Effects of different sodium concentrations in fluids on brain, lung, and kidney in experimental ischemic stroke
Abstract Fluid administration is a key component in the management of acute ischemic stroke (AIS). However, the effects of different sodium concentrations in resuscitation fluids, particularly on distal organ function, remain controversial. This study compared the impact of four commonly used fluids...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-12491-9 |
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| author | Camila M. Bessa Adriana L. Vilardo Diogo B. Peruchetti Pedro H. L. Conceição Celso Caruso-Neves Vera L. Capelozzi Denise Battaglini Chiara Robba Paolo Pelosi Manu L. N. G. Malbrain Patricia R. M. Rocco Pedro L. Silva Cynthia S. Samary |
| author_facet | Camila M. Bessa Adriana L. Vilardo Diogo B. Peruchetti Pedro H. L. Conceição Celso Caruso-Neves Vera L. Capelozzi Denise Battaglini Chiara Robba Paolo Pelosi Manu L. N. G. Malbrain Patricia R. M. Rocco Pedro L. Silva Cynthia S. Samary |
| author_sort | Camila M. Bessa |
| collection | DOAJ |
| description | Abstract Fluid administration is a key component in the management of acute ischemic stroke (AIS). However, the effects of different sodium concentrations in resuscitation fluids, particularly on distal organ function, remain controversial. This study compared the impact of four commonly used fluids—0.9% isotonic saline (ISO), 0.45% hypotonic saline (HYPO), 1.5% hypertonic saline (HYPER), and 5% glucose (GLUCO)—on perilesional brain tissue, lungs, and kidneys following AIS. AIS was induced in 28 male Wistar rats. Three hours after stroke induction, animals were randomized to receive one of the four fluids. In the brain, the ISO group showed significantly higher expression of versican and hyaluronan compared to the HYPER group (p = 0.022 and p = 0.018, respectively). Conversely, the HYPER group exhibited significantly elevated levels of interleukin-1β (IL-1β), vascular cell adhesion molecule-1 (VCAM-1), and zonula occludens-1 (ZO-1) compared to the GLUCO group (p = 0.01, p = 0.02, and p = 0.006, respectively). In the lungs, the ISO group demonstrated less alveolar collapse and pulmonary edema compared to the HYPER and HYPO groups (p = 0.01 and p = 0.007, respectively). In the kidneys, both the ISO and HYPO groups showed significantly less brush-border injury than the HYPER group (p = 0.007 and p = 0.032, respectively). Furthermore, blood chloride levels declined over time in the ISO group compared to the others. In conclusion, isotonic fluid administration resulted in the least amount of injury to the brain, lungs, and kidneys in this experimental model of AIS, supporting its use as a preferred resuscitation strategy in the acute phase. |
| format | Article |
| id | doaj-art-c177be804ca04e7cbb42489c9d791284 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
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| spelling | doaj-art-c177be804ca04e7cbb42489c9d7912842025-08-20T03:05:22ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-12491-9Effects of different sodium concentrations in fluids on brain, lung, and kidney in experimental ischemic strokeCamila M. Bessa0Adriana L. Vilardo1Diogo B. Peruchetti2Pedro H. L. Conceição3Celso Caruso-Neves4Vera L. Capelozzi5Denise Battaglini6Chiara Robba7Paolo Pelosi8Manu L. N. G. Malbrain9Patricia R. M. Rocco10Pedro L. Silva11Cynthia S. Samary12Laboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de JaneiroLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de JaneiroDepartment of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas GeraisLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de JaneiroLaboratory of Biochemistry and Cell Signaling, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de JaneiroDepartment of Pathology, Faculty of Medicine, University of São PauloDepartment of Surgical Sciences and Integrated Diagnostics (DISC), University of GenoaDepartment of Surgical Sciences and Integrated Diagnostics (DISC), University of GenoaDepartment of Surgical Sciences and Integrated Diagnostics (DISC), University of GenoaFirst Department of Anesthesiology and Intensive Therapy, Medical University of LublinLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de JaneiroLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de JaneiroLaboratory of Pulmonary Investigation, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de JaneiroAbstract Fluid administration is a key component in the management of acute ischemic stroke (AIS). However, the effects of different sodium concentrations in resuscitation fluids, particularly on distal organ function, remain controversial. This study compared the impact of four commonly used fluids—0.9% isotonic saline (ISO), 0.45% hypotonic saline (HYPO), 1.5% hypertonic saline (HYPER), and 5% glucose (GLUCO)—on perilesional brain tissue, lungs, and kidneys following AIS. AIS was induced in 28 male Wistar rats. Three hours after stroke induction, animals were randomized to receive one of the four fluids. In the brain, the ISO group showed significantly higher expression of versican and hyaluronan compared to the HYPER group (p = 0.022 and p = 0.018, respectively). Conversely, the HYPER group exhibited significantly elevated levels of interleukin-1β (IL-1β), vascular cell adhesion molecule-1 (VCAM-1), and zonula occludens-1 (ZO-1) compared to the GLUCO group (p = 0.01, p = 0.02, and p = 0.006, respectively). In the lungs, the ISO group demonstrated less alveolar collapse and pulmonary edema compared to the HYPER and HYPO groups (p = 0.01 and p = 0.007, respectively). In the kidneys, both the ISO and HYPO groups showed significantly less brush-border injury than the HYPER group (p = 0.007 and p = 0.032, respectively). Furthermore, blood chloride levels declined over time in the ISO group compared to the others. In conclusion, isotonic fluid administration resulted in the least amount of injury to the brain, lungs, and kidneys in this experimental model of AIS, supporting its use as a preferred resuscitation strategy in the acute phase.https://doi.org/10.1038/s41598-025-12491-9Acute ischemic strokeInflammationBrain damageSodium concentrationFluids |
| spellingShingle | Camila M. Bessa Adriana L. Vilardo Diogo B. Peruchetti Pedro H. L. Conceição Celso Caruso-Neves Vera L. Capelozzi Denise Battaglini Chiara Robba Paolo Pelosi Manu L. N. G. Malbrain Patricia R. M. Rocco Pedro L. Silva Cynthia S. Samary Effects of different sodium concentrations in fluids on brain, lung, and kidney in experimental ischemic stroke Scientific Reports Acute ischemic stroke Inflammation Brain damage Sodium concentration Fluids |
| title | Effects of different sodium concentrations in fluids on brain, lung, and kidney in experimental ischemic stroke |
| title_full | Effects of different sodium concentrations in fluids on brain, lung, and kidney in experimental ischemic stroke |
| title_fullStr | Effects of different sodium concentrations in fluids on brain, lung, and kidney in experimental ischemic stroke |
| title_full_unstemmed | Effects of different sodium concentrations in fluids on brain, lung, and kidney in experimental ischemic stroke |
| title_short | Effects of different sodium concentrations in fluids on brain, lung, and kidney in experimental ischemic stroke |
| title_sort | effects of different sodium concentrations in fluids on brain lung and kidney in experimental ischemic stroke |
| topic | Acute ischemic stroke Inflammation Brain damage Sodium concentration Fluids |
| url | https://doi.org/10.1038/s41598-025-12491-9 |
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