Expression Profiling and Function Analysis Identified New Cumulus Cells-Expressed Genes and miRNAs Predictive of Oocyte Developmental Potential
Although prior studies have identified cumulus cells (CCs)-expressed genes and miRNAs that regulate cumulus expansion and/or CC apoptosis and may serve as markers for selecting competent oocytes and embryos, there remains an urgent need to identify CCs-expressed genes and miRNAs whose expression lev...
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2025-05-01
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| author | Min Zhang Meng-Meng Wang Fa-Li Zhang Nan He Ming-Jiu Luo Shuai Gong Fu-Yin Fu Hong-Jie Yuan Jie Zhang Jing-He Tan |
| author_facet | Min Zhang Meng-Meng Wang Fa-Li Zhang Nan He Ming-Jiu Luo Shuai Gong Fu-Yin Fu Hong-Jie Yuan Jie Zhang Jing-He Tan |
| author_sort | Min Zhang |
| collection | DOAJ |
| description | Although prior studies have identified cumulus cells (CCs)-expressed genes and miRNAs that regulate cumulus expansion and/or CC apoptosis and may serve as markers for selecting competent oocytes and embryos, there remains an urgent need to identify CCs-expressed genes and miRNAs whose expression levels are directly correlated with oocyte developmental potential (DP). In this study, we first established CC models from mouse cumulus-oocyte complexes (COCs) that exhibited significantly different DP following in vitro or in vivo maturation. Subsequently, we performed mRNA/miRNA sequencing and functional analyses using these in vitro and in vivo CC models. We identified and validated Spp1, Fn1, Sdc1, and Ngf as DP-beneficial genes; Fos and Jun as DP-detrimental genes; and miR-7686-5p, miR-133a-3p, novel-miR-239, novel-miR-193, and miR-339-5p as DP-detrimental miRNAs. Finally, by employing a well-in-well activation/embryo culture system that enables tracking the COC origin of CCs and embryos, we further validated Spp1 and Ngf as DP-beneficial genes, Jun as the DP-detrimental gene, and miR-7686-5p, novel-miR-239, and miR-339-5p as DP-detrimental miRNAs. In conclusion, we identified and validated new sets of CCs-expressed genes and miRNAs whose expression levels were directly correlated with oocyte DP. These newly identified genes and miRNAs may serve as potential biomarkers for selecting competent oocytes and embryos. |
| format | Article |
| id | doaj-art-c15e4e0758e740978d81176d391691b8 |
| institution | Kabale University |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Cells |
| spelling | doaj-art-c15e4e0758e740978d81176d391691b82025-08-20T03:46:48ZengMDPI AGCells2073-44092025-05-01141179110.3390/cells14110791Expression Profiling and Function Analysis Identified New Cumulus Cells-Expressed Genes and miRNAs Predictive of Oocyte Developmental PotentialMin Zhang0Meng-Meng Wang1Fa-Li Zhang2Nan He3Ming-Jiu Luo4Shuai Gong5Fu-Yin Fu6Hong-Jie Yuan7Jie Zhang8Jing-He Tan9College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, ChinaCollege of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City 271018, ChinaAlthough prior studies have identified cumulus cells (CCs)-expressed genes and miRNAs that regulate cumulus expansion and/or CC apoptosis and may serve as markers for selecting competent oocytes and embryos, there remains an urgent need to identify CCs-expressed genes and miRNAs whose expression levels are directly correlated with oocyte developmental potential (DP). In this study, we first established CC models from mouse cumulus-oocyte complexes (COCs) that exhibited significantly different DP following in vitro or in vivo maturation. Subsequently, we performed mRNA/miRNA sequencing and functional analyses using these in vitro and in vivo CC models. We identified and validated Spp1, Fn1, Sdc1, and Ngf as DP-beneficial genes; Fos and Jun as DP-detrimental genes; and miR-7686-5p, miR-133a-3p, novel-miR-239, novel-miR-193, and miR-339-5p as DP-detrimental miRNAs. Finally, by employing a well-in-well activation/embryo culture system that enables tracking the COC origin of CCs and embryos, we further validated Spp1 and Ngf as DP-beneficial genes, Jun as the DP-detrimental gene, and miR-7686-5p, novel-miR-239, and miR-339-5p as DP-detrimental miRNAs. In conclusion, we identified and validated new sets of CCs-expressed genes and miRNAs whose expression levels were directly correlated with oocyte DP. These newly identified genes and miRNAs may serve as potential biomarkers for selecting competent oocytes and embryos.https://www.mdpi.com/2073-4409/14/11/791cumulus cellsdevelopmental potentialgenesmiRNAsoocytes |
| spellingShingle | Min Zhang Meng-Meng Wang Fa-Li Zhang Nan He Ming-Jiu Luo Shuai Gong Fu-Yin Fu Hong-Jie Yuan Jie Zhang Jing-He Tan Expression Profiling and Function Analysis Identified New Cumulus Cells-Expressed Genes and miRNAs Predictive of Oocyte Developmental Potential Cells cumulus cells developmental potential genes miRNAs oocytes |
| title | Expression Profiling and Function Analysis Identified New Cumulus Cells-Expressed Genes and miRNAs Predictive of Oocyte Developmental Potential |
| title_full | Expression Profiling and Function Analysis Identified New Cumulus Cells-Expressed Genes and miRNAs Predictive of Oocyte Developmental Potential |
| title_fullStr | Expression Profiling and Function Analysis Identified New Cumulus Cells-Expressed Genes and miRNAs Predictive of Oocyte Developmental Potential |
| title_full_unstemmed | Expression Profiling and Function Analysis Identified New Cumulus Cells-Expressed Genes and miRNAs Predictive of Oocyte Developmental Potential |
| title_short | Expression Profiling and Function Analysis Identified New Cumulus Cells-Expressed Genes and miRNAs Predictive of Oocyte Developmental Potential |
| title_sort | expression profiling and function analysis identified new cumulus cells expressed genes and mirnas predictive of oocyte developmental potential |
| topic | cumulus cells developmental potential genes miRNAs oocytes |
| url | https://www.mdpi.com/2073-4409/14/11/791 |
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