Impact of Microbiota Diversity on Inflammatory Bowel Disease
Inflammatory bowel disease (IBD) is a chronic condition that includes two main types, Crohn’s disease (CD) and ulcerative colitis (UC), involving inflammation of the gastrointestinal (GI) tract. The exact cause of IBD is unknown but could be a combination of genetic, environmental, and immune system...
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MDPI AG
2025-03-01
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| author | Ashwag J. Alzahrani Basma M. Al-Hebshi Zolfekar A. Yahia Effat A. Al-Judaibi Khloud H. Alsaadi Awatif A. Al-Judaibi |
| author_facet | Ashwag J. Alzahrani Basma M. Al-Hebshi Zolfekar A. Yahia Effat A. Al-Judaibi Khloud H. Alsaadi Awatif A. Al-Judaibi |
| author_sort | Ashwag J. Alzahrani |
| collection | DOAJ |
| description | Inflammatory bowel disease (IBD) is a chronic condition that includes two main types, Crohn’s disease (CD) and ulcerative colitis (UC), involving inflammation of the gastrointestinal (GI) tract. The exact cause of IBD is unknown but could be a combination of genetic, environmental, and immune system factors. This study investigated the impact of IBD on microbiota diversity by evaluating the differences in microbial composition and the microbiota of a control group (A) of healthy individuals and a group (B) of IBD patients. Sixty biopsies were collected from participants recruited from hospitals in Makkah, Saudi Arabia. Biopsy specimens were taken during colonoscopy examination, and bacterial identification was performed by extracting ribosomal DNA from sigmoid colon biopsies using a DNeasy Blood & Tissue Kit. Metagenomics and bioinformatics analyses were then conducted to analyze and compare the microbiota in the two groups. The results showed that the varieties of core microbiome species were 3.81% greater in the IBD patients than in the members of the control group. Furthermore, the differences between the groups were significantly greater than the variations within each group. Differences between the two groups were detected in the relative abundance of <i>Clostridium nexile</i>, <i>Ruminococcus gnavus</i>, <i>Ruminococcus faecis</i>, and <i>Escherichia coli</i>. These results indicate that microbiota could play a role in the pathogenesis of IBD and suggest that microbial diversity can serve as a biomarker for diagnosing the disease and monitoring its progression. |
| format | Article |
| id | doaj-art-c15a0cf5f96a4faca1d249e1b27aa407 |
| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-c15a0cf5f96a4faca1d249e1b27aa4072025-08-20T02:18:14ZengMDPI AGMicroorganisms2076-26072025-03-0113471010.3390/microorganisms13040710Impact of Microbiota Diversity on Inflammatory Bowel DiseaseAshwag J. Alzahrani0Basma M. Al-Hebshi1Zolfekar A. Yahia2Effat A. Al-Judaibi3Khloud H. Alsaadi4Awatif A. Al-Judaibi5Department of Biological Sciences, Microbiology Section, College of Science, University of Jeddah, Jeddah 21959, Saudi ArabiaDepartment of Biological Sciences, Microbiology Section, College of Science, University of Jeddah, Jeddah 21959, Saudi ArabiaDepartment of Internal Medicine, Al Noor Specialist Hospital, Ministry of Health, Makkah 24242, Saudi ArabiaDepartment of Biological Sciences, Microbiology Section, College of Science, University of Jeddah, Jeddah 21959, Saudi ArabiaDepartment of Biological Sciences, Microbiology Section, College of Science, University of Jeddah, Jeddah 21959, Saudi ArabiaDepartment of Biological Sciences, Microbiology Section, College of Science, University of Jeddah, Jeddah 21959, Saudi ArabiaInflammatory bowel disease (IBD) is a chronic condition that includes two main types, Crohn’s disease (CD) and ulcerative colitis (UC), involving inflammation of the gastrointestinal (GI) tract. The exact cause of IBD is unknown but could be a combination of genetic, environmental, and immune system factors. This study investigated the impact of IBD on microbiota diversity by evaluating the differences in microbial composition and the microbiota of a control group (A) of healthy individuals and a group (B) of IBD patients. Sixty biopsies were collected from participants recruited from hospitals in Makkah, Saudi Arabia. Biopsy specimens were taken during colonoscopy examination, and bacterial identification was performed by extracting ribosomal DNA from sigmoid colon biopsies using a DNeasy Blood & Tissue Kit. Metagenomics and bioinformatics analyses were then conducted to analyze and compare the microbiota in the two groups. The results showed that the varieties of core microbiome species were 3.81% greater in the IBD patients than in the members of the control group. Furthermore, the differences between the groups were significantly greater than the variations within each group. Differences between the two groups were detected in the relative abundance of <i>Clostridium nexile</i>, <i>Ruminococcus gnavus</i>, <i>Ruminococcus faecis</i>, and <i>Escherichia coli</i>. These results indicate that microbiota could play a role in the pathogenesis of IBD and suggest that microbial diversity can serve as a biomarker for diagnosing the disease and monitoring its progression.https://www.mdpi.com/2076-2607/13/4/710inflammatory bowel diseaseCrohn’s diseaseulcerative colitismicrobial diversityintestinal microbiotabacteria |
| spellingShingle | Ashwag J. Alzahrani Basma M. Al-Hebshi Zolfekar A. Yahia Effat A. Al-Judaibi Khloud H. Alsaadi Awatif A. Al-Judaibi Impact of Microbiota Diversity on Inflammatory Bowel Disease Microorganisms inflammatory bowel disease Crohn’s disease ulcerative colitis microbial diversity intestinal microbiota bacteria |
| title | Impact of Microbiota Diversity on Inflammatory Bowel Disease |
| title_full | Impact of Microbiota Diversity on Inflammatory Bowel Disease |
| title_fullStr | Impact of Microbiota Diversity on Inflammatory Bowel Disease |
| title_full_unstemmed | Impact of Microbiota Diversity on Inflammatory Bowel Disease |
| title_short | Impact of Microbiota Diversity on Inflammatory Bowel Disease |
| title_sort | impact of microbiota diversity on inflammatory bowel disease |
| topic | inflammatory bowel disease Crohn’s disease ulcerative colitis microbial diversity intestinal microbiota bacteria |
| url | https://www.mdpi.com/2076-2607/13/4/710 |
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