<i>CYP6B</i> Subtype Expression Fluctuates in the Great Mormon, <i>Papilio memnon</i>, with Changes in the Components of the Host Plants
Cytochrome P-450 (CYP) is one of the metabolic enzymes which is conserved among organisms to metabolise xenobiotics. The metabolic role and transcriptomic profiles of the CYP6B subfamily have been studied in some Papilionidae insects. However, the role of <i>CYP6B</i>s expressed in Rutac...
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2025-02-01
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| author | Miho Nakano Takuma Sakamoto Yoshikazu Kitano Hidemasa Bono Hiroko Tabunoki |
| author_facet | Miho Nakano Takuma Sakamoto Yoshikazu Kitano Hidemasa Bono Hiroko Tabunoki |
| author_sort | Miho Nakano |
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| description | Cytochrome P-450 (CYP) is one of the metabolic enzymes which is conserved among organisms to metabolise xenobiotics. The metabolic role and transcriptomic profiles of the CYP6B subfamily have been studied in some Papilionidae insects. However, the role of <i>CYP6B</i>s expressed in Rutaceae plant-fed swallowtails has yet to be fully examined. Here, the expression profile of <i>CYP6B</i> in the Great Mormon (<i>Papilio memnon</i>) larvae, which prefer Citrus plants to the Rutaceae plant, was investigated by RNA sequencing analysis and real-time quantitative PCR when feeding different kinds of Citrus plants. We found that six kinds of <i>CYP6B</i> subtype transcripts were expressed in the larval fat body and midgut. Then, we focused on <i>CYP6B2</i>, <i>CYP6B5</i>, and <i>CYP6B6</i> transcripts and examined their mRNA expression in the larvae fed on different plants. These <i>CYP6B</i> mRNA expressions were changed in the larval fat body and the midgut by changing the host plants. The presence of chemicals specific to each host plant was confirmed when the host plant components were examined by database, literature, and thin-layer chromatography. Our study suggests that phytochemicals in the host plant affect <i>CYP6B</i> subtypes mRNA expression in <i>P. memnon</i> larval fat body and midgut, and CYP6B subtypes may relate to metabolise the phytochemicals in the host plants. |
| format | Article |
| id | doaj-art-c176ee1f067d4f409602b731cc91b2f6 |
| institution | DOAJ |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-c176ee1f067d4f409602b731cc91b2f62025-08-20T03:12:02ZengMDPI AGInsects2075-44502025-02-0116215910.3390/insects16020159<i>CYP6B</i> Subtype Expression Fluctuates in the Great Mormon, <i>Papilio memnon</i>, with Changes in the Components of the Host PlantsMiho Nakano0Takuma Sakamoto1Yoshikazu Kitano2Hidemasa Bono3Hiroko Tabunoki4Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Tokyo 183-8509, JapanDepartment of Science of Biological Production, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, JapanDepartment of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo 183-8509, JapanLaboratory of Bio-DX, Genome Editing Innovation Center, Hiroshima University, Hiroshima 739-0046, JapanCooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Tokyo 183-8509, JapanCytochrome P-450 (CYP) is one of the metabolic enzymes which is conserved among organisms to metabolise xenobiotics. The metabolic role and transcriptomic profiles of the CYP6B subfamily have been studied in some Papilionidae insects. However, the role of <i>CYP6B</i>s expressed in Rutaceae plant-fed swallowtails has yet to be fully examined. Here, the expression profile of <i>CYP6B</i> in the Great Mormon (<i>Papilio memnon</i>) larvae, which prefer Citrus plants to the Rutaceae plant, was investigated by RNA sequencing analysis and real-time quantitative PCR when feeding different kinds of Citrus plants. We found that six kinds of <i>CYP6B</i> subtype transcripts were expressed in the larval fat body and midgut. Then, we focused on <i>CYP6B2</i>, <i>CYP6B5</i>, and <i>CYP6B6</i> transcripts and examined their mRNA expression in the larvae fed on different plants. These <i>CYP6B</i> mRNA expressions were changed in the larval fat body and the midgut by changing the host plants. The presence of chemicals specific to each host plant was confirmed when the host plant components were examined by database, literature, and thin-layer chromatography. Our study suggests that phytochemicals in the host plant affect <i>CYP6B</i> subtypes mRNA expression in <i>P. memnon</i> larval fat body and midgut, and CYP6B subtypes may relate to metabolise the phytochemicals in the host plants.https://www.mdpi.com/2075-4450/16/2/159cytochrome P450CYP<i>CYP6B</i>Papilionidae<i>Papilio memnon</i>insect metabolism |
| spellingShingle | Miho Nakano Takuma Sakamoto Yoshikazu Kitano Hidemasa Bono Hiroko Tabunoki <i>CYP6B</i> Subtype Expression Fluctuates in the Great Mormon, <i>Papilio memnon</i>, with Changes in the Components of the Host Plants Insects cytochrome P450 CYP <i>CYP6B</i> Papilionidae <i>Papilio memnon</i> insect metabolism |
| title | <i>CYP6B</i> Subtype Expression Fluctuates in the Great Mormon, <i>Papilio memnon</i>, with Changes in the Components of the Host Plants |
| title_full | <i>CYP6B</i> Subtype Expression Fluctuates in the Great Mormon, <i>Papilio memnon</i>, with Changes in the Components of the Host Plants |
| title_fullStr | <i>CYP6B</i> Subtype Expression Fluctuates in the Great Mormon, <i>Papilio memnon</i>, with Changes in the Components of the Host Plants |
| title_full_unstemmed | <i>CYP6B</i> Subtype Expression Fluctuates in the Great Mormon, <i>Papilio memnon</i>, with Changes in the Components of the Host Plants |
| title_short | <i>CYP6B</i> Subtype Expression Fluctuates in the Great Mormon, <i>Papilio memnon</i>, with Changes in the Components of the Host Plants |
| title_sort | i cyp6b i subtype expression fluctuates in the great mormon i papilio memnon i with changes in the components of the host plants |
| topic | cytochrome P450 CYP <i>CYP6B</i> Papilionidae <i>Papilio memnon</i> insect metabolism |
| url | https://www.mdpi.com/2075-4450/16/2/159 |
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