Computational Design and Bioinformatic Insights into Recombinant Whey Protein Production
The rising global demand for sustainable protein sources highlights the need for alternatives to traditional whey protein production, which is resource-intensive and environmentally taxing. This study presents an in-silico approach for producing recombinant whey proteins—α-lactalbumin and β-lactogl...
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| Format: | Article |
| Language: | English |
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Biotechnology Society of Nepal
2025-07-01
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| Series: | Nepal Journal of Biotechnology |
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| Online Access: | https://nepjb.com/index.php/NJB/article/view/380 |
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| author | Aathish Gurunathan Harini Subalakshmi M |
| author_facet | Aathish Gurunathan Harini Subalakshmi M |
| author_sort | Aathish Gurunathan |
| collection | DOAJ |
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The rising global demand for sustainable protein sources highlights the need for alternatives to traditional whey protein production, which is resource-intensive and environmentally taxing. This study presents an in-silico approach for producing recombinant whey proteins—α-lactalbumin and β-lactoglobulin—using Saccharomyces cerevisiae as a host. Codon-optimized gene constructs were designed and validated through computational tools, showing enhanced translational efficiency, structural stability, and functional potential. Allergenicity analysis revealed moderate risk, suggesting a need for further refinement. Overall, the findings demonstrate the feasibility of precision fermentation for scalable, eco-friendly whey protein production, with broad applications in functional foods and biotechnology.
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| format | Article |
| id | doaj-art-c122f55ced774451bce431996790d2c3 |
| institution | Kabale University |
| issn | 2091-1130 2467-9313 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Biotechnology Society of Nepal |
| record_format | Article |
| series | Nepal Journal of Biotechnology |
| spelling | doaj-art-c122f55ced774451bce431996790d2c32025-08-20T03:36:34ZengBiotechnology Society of NepalNepal Journal of Biotechnology2091-11302467-93132025-07-0113110.54796/njb.v13i1.380Computational Design and Bioinformatic Insights into Recombinant Whey Protein Production Aathish Gurunathan0https://orcid.org/0009-0004-7307-0779Harini Subalakshmi M1https://orcid.org/0009-0000-2748-8331Department of Industrial Biotechnology, Government College of Technology, Coimbatore, Tamil Nadu, IndiaDepartment of Industrial Biotechnology, Government College of Technology, Coimbatore, Tamil Nadu, India The rising global demand for sustainable protein sources highlights the need for alternatives to traditional whey protein production, which is resource-intensive and environmentally taxing. This study presents an in-silico approach for producing recombinant whey proteins—α-lactalbumin and β-lactoglobulin—using Saccharomyces cerevisiae as a host. Codon-optimized gene constructs were designed and validated through computational tools, showing enhanced translational efficiency, structural stability, and functional potential. Allergenicity analysis revealed moderate risk, suggesting a need for further refinement. Overall, the findings demonstrate the feasibility of precision fermentation for scalable, eco-friendly whey protein production, with broad applications in functional foods and biotechnology. https://nepjb.com/index.php/NJB/article/view/380Whey proteinIn silico methodRecombinant plasmid constructionSaccharomyces cerevisiaeα-lactalbuminβ- lactoglobulin |
| spellingShingle | Aathish Gurunathan Harini Subalakshmi M Computational Design and Bioinformatic Insights into Recombinant Whey Protein Production Nepal Journal of Biotechnology Whey protein In silico method Recombinant plasmid construction Saccharomyces cerevisiae α-lactalbumin β- lactoglobulin |
| title | Computational Design and Bioinformatic Insights into Recombinant Whey Protein Production |
| title_full | Computational Design and Bioinformatic Insights into Recombinant Whey Protein Production |
| title_fullStr | Computational Design and Bioinformatic Insights into Recombinant Whey Protein Production |
| title_full_unstemmed | Computational Design and Bioinformatic Insights into Recombinant Whey Protein Production |
| title_short | Computational Design and Bioinformatic Insights into Recombinant Whey Protein Production |
| title_sort | computational design and bioinformatic insights into recombinant whey protein production |
| topic | Whey protein In silico method Recombinant plasmid construction Saccharomyces cerevisiae α-lactalbumin β- lactoglobulin |
| url | https://nepjb.com/index.php/NJB/article/view/380 |
| work_keys_str_mv | AT aathishgurunathan computationaldesignandbioinformaticinsightsintorecombinantwheyproteinproduction AT harinisubalakshmim computationaldesignandbioinformaticinsightsintorecombinantwheyproteinproduction |