Peptidylprolyl Isomerase A (PPIA) as a Preferred Internal Control Over GAPDH and β-Actin in Quantitative RNA Analyses
A good internal control is critical in all quantitative analyses of gene expression. Levels of β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and peptidylprolyl isomerase A (PPIA) were analyzed in 78 samples (data obtained from our laboratory and from a publicly available database at htt...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2002-04-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/02324st03 |
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| author | F. Feroze-Merzoug I.M. Berquin J. Dey Y.Q. Chen |
| author_facet | F. Feroze-Merzoug I.M. Berquin J. Dey Y.Q. Chen |
| author_sort | F. Feroze-Merzoug |
| collection | DOAJ |
| description | A good internal control is critical in all quantitative analyses of gene expression. Levels of β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and peptidylprolyl isomerase A (PPIA) were analyzed in 78 samples (data obtained from our laboratory and from a publicly available database at http://www.ncbi.nlm.nih.gov/SAGE/). These libraries included cell lines and tissues from brain, breast, colon, kidney, ovary, pancreas, prostate, skin, and vascular origin. The level of PPIA mRNA is the most constant among the three genes. Hence, our study suggests that PPIA is a better internal control than β-actin or GAPDH, the two most commonly used internal controls. |
| format | Article |
| id | doaj-art-c14c2d261bf34df381b2634b537689c9 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2002-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-c14c2d261bf34df381b2634b537689c92025-08-20T02:25:59ZengTaylor & Francis GroupBioTechniques0736-62051940-98182002-04-0132477678210.2144/02324st03Peptidylprolyl Isomerase A (PPIA) as a Preferred Internal Control Over GAPDH and β-Actin in Quantitative RNA AnalysesF. Feroze-Merzoug0I.M. Berquin1J. Dey2Y.Q. Chen31Wayne State University, Detroit, MI, USA1Wayne State University, Detroit, MI, USA1Wayne State University, Detroit, MI, USA1Wayne State University, Detroit, MI, USAA good internal control is critical in all quantitative analyses of gene expression. Levels of β-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and peptidylprolyl isomerase A (PPIA) were analyzed in 78 samples (data obtained from our laboratory and from a publicly available database at http://www.ncbi.nlm.nih.gov/SAGE/). These libraries included cell lines and tissues from brain, breast, colon, kidney, ovary, pancreas, prostate, skin, and vascular origin. The level of PPIA mRNA is the most constant among the three genes. Hence, our study suggests that PPIA is a better internal control than β-actin or GAPDH, the two most commonly used internal controls.https://www.future-science.com/doi/10.2144/02324st03 |
| spellingShingle | F. Feroze-Merzoug I.M. Berquin J. Dey Y.Q. Chen Peptidylprolyl Isomerase A (PPIA) as a Preferred Internal Control Over GAPDH and β-Actin in Quantitative RNA Analyses BioTechniques |
| title | Peptidylprolyl Isomerase A (PPIA) as a Preferred Internal Control Over GAPDH and β-Actin in Quantitative RNA Analyses |
| title_full | Peptidylprolyl Isomerase A (PPIA) as a Preferred Internal Control Over GAPDH and β-Actin in Quantitative RNA Analyses |
| title_fullStr | Peptidylprolyl Isomerase A (PPIA) as a Preferred Internal Control Over GAPDH and β-Actin in Quantitative RNA Analyses |
| title_full_unstemmed | Peptidylprolyl Isomerase A (PPIA) as a Preferred Internal Control Over GAPDH and β-Actin in Quantitative RNA Analyses |
| title_short | Peptidylprolyl Isomerase A (PPIA) as a Preferred Internal Control Over GAPDH and β-Actin in Quantitative RNA Analyses |
| title_sort | peptidylprolyl isomerase a ppia as a preferred internal control over gapdh and β actin in quantitative rna analyses |
| url | https://www.future-science.com/doi/10.2144/02324st03 |
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