), 5.23% (Z. collastinensis), 0.98% (A. uruguayana) and 2.41% (M. borellii), semigranulocytes with lower granularity accounting for 2.22% (D. pagei), 4.81% (Z. collastinensis), 2.38% (A. uruguayana) and 68.19% (M. borellii) and, finally, hyalinocytes with almost no granularity accounting for 89.87% (D. pagei), 65.75% (Z. collastinensis), 92.33% (A. uruguayana) and 22.04% (M. borellii). Analysis of the total hemocyte count (cells/ml) showed the following results: 4.3 ± 0.58 (x106) in D. pagei, 2.3 ± 0.33 (x106) in Z. collastinensis; 3.1 ± 0.56 (x106) in A. uruguayana and 2.6 ± 0.25 (x106) in M. borellii. This study demonstrates the applicability of flow cytometry to effectively differentiate and enumerate circulating hemocytes in native crustacean species, providing novel insights into the use of this method for assessing physiological status in these species. In addition, interspecific variability in these cellular parameters was observed. The results contribute to the understanding of the innate immune system in these four crustacean species and allow establishing a baseline to identify welfare or stress conditions in these animals. https://revistas.unlp.edu.ar/bacuatica/article/view/18521Crustaceaflow cytometryhemolymphhemocyte analysis |
| spellingShingle | Paula Sofía Reyes Carlos Leandro Negro Diana Alberto María Florencia Viozzi Veronica Williner Cytometric and morphological characterization of hemocytes from four species of freshwater decapod crustaceans Biología Acuática Crustacea flow cytometry hemolymph hemocyte analysis |
| title | Cytometric and morphological characterization of hemocytes from four species of freshwater decapod crustaceans |
| title_full | Cytometric and morphological characterization of hemocytes from four species of freshwater decapod crustaceans |
| title_fullStr | Cytometric and morphological characterization of hemocytes from four species of freshwater decapod crustaceans |
| title_full_unstemmed | Cytometric and morphological characterization of hemocytes from four species of freshwater decapod crustaceans |
| title_short | Cytometric and morphological characterization of hemocytes from four species of freshwater decapod crustaceans |
| title_sort | cytometric and morphological characterization of hemocytes from four species of freshwater decapod crustaceans |
| topic | Crustacea flow cytometry hemolymph hemocyte analysis |
| url | https://revistas.unlp.edu.ar/bacuatica/article/view/18521 |
| work_keys_str_mv | AT paulasofiareyes cytometricandmorphologicalcharacterizationofhemocytesfromfourspeciesoffreshwaterdecapodcrustaceans AT carlosleandronegro cytometricandmorphologicalcharacterizationofhemocytesfromfourspeciesoffreshwaterdecapodcrustaceans AT dianaalberto cytometricandmorphologicalcharacterizationofhemocytesfromfourspeciesoffreshwaterdecapodcrustaceans AT mariaflorenciaviozzi cytometricandmorphologicalcharacterizationofhemocytesfromfourspeciesoffreshwaterdecapodcrustaceans AT veronicawilliner cytometricandmorphologicalcharacterizationofhemocytesfromfourspeciesoffreshwaterdecapodcrustaceans |