New RP-HPLC Method Development and Validation for Dorzolamide in Ophthalmic Dosage Form

A reversed phase liquid chromatographic method with UV detection at 254 nm for dorzolamide assay in ophthalmic solutions was developed and validated. Chromatographic separation was achieved on a Zorbax SB C18 (250 mm × 4.6 mm, 5 µm) column kept at 30°C with an isocratic mixture of mobile phase (phos...

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Main Authors: Boovizhikannan Thangabalan, Getu Kahsay, Tadele Eticha
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2018/4596141
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author Boovizhikannan Thangabalan
Getu Kahsay
Tadele Eticha
author_facet Boovizhikannan Thangabalan
Getu Kahsay
Tadele Eticha
author_sort Boovizhikannan Thangabalan
collection DOAJ
description A reversed phase liquid chromatographic method with UV detection at 254 nm for dorzolamide assay in ophthalmic solutions was developed and validated. Chromatographic separation was achieved on a Zorbax SB C18 (250 mm × 4.6 mm, 5 µm) column kept at 30°C with an isocratic mixture of mobile phase (phosphate buffer, pH 2.5, and acetonitrile, 90 : 10 v/v) at a flow rate of 0.8 mL/min. The method was validated for its specificity, linearity, accuracy, precision, limit of detection, limit of quantification, and robustness based on ICH guidelines. The validation studies revealed satisfactory results. The proposed method has been applied for the quantification of dorzolamide in commercial samples. The developed method is fast, simple, specific, accurate, and sensitive, hence can be applied for routine quality control analysis of dorzolamide in pharmaceutical dosage form.
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institution Kabale University
issn 2090-8865
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language English
publishDate 2018-01-01
publisher Wiley
record_format Article
series Journal of Analytical Methods in Chemistry
spelling doaj-art-c13d63d233fc4e59abdaf1fcef61d71e2025-02-03T06:13:15ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732018-01-01201810.1155/2018/45961414596141New RP-HPLC Method Development and Validation for Dorzolamide in Ophthalmic Dosage FormBoovizhikannan Thangabalan0Getu Kahsay1Tadele Eticha2School of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaSchool of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaA reversed phase liquid chromatographic method with UV detection at 254 nm for dorzolamide assay in ophthalmic solutions was developed and validated. Chromatographic separation was achieved on a Zorbax SB C18 (250 mm × 4.6 mm, 5 µm) column kept at 30°C with an isocratic mixture of mobile phase (phosphate buffer, pH 2.5, and acetonitrile, 90 : 10 v/v) at a flow rate of 0.8 mL/min. The method was validated for its specificity, linearity, accuracy, precision, limit of detection, limit of quantification, and robustness based on ICH guidelines. The validation studies revealed satisfactory results. The proposed method has been applied for the quantification of dorzolamide in commercial samples. The developed method is fast, simple, specific, accurate, and sensitive, hence can be applied for routine quality control analysis of dorzolamide in pharmaceutical dosage form.http://dx.doi.org/10.1155/2018/4596141
spellingShingle Boovizhikannan Thangabalan
Getu Kahsay
Tadele Eticha
New RP-HPLC Method Development and Validation for Dorzolamide in Ophthalmic Dosage Form
Journal of Analytical Methods in Chemistry
title New RP-HPLC Method Development and Validation for Dorzolamide in Ophthalmic Dosage Form
title_full New RP-HPLC Method Development and Validation for Dorzolamide in Ophthalmic Dosage Form
title_fullStr New RP-HPLC Method Development and Validation for Dorzolamide in Ophthalmic Dosage Form
title_full_unstemmed New RP-HPLC Method Development and Validation for Dorzolamide in Ophthalmic Dosage Form
title_short New RP-HPLC Method Development and Validation for Dorzolamide in Ophthalmic Dosage Form
title_sort new rp hplc method development and validation for dorzolamide in ophthalmic dosage form
url http://dx.doi.org/10.1155/2018/4596141
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AT tadeleeticha newrphplcmethoddevelopmentandvalidationfordorzolamideinophthalmicdosageform