Anticonvulsant Effect of Quinic Acid in Pentylenetetrazol-induced Seizures in Mice: Investigating the Role of Oxidative Stress Pathway and Nitric Oxide
Background and Aim: Epilepsy is a central nervous system disorder in which the activity of nerve cells in the brain is disrupted and leads to seizures. Despite the variety of available anticonvulsant drugs, approximately 30% of patients are resistant to conventional therapies. Nowadays, attention to...
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Kurdistan University of Medical Sciences
2025-03-01
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| Series: | مجله علمی دانشگاه علوم پزشکی کردستان |
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| Online Access: | http://sjku.muk.ac.ir/article-1-8310-en.pdf |
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| author | Behnam Raeisi Hossein Amini-Khoei Seyed Mohsen Mortazavi Mohammad Rahimi-Madiseh Zahra Lorigooini |
| author_facet | Behnam Raeisi Hossein Amini-Khoei Seyed Mohsen Mortazavi Mohammad Rahimi-Madiseh Zahra Lorigooini |
| author_sort | Behnam Raeisi |
| collection | DOAJ |
| description | Background and Aim: Epilepsy is a central nervous system disorder in which the activity of nerve cells in the brain is disrupted and leads to seizures. Despite the variety of available anticonvulsant drugs, approximately 30% of patients are resistant to conventional therapies. Nowadays, attention to drugs of natural origin, especially plant sources, has increased. Due to the effects of quinic acid, including antioxidant, anti-inflammatory, hepatoprotective, and immune cell-enhancing properties, this study aimed to determine the anticonvulsant effect of quinic acid in pentylenetetrazole-induced seizures in mice by investigating the possible role of the oxidative stress pathway and nitric oxide.
Materials and Methods: In this experimental study, 48 male mice were divided into 6 groups of 8. The first group received PTZ at a dose of 90 mg/kg, the second to fifth groups received quinic acid at doses of 5, 10, 20, and 40 mg/kg, and the sixth group received Diazepam at a dose of 10 mg/kg. One week after quinic acid administration, seizures were induced in all mice by intravenous injection of pentylenetetrazole.
Results: Quinic acid at doses of 20 and 40 mg/kg significantly increased seizure threshold compared to the group treated with normal saline (P<0.001). Quinicic acid treatment reduced serum nitrite and malondialdehyde levels of brain tissue (P<0.001). Quinic acid treatment also increased serum and brain tissue antioxidant activity in mice. Quinic acid significantly reduced the expression of iNOS and nNOS genes compared to the normal saline group (P<0.01).
Conclusion: Quinic acid seems to have potential anticonvulsant effects. Its mechanism of action is probably to inhibit neuroinflammation and act through the nitric oxide pathway |
| format | Article |
| id | doaj-art-c142411082704d819638c9a6bb47b3e5 |
| institution | DOAJ |
| issn | 1560-652X 2345-4040 |
| language | fas |
| publishDate | 2025-03-01 |
| publisher | Kurdistan University of Medical Sciences |
| record_format | Article |
| series | مجله علمی دانشگاه علوم پزشکی کردستان |
| spelling | doaj-art-c142411082704d819638c9a6bb47b3e52025-08-20T03:09:48ZfasKurdistan University of Medical Sciencesمجله علمی دانشگاه علوم پزشکی کردستان1560-652X2345-40402025-03-013011230Anticonvulsant Effect of Quinic Acid in Pentylenetetrazol-induced Seizures in Mice: Investigating the Role of Oxidative Stress Pathway and Nitric OxideBehnam Raeisi0Hossein Amini-Khoei1Seyed Mohsen Mortazavi2Mohammad Rahimi-Madiseh3Zahra Lorigooini4 MD Student, Student Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran Associate Professor, Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran Staff, Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran Associate Professor, Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran Associate Professor, Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran Background and Aim: Epilepsy is a central nervous system disorder in which the activity of nerve cells in the brain is disrupted and leads to seizures. Despite the variety of available anticonvulsant drugs, approximately 30% of patients are resistant to conventional therapies. Nowadays, attention to drugs of natural origin, especially plant sources, has increased. Due to the effects of quinic acid, including antioxidant, anti-inflammatory, hepatoprotective, and immune cell-enhancing properties, this study aimed to determine the anticonvulsant effect of quinic acid in pentylenetetrazole-induced seizures in mice by investigating the possible role of the oxidative stress pathway and nitric oxide. Materials and Methods: In this experimental study, 48 male mice were divided into 6 groups of 8. The first group received PTZ at a dose of 90 mg/kg, the second to fifth groups received quinic acid at doses of 5, 10, 20, and 40 mg/kg, and the sixth group received Diazepam at a dose of 10 mg/kg. One week after quinic acid administration, seizures were induced in all mice by intravenous injection of pentylenetetrazole. Results: Quinic acid at doses of 20 and 40 mg/kg significantly increased seizure threshold compared to the group treated with normal saline (P<0.001). Quinicic acid treatment reduced serum nitrite and malondialdehyde levels of brain tissue (P<0.001). Quinic acid treatment also increased serum and brain tissue antioxidant activity in mice. Quinic acid significantly reduced the expression of iNOS and nNOS genes compared to the normal saline group (P<0.01). Conclusion: Quinic acid seems to have potential anticonvulsant effects. Its mechanism of action is probably to inhibit neuroinflammation and act through the nitric oxide pathwayhttp://sjku.muk.ac.ir/article-1-8310-en.pdfseizurequinic acidpentylenetetrazoleoxidative stressnitric oxide |
| spellingShingle | Behnam Raeisi Hossein Amini-Khoei Seyed Mohsen Mortazavi Mohammad Rahimi-Madiseh Zahra Lorigooini Anticonvulsant Effect of Quinic Acid in Pentylenetetrazol-induced Seizures in Mice: Investigating the Role of Oxidative Stress Pathway and Nitric Oxide مجله علمی دانشگاه علوم پزشکی کردستان seizure quinic acid pentylenetetrazole oxidative stress nitric oxide |
| title | Anticonvulsant Effect of Quinic Acid in Pentylenetetrazol-induced Seizures in Mice: Investigating the Role of Oxidative Stress Pathway and Nitric Oxide |
| title_full | Anticonvulsant Effect of Quinic Acid in Pentylenetetrazol-induced Seizures in Mice: Investigating the Role of Oxidative Stress Pathway and Nitric Oxide |
| title_fullStr | Anticonvulsant Effect of Quinic Acid in Pentylenetetrazol-induced Seizures in Mice: Investigating the Role of Oxidative Stress Pathway and Nitric Oxide |
| title_full_unstemmed | Anticonvulsant Effect of Quinic Acid in Pentylenetetrazol-induced Seizures in Mice: Investigating the Role of Oxidative Stress Pathway and Nitric Oxide |
| title_short | Anticonvulsant Effect of Quinic Acid in Pentylenetetrazol-induced Seizures in Mice: Investigating the Role of Oxidative Stress Pathway and Nitric Oxide |
| title_sort | anticonvulsant effect of quinic acid in pentylenetetrazol induced seizures in mice investigating the role of oxidative stress pathway and nitric oxide |
| topic | seizure quinic acid pentylenetetrazole oxidative stress nitric oxide |
| url | http://sjku.muk.ac.ir/article-1-8310-en.pdf |
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