Association of eNOS Gene Polymorphisms G894T and T-786C with Risk of Hepatorenal Syndrome
Background. There are no studies investigating the relationship between endothelial nitric oxide synthase (eNOS) gene polymorphisms and hepatorenal syndrome (HRS). Aim. The purpose of this study is to elucidate whether eNOS gene polymorphisms (G894T and T-786C) play a role in the development of type...
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| Format: | Article |
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Wiley
2016-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2016/2579626 |
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| author | Yuksel Seckin Ali Yigit Elif Yesilada Gonca Gulbay Yasir Furkan Cagin Harika Gozukara Yılmaz Bılgıc Oguzhan Yildirim Yusuf Turkoz Zeynep Aksungur |
| author_facet | Yuksel Seckin Ali Yigit Elif Yesilada Gonca Gulbay Yasir Furkan Cagin Harika Gozukara Yılmaz Bılgıc Oguzhan Yildirim Yusuf Turkoz Zeynep Aksungur |
| author_sort | Yuksel Seckin |
| collection | DOAJ |
| description | Background. There are no studies investigating the relationship between endothelial nitric oxide synthase (eNOS) gene polymorphisms and hepatorenal syndrome (HRS). Aim. The purpose of this study is to elucidate whether eNOS gene polymorphisms (G894T and T-786C) play a role in the development of type-2 HRS. Methods. This study was carried out in a group of 92 patients with cirrhosis (44 patients with type-2 HRS and 48 without HRS) and 50 healthy controls. Polymorphisms were determined by polymerase chain reaction (PCR) and melting curve analysis. Results. We did not find any significant difference in allele and genotype distributions of the eNOS -T-786C polymorphism among the groups (p=0.440). However, the frequency of GT (40.9%) and TT (13.6%) genotypes and mutant allele T (34.1%) for the eNOS G894T polymorphism were significantly higher (p<0.001 and p<0.001, resp.) in the HRS group than in both the stable cirrhosis (14.6%, 4.2%, and 11.5%, resp.) and the control (22.0%, 2.0%, and 13.0%, resp.) groups. Conclusion. The occurrence of mutant genotypes (GT/TT) and mutant allele T in eNOS -G894T polymorphisms should be considered as a potential risk factor in cirrhotic patients with HRS. |
| format | Article |
| id | doaj-art-c12a8a457c184b7583146de00b16a0b4 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-c12a8a457c184b7583146de00b16a0b42025-02-03T05:48:16ZengWileyGastroenterology Research and Practice1687-61211687-630X2016-01-01201610.1155/2016/25796262579626Association of eNOS Gene Polymorphisms G894T and T-786C with Risk of Hepatorenal SyndromeYuksel Seckin0Ali Yigit1Elif Yesilada2Gonca Gulbay3Yasir Furkan Cagin4Harika Gozukara5Yılmaz Bılgıc6Oguzhan Yildirim7Yusuf Turkoz8Zeynep Aksungur9Department of Gastroenterology, Faculty of Medicine, Inonu University, 44280 Malatya, TurkeyDepartment of Internal Medicine, Faculty of Medicine, Inonu University, 44280 Malatya, TurkeyDepartment of Medical Biology and Genetics, Faculty of Medicine, Inonu University, 44280 Malatya, TurkeyDepartment of Medical Biology and Genetics, Faculty of Medicine, Inonu University, 44280 Malatya, TurkeyDepartment of Gastroenterology, Faculty of Medicine, Inonu University, 44280 Malatya, TurkeyDepartment of Biostatistics, Faculty of Medicine, Inonu University, 44280 Malatya, TurkeyDepartment of Gastroenterology, Faculty of Medicine, Inonu University, 44280 Malatya, TurkeyDepartment of Gastroenterology, Faculty of Medicine, Inonu University, 44280 Malatya, TurkeyDepartment of Biochemistry, Faculty of Medicine, Inonu University, 44280 Malatya, TurkeyDepartment of Biochemistry, Faculty of Medicine, Inonu University, 44280 Malatya, TurkeyBackground. There are no studies investigating the relationship between endothelial nitric oxide synthase (eNOS) gene polymorphisms and hepatorenal syndrome (HRS). Aim. The purpose of this study is to elucidate whether eNOS gene polymorphisms (G894T and T-786C) play a role in the development of type-2 HRS. Methods. This study was carried out in a group of 92 patients with cirrhosis (44 patients with type-2 HRS and 48 without HRS) and 50 healthy controls. Polymorphisms were determined by polymerase chain reaction (PCR) and melting curve analysis. Results. We did not find any significant difference in allele and genotype distributions of the eNOS -T-786C polymorphism among the groups (p=0.440). However, the frequency of GT (40.9%) and TT (13.6%) genotypes and mutant allele T (34.1%) for the eNOS G894T polymorphism were significantly higher (p<0.001 and p<0.001, resp.) in the HRS group than in both the stable cirrhosis (14.6%, 4.2%, and 11.5%, resp.) and the control (22.0%, 2.0%, and 13.0%, resp.) groups. Conclusion. The occurrence of mutant genotypes (GT/TT) and mutant allele T in eNOS -G894T polymorphisms should be considered as a potential risk factor in cirrhotic patients with HRS.http://dx.doi.org/10.1155/2016/2579626 |
| spellingShingle | Yuksel Seckin Ali Yigit Elif Yesilada Gonca Gulbay Yasir Furkan Cagin Harika Gozukara Yılmaz Bılgıc Oguzhan Yildirim Yusuf Turkoz Zeynep Aksungur Association of eNOS Gene Polymorphisms G894T and T-786C with Risk of Hepatorenal Syndrome Gastroenterology Research and Practice |
| title | Association of eNOS Gene Polymorphisms G894T and T-786C with Risk of Hepatorenal Syndrome |
| title_full | Association of eNOS Gene Polymorphisms G894T and T-786C with Risk of Hepatorenal Syndrome |
| title_fullStr | Association of eNOS Gene Polymorphisms G894T and T-786C with Risk of Hepatorenal Syndrome |
| title_full_unstemmed | Association of eNOS Gene Polymorphisms G894T and T-786C with Risk of Hepatorenal Syndrome |
| title_short | Association of eNOS Gene Polymorphisms G894T and T-786C with Risk of Hepatorenal Syndrome |
| title_sort | association of enos gene polymorphisms g894t and t 786c with risk of hepatorenal syndrome |
| url | http://dx.doi.org/10.1155/2016/2579626 |
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