Synthesis, Characterization, and Biological Activity of a Novel <i>SA-g-p(AAc-co-AM)/ZnO NP</i> Hydrogel Composite

This research investigated the preparation and efficacy of a <i>SA-g-p(AAc-co-AM)/ZnO</i> hydrogel composite with enhanced biological activity. The hydrogel was synthesized using the sodium alginate biopolymer through the co-polymerization method. Our findings indicate that introducing z...

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Main Authors: Aseel M. Aljeboree, Hadeel K. Albdairi, Mohammed Abed Jawad, Sarah A. Hamood, Firas H. Abdulrazzak, Ayad F. Alkaim
Format: Article
Language:English
Published: MDPI AG 2024-01-01
Series:Engineering Proceedings
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Online Access:https://www.mdpi.com/2673-4591/59/1/192
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author Aseel M. Aljeboree
Hadeel K. Albdairi
Mohammed Abed Jawad
Sarah A. Hamood
Firas H. Abdulrazzak
Ayad F. Alkaim
author_facet Aseel M. Aljeboree
Hadeel K. Albdairi
Mohammed Abed Jawad
Sarah A. Hamood
Firas H. Abdulrazzak
Ayad F. Alkaim
author_sort Aseel M. Aljeboree
collection DOAJ
description This research investigated the preparation and efficacy of a <i>SA-g-p(AAc-co-AM)/ZnO</i> hydrogel composite with enhanced biological activity. The hydrogel was synthesized using the sodium alginate biopolymer through the co-polymerization method. Our findings indicate that introducing zinc oxide nanoparticles to the hydrogel amplified its biological activity. The disc diffusion technique was applied to evaluate the antimicrobial properties against two Gram-positive bacteria isolates (<i>Staphylococcus aureus, Streptococcus epigenetics</i>) and two Gram-negative bacteria (<i>E.coli, Klebsiella</i> spp.). The antimicrobial activities of three surfaces—ZnO, <i>SA-g-p(AAc-co-AM)</i> hydrogel, and <i>SA-g-p(AAc-co-AM)/ZnO</i> hydrogel composite—were assessed. Characterization of these prepared surfaces was executed using FE-SEM and EDX. The results highlighted that the <i>ZnO NPs</i> exhibited minimal antibacterial activity against both types of bacteria. Conversely, the <i>SA-g-p(AAc-co-AM)/ZnO</i> hydrogel composite demonstrated heightened antibacterial effects against <i>Staphylococcus aureus</i> (30 mm) and <i>Streptococcus epigenetics</i> (25 mm). The Gram-negative bacteria, <i>E.coli</i> and <i>Klebsiella</i> spp., recorded inhibition zones of 13 mm and 12 mm, respectively. The <i>SA-g-p(AAc-co-AM)</i> hydrogel showed diminished antibacterial activity relative to the composite, attributed to the absence of zinc oxide. Overall, the isolated effect of zinc oxide nanoparticles indicated a minimal antibacterial influence on all Gram-positive and Gram-negative bacteria strains.
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spelling doaj-art-c1605b7ae05e46e8a5109cbca06e5cd02025-08-20T02:42:45ZengMDPI AGEngineering Proceedings2673-45912024-01-0159119210.3390/engproc2023059192Synthesis, Characterization, and Biological Activity of a Novel <i>SA-g-p(AAc-co-AM)/ZnO NP</i> Hydrogel CompositeAseel M. Aljeboree0Hadeel K. Albdairi1Mohammed Abed Jawad2Sarah A. Hamood3Firas H. Abdulrazzak4Ayad F. Alkaim5Department of Chemistry, College of Sciences for Girls, University of Babylon, Hilla 5001, IraqBabylon Police Command, Ministry of Interior, Hilla 5001, IraqAnesthesia Techniques Department, Al-Nisour University College, Baghdad 10011, IraqDepartment of Medical Laboratories Technology, Al-Esraa University College, Baghdad 10011, IraqForensic Evidence Department, College of Science, Al-Karkh University of Science, Baghdad 10081, IraqDepartment of Chemistry, College of Sciences for Girls, University of Babylon, Hilla 5001, IraqThis research investigated the preparation and efficacy of a <i>SA-g-p(AAc-co-AM)/ZnO</i> hydrogel composite with enhanced biological activity. The hydrogel was synthesized using the sodium alginate biopolymer through the co-polymerization method. Our findings indicate that introducing zinc oxide nanoparticles to the hydrogel amplified its biological activity. The disc diffusion technique was applied to evaluate the antimicrobial properties against two Gram-positive bacteria isolates (<i>Staphylococcus aureus, Streptococcus epigenetics</i>) and two Gram-negative bacteria (<i>E.coli, Klebsiella</i> spp.). The antimicrobial activities of three surfaces—ZnO, <i>SA-g-p(AAc-co-AM)</i> hydrogel, and <i>SA-g-p(AAc-co-AM)/ZnO</i> hydrogel composite—were assessed. Characterization of these prepared surfaces was executed using FE-SEM and EDX. The results highlighted that the <i>ZnO NPs</i> exhibited minimal antibacterial activity against both types of bacteria. Conversely, the <i>SA-g-p(AAc-co-AM)/ZnO</i> hydrogel composite demonstrated heightened antibacterial effects against <i>Staphylococcus aureus</i> (30 mm) and <i>Streptococcus epigenetics</i> (25 mm). The Gram-negative bacteria, <i>E.coli</i> and <i>Klebsiella</i> spp., recorded inhibition zones of 13 mm and 12 mm, respectively. The <i>SA-g-p(AAc-co-AM)</i> hydrogel showed diminished antibacterial activity relative to the composite, attributed to the absence of zinc oxide. Overall, the isolated effect of zinc oxide nanoparticles indicated a minimal antibacterial influence on all Gram-positive and Gram-negative bacteria strains.https://www.mdpi.com/2673-4591/59/1/192hydrogelsodium alginatezinc oxideantibacterialGram-positiveGram-negative
spellingShingle Aseel M. Aljeboree
Hadeel K. Albdairi
Mohammed Abed Jawad
Sarah A. Hamood
Firas H. Abdulrazzak
Ayad F. Alkaim
Synthesis, Characterization, and Biological Activity of a Novel <i>SA-g-p(AAc-co-AM)/ZnO NP</i> Hydrogel Composite
Engineering Proceedings
hydrogel
sodium alginate
zinc oxide
antibacterial
Gram-positive
Gram-negative
title Synthesis, Characterization, and Biological Activity of a Novel <i>SA-g-p(AAc-co-AM)/ZnO NP</i> Hydrogel Composite
title_full Synthesis, Characterization, and Biological Activity of a Novel <i>SA-g-p(AAc-co-AM)/ZnO NP</i> Hydrogel Composite
title_fullStr Synthesis, Characterization, and Biological Activity of a Novel <i>SA-g-p(AAc-co-AM)/ZnO NP</i> Hydrogel Composite
title_full_unstemmed Synthesis, Characterization, and Biological Activity of a Novel <i>SA-g-p(AAc-co-AM)/ZnO NP</i> Hydrogel Composite
title_short Synthesis, Characterization, and Biological Activity of a Novel <i>SA-g-p(AAc-co-AM)/ZnO NP</i> Hydrogel Composite
title_sort synthesis characterization and biological activity of a novel i sa g p aac co am zno np i hydrogel composite
topic hydrogel
sodium alginate
zinc oxide
antibacterial
Gram-positive
Gram-negative
url https://www.mdpi.com/2673-4591/59/1/192
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