Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection
Although microRNAs (miRNAs) have emerged as increasingly important target analytes, their biorecognition remains challenging due to their small size, high sequence homology, and low abundance in clinical samples. Nanospheres and microspheres have also gained increasing attention in biosensor applica...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Advances in Polymer Technology |
| Online Access: | http://dx.doi.org/10.1155/2020/7310657 |
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| author | Samira Hosseini Patricia Vázquez-Villegas Richard C. Willson Marco Rito-Palomares Margarita Sanchez-Dominguez Leo H. Koole Marc J. Madou Sergio O. Martínez-Chapa |
| author_facet | Samira Hosseini Patricia Vázquez-Villegas Richard C. Willson Marco Rito-Palomares Margarita Sanchez-Dominguez Leo H. Koole Marc J. Madou Sergio O. Martínez-Chapa |
| author_sort | Samira Hosseini |
| collection | DOAJ |
| description | Although microRNAs (miRNAs) have emerged as increasingly important target analytes, their biorecognition remains challenging due to their small size, high sequence homology, and low abundance in clinical samples. Nanospheres and microspheres have also gained increasing attention in biosensor applications due to their high specific surface area and the wide variety of compositions available. In this study, chemically designed and synthesized microspheres with active functional groups were used to promote effective miRNA immobilization resulting in better biorecognition. Upon conjugation with fluorescence-labeled complimentary probes, acylate-based spheres have indirectly detected MiR159, offering significantly enhanced analytical sensitivity, specificity, and accuracy while yielding a considerably low limit of detection (LOD) of 40 picomolar. Furthermore, MiR159 presence, which is known to be inversely correlated to breast cancer incidence and progression, was successfully detected in a competitive assay, which is promising for upgrading the current assay to clinical use. |
| format | Article |
| id | doaj-art-c1448ab5f284464fa9f9819b539d04ee |
| institution | OA Journals |
| issn | 0730-6679 1098-2329 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Polymer Technology |
| spelling | doaj-art-c1448ab5f284464fa9f9819b539d04ee2025-08-20T02:19:23ZengWileyAdvances in Polymer Technology0730-66791098-23292020-01-01202010.1155/2020/73106577310657Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA DetectionSamira Hosseini0Patricia Vázquez-Villegas1Richard C. Willson2Marco Rito-Palomares3Margarita Sanchez-Dominguez4Leo H. Koole5Marc J. Madou6Sergio O. Martínez-Chapa7Escuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, MexicoEscuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, MexicoUniversity of Houston, Department of Chemical and Biomolecular Engineering, Houston, TX 77204, USAEscuela de Medicina y Ciencias de Salud, Tecnologico de Monterrey, Avenida Ignacio Morones Prieto 3000 Poniente, 64710 Monterrey, NL, MexicoCentro de Investigación en Materiales Avanzados, S. C. (CIMAV), Unidad Monterrey, Parque de Investigación e Innovación Tecnológica, Apodaca, NL 66628, MexicoCentro de Investigación en Materiales Avanzados, S. C. (CIMAV), Unidad Monterrey, Parque de Investigación e Innovación Tecnológica, Apodaca, NL 66628, MexicoFaculty of Health, Medicine and Life Sciences, Maastricht University, Maastricht, NetherlandsEscuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, MexicoAlthough microRNAs (miRNAs) have emerged as increasingly important target analytes, their biorecognition remains challenging due to their small size, high sequence homology, and low abundance in clinical samples. Nanospheres and microspheres have also gained increasing attention in biosensor applications due to their high specific surface area and the wide variety of compositions available. In this study, chemically designed and synthesized microspheres with active functional groups were used to promote effective miRNA immobilization resulting in better biorecognition. Upon conjugation with fluorescence-labeled complimentary probes, acylate-based spheres have indirectly detected MiR159, offering significantly enhanced analytical sensitivity, specificity, and accuracy while yielding a considerably low limit of detection (LOD) of 40 picomolar. Furthermore, MiR159 presence, which is known to be inversely correlated to breast cancer incidence and progression, was successfully detected in a competitive assay, which is promising for upgrading the current assay to clinical use.http://dx.doi.org/10.1155/2020/7310657 |
| spellingShingle | Samira Hosseini Patricia Vázquez-Villegas Richard C. Willson Marco Rito-Palomares Margarita Sanchez-Dominguez Leo H. Koole Marc J. Madou Sergio O. Martínez-Chapa Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection Advances in Polymer Technology |
| title | Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection |
| title_full | Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection |
| title_fullStr | Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection |
| title_full_unstemmed | Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection |
| title_short | Polymethacrylate Sphere-Based Assay for Ultrasensitive miRNA Detection |
| title_sort | polymethacrylate sphere based assay for ultrasensitive mirna detection |
| url | http://dx.doi.org/10.1155/2020/7310657 |
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