Validation of a triiodothyronine (T3) ELISA for mouse fecal samples
Abstract Acquiring sufficient blood for hormone analysis in mice can be a limiting step. Hormone analysis techniques using non‐invasive sample collection have been vigorously developed for endangered species, from whom blood sampling is prohibited, or from species that are otherwise difficult to han...
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| Format: | Article |
| Language: | English |
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Wiley
2024-11-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.70115 |
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| author | Lucia M. Thompson Brailey M. Coulter Cinnamon L. VanPutte |
| author_facet | Lucia M. Thompson Brailey M. Coulter Cinnamon L. VanPutte |
| author_sort | Lucia M. Thompson |
| collection | DOAJ |
| description | Abstract Acquiring sufficient blood for hormone analysis in mice can be a limiting step. Hormone analysis techniques using non‐invasive sample collection have been vigorously developed for endangered species, from whom blood sampling is prohibited, or from species that are otherwise difficult to handle in a laboratory setting. Because there are interactions between glucocorticoids and thyroid hormones (T3 and T4), reducing the animal's “distress” during sample collection is imperative. Measurement of fecal T3 provides less sensitive, baseline information regarding thyroid function while permitting a non‐invasive technique for more frequent sampling. We demonstrated that using a methanol extraction protocol produced the most reliable fecal T3 measurement in an enzyme‐linked immunosorbent assay (ELISA). We found that during a thyroid hormone‐treated state, fecal and plasma T3 measurements from mice are directly related, while during a methimazole‐treated state, fecal and plasma T3 measurements from mice are inversely related. Fecal samples are a useful way to monitor thyroid hormone function in laboratory mice. |
| format | Article |
| id | doaj-art-c1911605d9f94c72b318c9e516a26929 |
| institution | Kabale University |
| issn | 2051-817X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj-art-c1911605d9f94c72b318c9e516a269292025-01-25T06:41:00ZengWileyPhysiological Reports2051-817X2024-11-011221n/an/a10.14814/phy2.70115Validation of a triiodothyronine (T3) ELISA for mouse fecal samplesLucia M. Thompson0Brailey M. Coulter1Cinnamon L. VanPutte2Biological Sciences Southern Illinois University Edwardsville Edwardsville Illinois USABiological Sciences Southern Illinois University Edwardsville Edwardsville Illinois USABiomedical and Craniofacial Sciences Southern Illinois University School of Dental Medicine Alton Illinois USAAbstract Acquiring sufficient blood for hormone analysis in mice can be a limiting step. Hormone analysis techniques using non‐invasive sample collection have been vigorously developed for endangered species, from whom blood sampling is prohibited, or from species that are otherwise difficult to handle in a laboratory setting. Because there are interactions between glucocorticoids and thyroid hormones (T3 and T4), reducing the animal's “distress” during sample collection is imperative. Measurement of fecal T3 provides less sensitive, baseline information regarding thyroid function while permitting a non‐invasive technique for more frequent sampling. We demonstrated that using a methanol extraction protocol produced the most reliable fecal T3 measurement in an enzyme‐linked immunosorbent assay (ELISA). We found that during a thyroid hormone‐treated state, fecal and plasma T3 measurements from mice are directly related, while during a methimazole‐treated state, fecal and plasma T3 measurements from mice are inversely related. Fecal samples are a useful way to monitor thyroid hormone function in laboratory mice.https://doi.org/10.14814/phy2.70115fecal extractsmicenon‐invasive samplingperiodontal diseasethyroid hormones |
| spellingShingle | Lucia M. Thompson Brailey M. Coulter Cinnamon L. VanPutte Validation of a triiodothyronine (T3) ELISA for mouse fecal samples Physiological Reports fecal extracts mice non‐invasive sampling periodontal disease thyroid hormones |
| title | Validation of a triiodothyronine (T3) ELISA for mouse fecal samples |
| title_full | Validation of a triiodothyronine (T3) ELISA for mouse fecal samples |
| title_fullStr | Validation of a triiodothyronine (T3) ELISA for mouse fecal samples |
| title_full_unstemmed | Validation of a triiodothyronine (T3) ELISA for mouse fecal samples |
| title_short | Validation of a triiodothyronine (T3) ELISA for mouse fecal samples |
| title_sort | validation of a triiodothyronine t3 elisa for mouse fecal samples |
| topic | fecal extracts mice non‐invasive sampling periodontal disease thyroid hormones |
| url | https://doi.org/10.14814/phy2.70115 |
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