Methicillin-resistant Staphylococcus aureus in cows with mastitis, the presence of the mecA gene and the gene for virulence
The physiological properties of 47 Staphylococcus aureus strains were investigated. The test strains were grown on bacteriological media and identified by the ID32 STAF system for biochemical identification of bacteria. Sensitivity to antimicrobial agents was performed by the disc diffusion method....
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| Format: | Article |
| Language: | English |
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Croatian Dairy Union
2015-11-01
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| Series: | Mljekarstvo |
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| Online Access: | http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=218026 |
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| author | Vesna Jaki Tkalec Darko Majnarić Jadranka Jurmanović Boris Habrun Željko Cvetnić Manuela Zadravec Branka Šeol Martinec |
| author_facet | Vesna Jaki Tkalec Darko Majnarić Jadranka Jurmanović Boris Habrun Željko Cvetnić Manuela Zadravec Branka Šeol Martinec |
| author_sort | Vesna Jaki Tkalec |
| collection | DOAJ |
| description | The physiological properties of 47 Staphylococcus aureus strains were investigated. The test strains were grown on bacteriological media and identified by the ID32 STAF system for biochemical identification of bacteria. Sensitivity to antimicrobial agents was performed by the disc diffusion method. The nuc gene and the virulence factors coa, hla, hlb, hld, hlg, hlg-2, tst, eta, etb, lukF-PV and lukS-PV and mecA gene were detected by the polymerase chain reaction. Furthermore, the spa type of the studied isolates was also set. According to the obtained results, all strains had the nuc, coa, hla and hld gene. Ten strains (21.3 %) had also the tst gene, while 37 strains (78.7 %) had the hlg gene and 35 strains (74.5 %) had the hlb and hlg-2 genes. All of the investigated S. aureus isolates were penicillin resistant (100 %), with 29 strains which were also resistant to oxacillin (61.7 %). Methicillin (oxacillin) resistance was detected by the mecA gene detection, which is also the first MRSA result from the secretion samples of cows’ mammary glands in Croatia. The researched MRSA strains proved to belong to different spa types, and the most common were spa types t005, t011 and t521, and a new spa type t9498 was detected. |
| format | Article |
| id | doaj-art-c1947ce43d774a94afee3be3511eede5 |
| institution | DOAJ |
| issn | 0026-704X 1846-4025 |
| language | English |
| publishDate | 2015-11-01 |
| publisher | Croatian Dairy Union |
| record_format | Article |
| series | Mljekarstvo |
| spelling | doaj-art-c1947ce43d774a94afee3be3511eede52025-08-20T03:05:03ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252015-11-0165425926810.15567/mljekarstvo.2015.0406Methicillin-resistant Staphylococcus aureus in cows with mastitis, the presence of the mecA gene and the gene for virulenceVesna Jaki Tkalec 0Darko Majnarić1Jadranka Jurmanović2Boris Habrun3Željko Cvetnić4Manuela Zadravec5Branka Šeol Martinec6Hrvatski veterinarski institut, Veterinarski zavod Križevci, Zakmardijeva 10, 48000 Križevci, HrvatskaHrvatski veterinarski institut, Veterinarski zavod Križevci, Zakmardijeva 10, 48000 Križevci, HrvatskaHrvatski veterinarski institut, Veterinarski zavod Križevci, Zakmardijeva 10, 48000 Križevci, HrvatskaHrvatski veterinarski institut, Odjel za bakteriologiju i parazitologiju, Savska cesta 143, 10000 Zagreb, HrvatskaHrvatski veterinarski institut, Odjel za bakteriologiju i parazitologiju, Savska cesta 143, 10000 Zagreb, HrvatskaHrvatski veterinarski institut, Odjel za veterinarsko javno zdravstvo, Savska cesta 143, 10000 Zagreb, HrvatskaVeterinarski fakultet, Zavod za mikrobiologiju i zarazne bolesti s klinikom, Heinzelova 55, 10000 Zagreb, HrvatskaThe physiological properties of 47 Staphylococcus aureus strains were investigated. The test strains were grown on bacteriological media and identified by the ID32 STAF system for biochemical identification of bacteria. Sensitivity to antimicrobial agents was performed by the disc diffusion method. The nuc gene and the virulence factors coa, hla, hlb, hld, hlg, hlg-2, tst, eta, etb, lukF-PV and lukS-PV and mecA gene were detected by the polymerase chain reaction. Furthermore, the spa type of the studied isolates was also set. According to the obtained results, all strains had the nuc, coa, hla and hld gene. Ten strains (21.3 %) had also the tst gene, while 37 strains (78.7 %) had the hlg gene and 35 strains (74.5 %) had the hlb and hlg-2 genes. All of the investigated S. aureus isolates were penicillin resistant (100 %), with 29 strains which were also resistant to oxacillin (61.7 %). Methicillin (oxacillin) resistance was detected by the mecA gene detection, which is also the first MRSA result from the secretion samples of cows’ mammary glands in Croatia. The researched MRSA strains proved to belong to different spa types, and the most common were spa types t005, t011 and t521, and a new spa type t9498 was detected.http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=218026mastitic cowsStaphylococcus aureusvirulence genesmecA gene |
| spellingShingle | Vesna Jaki Tkalec Darko Majnarić Jadranka Jurmanović Boris Habrun Željko Cvetnić Manuela Zadravec Branka Šeol Martinec Methicillin-resistant Staphylococcus aureus in cows with mastitis, the presence of the mecA gene and the gene for virulence Mljekarstvo mastitic cows Staphylococcus aureus virulence genes mecA gene |
| title | Methicillin-resistant Staphylococcus aureus in cows with mastitis, the presence of the mecA gene and the gene for virulence |
| title_full | Methicillin-resistant Staphylococcus aureus in cows with mastitis, the presence of the mecA gene and the gene for virulence |
| title_fullStr | Methicillin-resistant Staphylococcus aureus in cows with mastitis, the presence of the mecA gene and the gene for virulence |
| title_full_unstemmed | Methicillin-resistant Staphylococcus aureus in cows with mastitis, the presence of the mecA gene and the gene for virulence |
| title_short | Methicillin-resistant Staphylococcus aureus in cows with mastitis, the presence of the mecA gene and the gene for virulence |
| title_sort | methicillin resistant staphylococcus aureus in cows with mastitis the presence of the meca gene and the gene for virulence |
| topic | mastitic cows Staphylococcus aureus virulence genes mecA gene |
| url | http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=218026 |
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