Gelatin/polycaprolactone membranes promote endometrial regeneration and restore fertility
Objective To investigate the effectiveness of gelatin/polycaprolactone (GT/PCL) membranes for restoring endometrial structure and function and fertility in a rat model of endometrial injury.Methods We randomized 125 female Sprague-Dawley (SD) rats to the sham, natural repair (NR), estrogen (E), GT/P...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Gynecological Endocrinology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/09513590.2024.2442728 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850243243865800704 |
|---|---|
| author | Xin Wang Chun-run Yang Zhen-dan Zhao De-gao Liu Chang-zhong Li |
| author_facet | Xin Wang Chun-run Yang Zhen-dan Zhao De-gao Liu Chang-zhong Li |
| author_sort | Xin Wang |
| collection | DOAJ |
| description | Objective To investigate the effectiveness of gelatin/polycaprolactone (GT/PCL) membranes for restoring endometrial structure and function and fertility in a rat model of endometrial injury.Methods We randomized 125 female Sprague-Dawley (SD) rats to the sham, natural repair (NR), estrogen (E), GT/PCL, and E-GT/PCL groups. Except for the sham group, all rats underwent uterine curettage. After 1, 2, 3, and 4 weeks, 12 rats from each group were sacrificed; their uterine tissue was collected for histological, immunohistochemical, and reverse transcription quantitative-polymerase chain reaction analyses. Eight rats from each group underwent radiography of the uterine cavity. The remaining 25 females were mated with males to assess fertility 60 d postoperatively.Results The GT/PCL and E-GT/PCL groups had higher endometrial thickness, collagen degradation, cytokeratin 19, vimentin (VIM) expression, and microvessel densities and had higher levels of estrogen receptors (ERα) but lower levels of tumor necrosis factor (TNF) than the NR and E groups. They also showed better hysterographic patency, with the shape of the uterine cavity similar to that of the sham group, and higher embryo implantation rates than the NR and E groups.Conclusions GT/PCL membranes promote endometrial regeneration and improve fertility in rats. They may help prevent intrauterine adhesions (IUAs) postoperatively and warrant further investigation. |
| format | Article |
| id | doaj-art-c1202d0b92f342ce9fa564a9bdc448f3 |
| institution | OA Journals |
| issn | 0951-3590 1473-0766 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gynecological Endocrinology |
| spelling | doaj-art-c1202d0b92f342ce9fa564a9bdc448f32025-08-20T02:00:03ZengTaylor & Francis GroupGynecological Endocrinology0951-35901473-07662024-12-0140110.1080/09513590.2024.2442728Gelatin/polycaprolactone membranes promote endometrial regeneration and restore fertilityXin Wang0Chun-run Yang1Zhen-dan Zhao2De-gao Liu3Chang-zhong Li4Shandong Provincial Hospital, Shandong University, Jinan, Shandong, ChinaShandong Provincial Hospital, Shandong University, Jinan, Shandong, ChinaShandong Provincial Hospital, Shandong University, Jinan, Shandong, ChinaInstitute of Obstetrics and Gynecology, Shenzhen PKU-HKUST Medical Center, Shenzhen, Guangdong, ChinaShandong Provincial Hospital, Shandong University, Jinan, Shandong, ChinaObjective To investigate the effectiveness of gelatin/polycaprolactone (GT/PCL) membranes for restoring endometrial structure and function and fertility in a rat model of endometrial injury.Methods We randomized 125 female Sprague-Dawley (SD) rats to the sham, natural repair (NR), estrogen (E), GT/PCL, and E-GT/PCL groups. Except for the sham group, all rats underwent uterine curettage. After 1, 2, 3, and 4 weeks, 12 rats from each group were sacrificed; their uterine tissue was collected for histological, immunohistochemical, and reverse transcription quantitative-polymerase chain reaction analyses. Eight rats from each group underwent radiography of the uterine cavity. The remaining 25 females were mated with males to assess fertility 60 d postoperatively.Results The GT/PCL and E-GT/PCL groups had higher endometrial thickness, collagen degradation, cytokeratin 19, vimentin (VIM) expression, and microvessel densities and had higher levels of estrogen receptors (ERα) but lower levels of tumor necrosis factor (TNF) than the NR and E groups. They also showed better hysterographic patency, with the shape of the uterine cavity similar to that of the sham group, and higher embryo implantation rates than the NR and E groups.Conclusions GT/PCL membranes promote endometrial regeneration and improve fertility in rats. They may help prevent intrauterine adhesions (IUAs) postoperatively and warrant further investigation.https://www.tandfonline.com/doi/10.1080/09513590.2024.2442728Endometriumfertilitygelatin/polycaprolactone membraneintrauterine adhesionsSprague-Dawley rats |
| spellingShingle | Xin Wang Chun-run Yang Zhen-dan Zhao De-gao Liu Chang-zhong Li Gelatin/polycaprolactone membranes promote endometrial regeneration and restore fertility Gynecological Endocrinology Endometrium fertility gelatin/polycaprolactone membrane intrauterine adhesions Sprague-Dawley rats |
| title | Gelatin/polycaprolactone membranes promote endometrial regeneration and restore fertility |
| title_full | Gelatin/polycaprolactone membranes promote endometrial regeneration and restore fertility |
| title_fullStr | Gelatin/polycaprolactone membranes promote endometrial regeneration and restore fertility |
| title_full_unstemmed | Gelatin/polycaprolactone membranes promote endometrial regeneration and restore fertility |
| title_short | Gelatin/polycaprolactone membranes promote endometrial regeneration and restore fertility |
| title_sort | gelatin polycaprolactone membranes promote endometrial regeneration and restore fertility |
| topic | Endometrium fertility gelatin/polycaprolactone membrane intrauterine adhesions Sprague-Dawley rats |
| url | https://www.tandfonline.com/doi/10.1080/09513590.2024.2442728 |
| work_keys_str_mv | AT xinwang gelatinpolycaprolactonemembranespromoteendometrialregenerationandrestorefertility AT chunrunyang gelatinpolycaprolactonemembranespromoteendometrialregenerationandrestorefertility AT zhendanzhao gelatinpolycaprolactonemembranespromoteendometrialregenerationandrestorefertility AT degaoliu gelatinpolycaprolactonemembranespromoteendometrialregenerationandrestorefertility AT changzhongli gelatinpolycaprolactonemembranespromoteendometrialregenerationandrestorefertility |