Non‐alcoholic fatty liver disease and heart failure: A comprehensive bioinformatics and Mendelian randomization analysis
Abstract Aims Heart failure (HF) and non‐alcoholic fatty liver disease (NAFLD) are significant global health issues with a complex interrelationship. This study investigates their shared biomarkers and causal relationships using bioinformatics and Mendelian randomization (MR) approaches. Methods We...
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Wiley
2024-12-01
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| Series: | ESC Heart Failure |
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| Online Access: | https://doi.org/10.1002/ehf2.15019 |
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| author | Yayun Zhang Lu Feng Xin Guan Zixiong Zhu Yubin He Xuewen Li |
| author_facet | Yayun Zhang Lu Feng Xin Guan Zixiong Zhu Yubin He Xuewen Li |
| author_sort | Yayun Zhang |
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| description | Abstract Aims Heart failure (HF) and non‐alcoholic fatty liver disease (NAFLD) are significant global health issues with a complex interrelationship. This study investigates their shared biomarkers and causal relationships using bioinformatics and Mendelian randomization (MR) approaches. Methods We analysed NAFLD and HF datasets from the Gene Expression Omnibus (GEO). The GSE126848 dataset included 57 liver biopsy samples [14 healthy individuals, 12 obese subjects, 15 NAFL patients and 16 non‐alcoholic steatohepatitis (NASH) patients]. The GSE24807 dataset comprised 12 NASH samples and 5 healthy controls. The GSE57338 dataset included 313 cardiac muscle samples [177 HF patients (95 ischaemic heart disease patients and 82 idiopathic dilated cardiomyopathy patients) and 136 healthy controls]. The GSE84796 dataset consisted of 10 end‐stage HF patients and 7 healthy hearts procured from organ donors. We identified differentially expressed genes (DEGs) and constructed a protein–protein interaction (PPI) network. Functional pathways were elucidated through enrichment analyses using Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG) and GeneMANIA annotation. Single nucleotide polymorphism (SNP) data for HF and NAFLD were sourced from genome‐wide association studies (GWAS). The HF dataset included 486 160 samples (14 262 experimental and 471 898 control), and the NAFLD dataset comprised 377 988 samples (4761 experimental and 373 227 control). MR analysis investigates the causal interrelations. Results Our analysis revealed 4032 DEGs from GSE126848 and 286 DEGs from GSE57338. The top 10 hub genes (CD163, VSIG4, CXCL10, FCER1G, FPR1, C1QB, CCR1, C1orf162, MRC1 and CD38) were significantly enriched in immune response, calcium ion concentration regulation and positive regulation of monocyte chemotaxis. CIBERSORT analysis indicated associations between these hub genes and natural killer (NK) cells and macrophages. Transcription factor (TF) target prediction for CD38, CXCL10 and CCR1 highlighted related TFs. A two‐sample MR analysis confirmed a bidirectional causal relationship between NAFLD and HF. The main method [inverse variance weighted (IVW)] demonstrated a significant positive causal relationship between NAFLD and HF [P = 0.037; odds ratio (OR) = 1.024; 95% confidence interval (CI): 1.001 to 1.048]. Similarly, HF was associated with an increase in the risk of NAFLD (P < 0.001; OR = 1.117; 95% CI: 1.053 to 1.185). Conclusions Our findings reveal novel molecular signatures common to NAFLD and HF and confirm their bidirectional causality, highlighting the potential for targeted therapeutic interventions and prompting further investigation into their intricate relationship. |
| format | Article |
| id | doaj-art-c189ed0dcc764efea2079861fbd91cb6 |
| institution | OA Journals |
| issn | 2055-5822 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
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| series | ESC Heart Failure |
| spelling | doaj-art-c189ed0dcc764efea2079861fbd91cb62025-08-20T01:54:57ZengWileyESC Heart Failure2055-58222024-12-011164185420010.1002/ehf2.15019Non‐alcoholic fatty liver disease and heart failure: A comprehensive bioinformatics and Mendelian randomization analysisYayun Zhang0Lu Feng1Xin Guan2Zixiong Zhu3Yubin He4Xuewen Li5Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University Taiyuan ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University Taiyuan ChinaThird Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital Taiyuan ChinaThird Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital Taiyuan ChinaThird Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital Taiyuan ChinaShanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Third Hospital of Shanxi Medical University Taiyuan ChinaAbstract Aims Heart failure (HF) and non‐alcoholic fatty liver disease (NAFLD) are significant global health issues with a complex interrelationship. This study investigates their shared biomarkers and causal relationships using bioinformatics and Mendelian randomization (MR) approaches. Methods We analysed NAFLD and HF datasets from the Gene Expression Omnibus (GEO). The GSE126848 dataset included 57 liver biopsy samples [14 healthy individuals, 12 obese subjects, 15 NAFL patients and 16 non‐alcoholic steatohepatitis (NASH) patients]. The GSE24807 dataset comprised 12 NASH samples and 5 healthy controls. The GSE57338 dataset included 313 cardiac muscle samples [177 HF patients (95 ischaemic heart disease patients and 82 idiopathic dilated cardiomyopathy patients) and 136 healthy controls]. The GSE84796 dataset consisted of 10 end‐stage HF patients and 7 healthy hearts procured from organ donors. We identified differentially expressed genes (DEGs) and constructed a protein–protein interaction (PPI) network. Functional pathways were elucidated through enrichment analyses using Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG) and GeneMANIA annotation. Single nucleotide polymorphism (SNP) data for HF and NAFLD were sourced from genome‐wide association studies (GWAS). The HF dataset included 486 160 samples (14 262 experimental and 471 898 control), and the NAFLD dataset comprised 377 988 samples (4761 experimental and 373 227 control). MR analysis investigates the causal interrelations. Results Our analysis revealed 4032 DEGs from GSE126848 and 286 DEGs from GSE57338. The top 10 hub genes (CD163, VSIG4, CXCL10, FCER1G, FPR1, C1QB, CCR1, C1orf162, MRC1 and CD38) were significantly enriched in immune response, calcium ion concentration regulation and positive regulation of monocyte chemotaxis. CIBERSORT analysis indicated associations between these hub genes and natural killer (NK) cells and macrophages. Transcription factor (TF) target prediction for CD38, CXCL10 and CCR1 highlighted related TFs. A two‐sample MR analysis confirmed a bidirectional causal relationship between NAFLD and HF. The main method [inverse variance weighted (IVW)] demonstrated a significant positive causal relationship between NAFLD and HF [P = 0.037; odds ratio (OR) = 1.024; 95% confidence interval (CI): 1.001 to 1.048]. Similarly, HF was associated with an increase in the risk of NAFLD (P < 0.001; OR = 1.117; 95% CI: 1.053 to 1.185). Conclusions Our findings reveal novel molecular signatures common to NAFLD and HF and confirm their bidirectional causality, highlighting the potential for targeted therapeutic interventions and prompting further investigation into their intricate relationship.https://doi.org/10.1002/ehf2.15019co‐expressed geneGene Expression Omnibusheart failureMendelian randomizationnon‐alcoholic fatty liver disease |
| spellingShingle | Yayun Zhang Lu Feng Xin Guan Zixiong Zhu Yubin He Xuewen Li Non‐alcoholic fatty liver disease and heart failure: A comprehensive bioinformatics and Mendelian randomization analysis ESC Heart Failure co‐expressed gene Gene Expression Omnibus heart failure Mendelian randomization non‐alcoholic fatty liver disease |
| title | Non‐alcoholic fatty liver disease and heart failure: A comprehensive bioinformatics and Mendelian randomization analysis |
| title_full | Non‐alcoholic fatty liver disease and heart failure: A comprehensive bioinformatics and Mendelian randomization analysis |
| title_fullStr | Non‐alcoholic fatty liver disease and heart failure: A comprehensive bioinformatics and Mendelian randomization analysis |
| title_full_unstemmed | Non‐alcoholic fatty liver disease and heart failure: A comprehensive bioinformatics and Mendelian randomization analysis |
| title_short | Non‐alcoholic fatty liver disease and heart failure: A comprehensive bioinformatics and Mendelian randomization analysis |
| title_sort | non alcoholic fatty liver disease and heart failure a comprehensive bioinformatics and mendelian randomization analysis |
| topic | co‐expressed gene Gene Expression Omnibus heart failure Mendelian randomization non‐alcoholic fatty liver disease |
| url | https://doi.org/10.1002/ehf2.15019 |
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