Inhibition of cancer cells using target-specific 2A3 antibody-conjugated gold nanoclusters
Background: Metal nanoclusters (NCs) with outstanding structural and optical properties have been intensively validated for applications in nanomedicine and nanotechnology. Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is overexpressed in many cancer cells. Objective: The g...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Biochemistry and Molecular Biology
2022-03-01
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| Series: | Acta Biochimica Indonesiana |
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| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/69 |
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| author | Jui-Chi Kuo Tsung-Rong Kuo Fajar Rinawati Erna Susilowati Sucipto Dyah Ika Krisnawati |
| author_facet | Jui-Chi Kuo Tsung-Rong Kuo Fajar Rinawati Erna Susilowati Sucipto Dyah Ika Krisnawati |
| author_sort | Jui-Chi Kuo |
| collection | DOAJ |
| description |
Background: Metal nanoclusters (NCs) with outstanding structural and optical properties have been intensively validated for applications in nanomedicine and nanotechnology. Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is overexpressed in many cancer cells.
Objective: The gold nanoclusters conjugated with a single domain antibody targeting CEACAM6 of 2A3 (2A3-AuNCs) were synthesized for the inhibition of cancer cells.
Methods: 2A3-AuNCs were prepared via a facile hydrothermal approach. The cell viability was measured by resazurin dye reduction assay. The cell death was analyzed by fluorescence imaging.
Results: Structural and optical characterizations demonstrated the successful synthesis of 2A3-AuNCs with a roughly spherical shape and a size of 2.35 nm. The 2A3-AuNCs revealed a maximum fluorescence intensity at 350 nm with a fluorescence quantum yield of 4.0%. The cell viability assay indicated that 2A3-AuNCs could inhibit the growths of cancer cells with overexpressed CEACAM6, including breast cancer MDA-MB-231 and MDA-MB-468 cells. The fluorescence imaging results also demonstrated that 2A3-AuNCs could inhibit the growth of cancer cells with MDA-MB-231 and MDA-MB-468 cells.
Conclusion: Combination with the results of cell viability assay and fluorescence imaging, the surface ligand of 2A3 antibody on 2A3-AuNCs exhibited promising inhibition of CEACAM6 overexpressed cancer cells. Our work provides a potential application of AuNCs in cancer therapy.
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| format | Article |
| id | doaj-art-c1220c901f5247e5955cba7b46c8ed6e |
| institution | Kabale University |
| issn | 2654-6108 2654-3222 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj-art-c1220c901f5247e5955cba7b46c8ed6e2025-02-08T03:06:05ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222022-03-014210.32889/actabioina.69Inhibition of cancer cells using target-specific 2A3 antibody-conjugated gold nanoclustersJui-Chi Kuo0Tsung-Rong Kuo1Fajar Rinawati2Erna Susilowati3Sucipto4Dyah Ika Krisnawati5Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, TaiwanInternational Ph.D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11031, TaiwanDharma Husada Nursing Academy, Kediri, 64114, IndonesiaDharma Husada Nursing Academy, Kediri, 64114, IndonesiaDharma Husada Nursing Academy, Kediri, 64114, IndonesiaDharma Husada Nursing Academy, Kediri 64114, Indonesia Background: Metal nanoclusters (NCs) with outstanding structural and optical properties have been intensively validated for applications in nanomedicine and nanotechnology. Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is overexpressed in many cancer cells. Objective: The gold nanoclusters conjugated with a single domain antibody targeting CEACAM6 of 2A3 (2A3-AuNCs) were synthesized for the inhibition of cancer cells. Methods: 2A3-AuNCs were prepared via a facile hydrothermal approach. The cell viability was measured by resazurin dye reduction assay. The cell death was analyzed by fluorescence imaging. Results: Structural and optical characterizations demonstrated the successful synthesis of 2A3-AuNCs with a roughly spherical shape and a size of 2.35 nm. The 2A3-AuNCs revealed a maximum fluorescence intensity at 350 nm with a fluorescence quantum yield of 4.0%. The cell viability assay indicated that 2A3-AuNCs could inhibit the growths of cancer cells with overexpressed CEACAM6, including breast cancer MDA-MB-231 and MDA-MB-468 cells. The fluorescence imaging results also demonstrated that 2A3-AuNCs could inhibit the growth of cancer cells with MDA-MB-231 and MDA-MB-468 cells. Conclusion: Combination with the results of cell viability assay and fluorescence imaging, the surface ligand of 2A3 antibody on 2A3-AuNCs exhibited promising inhibition of CEACAM6 overexpressed cancer cells. Our work provides a potential application of AuNCs in cancer therapy. https://pbbmi.org/newjurnal/index.php/actabioina/article/view/692A3CEACAM6gold nanoclustersinhibitiontherapy |
| spellingShingle | Jui-Chi Kuo Tsung-Rong Kuo Fajar Rinawati Erna Susilowati Sucipto Dyah Ika Krisnawati Inhibition of cancer cells using target-specific 2A3 antibody-conjugated gold nanoclusters Acta Biochimica Indonesiana 2A3 CEACAM6 gold nanoclusters inhibition therapy |
| title | Inhibition of cancer cells using target-specific 2A3 antibody-conjugated gold nanoclusters |
| title_full | Inhibition of cancer cells using target-specific 2A3 antibody-conjugated gold nanoclusters |
| title_fullStr | Inhibition of cancer cells using target-specific 2A3 antibody-conjugated gold nanoclusters |
| title_full_unstemmed | Inhibition of cancer cells using target-specific 2A3 antibody-conjugated gold nanoclusters |
| title_short | Inhibition of cancer cells using target-specific 2A3 antibody-conjugated gold nanoclusters |
| title_sort | inhibition of cancer cells using target specific 2a3 antibody conjugated gold nanoclusters |
| topic | 2A3 CEACAM6 gold nanoclusters inhibition therapy |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/69 |
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