Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods
Four preparations of crude polysaccharides were obtained from Cedrela sinensis using four methods of extraction, and finally precipitation with ethanol. Hot-water and processes assisted by ultrasound and enzymes (Shearzyme or Viscozyme) were carried out. The yield of polysaccharide fraction extracte...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2018-12-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://www.degruyter.com/view/j/pjfns.2018.68.issue-4/pjfns-2018-0007/pjfns-2018-0007.xml?format=INT |
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| author | Oh Min Hui Yoon Kyung Young |
| author_facet | Oh Min Hui Yoon Kyung Young |
| author_sort | Oh Min Hui |
| collection | DOAJ |
| description | Four preparations of crude polysaccharides were obtained from Cedrela sinensis using four methods of extraction, and finally precipitation with ethanol. Hot-water and processes assisted by ultrasound and enzymes (Shearzyme or Viscozyme) were carried out. The yield of polysaccharide fraction extracted with the enzyme-assisted method using Shearzyme (P-EAS) was the highest at 10.53%. All crude saccharides were composed of six monosaccharides including arabinose, galactose, glucose, mannose, rhamnose, and xylose. Crude polysaccharide fraction extracted by hot water showed the highest DPPH• and ABTS•+ scavenging activities as well as α-amylase inhibitory activity. All fractions showed higher retardation effects on glucose and bile acid absorption using the in vitro dialysis system compared to the control (without polysaccharide fraction); specifically, the glucose retardation index of P-EAS was similar to that of carboxymethylcellulose. Overall, crude polysaccharide fractions obtained from C. sinensis can be used as functional ingredients owing to their excellent functional properties. |
| format | Article |
| id | doaj-art-c17ea4ad2d864aecb212646588c41ae3 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2018-12-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-c17ea4ad2d864aecb212646588c41ae32025-02-02T18:52:28ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072018-12-0168432733410.1515/pjfns-2018-0007pjfns-2018-0007Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction MethodsOh Min Hui0Yoon Kyung Young1Department of Food and Nutrition, Yeungnam University, Gyeongsan, Gyeongbuk 38547, KoreaDepartment of Food and Nutrition, Yeungnam University, Gyeongsan, Gyeongbuk 38547, KoreaFour preparations of crude polysaccharides were obtained from Cedrela sinensis using four methods of extraction, and finally precipitation with ethanol. Hot-water and processes assisted by ultrasound and enzymes (Shearzyme or Viscozyme) were carried out. The yield of polysaccharide fraction extracted with the enzyme-assisted method using Shearzyme (P-EAS) was the highest at 10.53%. All crude saccharides were composed of six monosaccharides including arabinose, galactose, glucose, mannose, rhamnose, and xylose. Crude polysaccharide fraction extracted by hot water showed the highest DPPH• and ABTS•+ scavenging activities as well as α-amylase inhibitory activity. All fractions showed higher retardation effects on glucose and bile acid absorption using the in vitro dialysis system compared to the control (without polysaccharide fraction); specifically, the glucose retardation index of P-EAS was similar to that of carboxymethylcellulose. Overall, crude polysaccharide fractions obtained from C. sinensis can be used as functional ingredients owing to their excellent functional properties.http://www.degruyter.com/view/j/pjfns.2018.68.issue-4/pjfns-2018-0007/pjfns-2018-0007.xml?format=INTCedrela sinensisextraction methodpolysaccharidesantioxidant activitybiological activity |
| spellingShingle | Oh Min Hui Yoon Kyung Young Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods Polish Journal of Food and Nutrition Sciences Cedrela sinensis extraction method polysaccharides antioxidant activity biological activity |
| title | Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods |
| title_full | Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods |
| title_fullStr | Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods |
| title_full_unstemmed | Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods |
| title_short | Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods |
| title_sort | comparison of the biological activity of crude polysaccharide fractions obtained from cedrela sinensis using different extraction methods |
| topic | Cedrela sinensis extraction method polysaccharides antioxidant activity biological activity |
| url | http://www.degruyter.com/view/j/pjfns.2018.68.issue-4/pjfns-2018-0007/pjfns-2018-0007.xml?format=INT |
| work_keys_str_mv | AT ohminhui comparisonofthebiologicalactivityofcrudepolysaccharidefractionsobtainedfromcedrelasinensisusingdifferentextractionmethods AT yoonkyungyoung comparisonofthebiologicalactivityofcrudepolysaccharidefractionsobtainedfromcedrelasinensisusingdifferentextractionmethods |