Analysis of the efficacy of MALDI-TOF MS technology in identifying microorganisms in cancer patients and oncology hospital environment

Rapid diagnostics of microbes in hospitals are crucial for promptly identifying infections, enabling timely and appropriate treatment. The study was conducted to evaluate the effectiveness of the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) technology i...

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Main Authors: Grażyna Czeszewska-Rosiak, Iwona Adamczyk, Agnieszka Ludwiczak, Piotr Fijałkowski, Paweł Fijałkowski, Magdalena Twarużek, Michał Złoch, Dorota Gabryś, Wioletta Miśta, Andrzej Tretyn, Paweł Piotr Pomastowski
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Heliyon
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405844025003950
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author Grażyna Czeszewska-Rosiak
Iwona Adamczyk
Agnieszka Ludwiczak
Piotr Fijałkowski
Paweł Fijałkowski
Magdalena Twarużek
Michał Złoch
Dorota Gabryś
Wioletta Miśta
Andrzej Tretyn
Paweł Piotr Pomastowski
author_facet Grażyna Czeszewska-Rosiak
Iwona Adamczyk
Agnieszka Ludwiczak
Piotr Fijałkowski
Paweł Fijałkowski
Magdalena Twarużek
Michał Złoch
Dorota Gabryś
Wioletta Miśta
Andrzej Tretyn
Paweł Piotr Pomastowski
author_sort Grażyna Czeszewska-Rosiak
collection DOAJ
description Rapid diagnostics of microbes in hospitals are crucial for promptly identifying infections, enabling timely and appropriate treatment. The study was conducted to evaluate the effectiveness of the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) technology in the microbial profiling of hospital environments and patient samples. The objective was to determine the microbial landscape in swabs collected from hospitalized patients and their immediate environments, using MALDI to compare the capabilities of two systems: BRUKER and ZYBIO. The analysis resulted in 1012 microbial identifications from patient samples (N = 81), encompassing 96 species, and 1496 identifications from hospital surface samples (N = 108), covering 124 species. Predominantly identified microorganisms in patients’ samples included Staphylococcus epidermidis, Staphylococcus aureus, Staphylococcus capitis, Steptococcus salivarius, and Micrococcus luteus, whereas environmental samples chiefly yielded S. epidermidis, Staphylococcus hominis, Staphylococcus warneri, and Microcccus luteus. 33 species were found in both types of samples, highlighting a significant microbial interchange within hospital settings. Both MALDI systems showed high consistency in results at both genus and species levels. Nevertheless, mismatches in identification between both MALDI systems were noted, particularly within Brevibacterium, Streptococcus, Bacillus, Staphylococcus, and Neisseria genera. This study presents the precision of MALDI technology in microbial identification and highlights the need for ongoing enhancements, especially in the expansion and updating of databases, to bolster its diagnostic effectiveness further.
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spelling doaj-art-c11ed361935d43238f5bd9b2e7bc89fa2025-02-02T05:28:48ZengElsevierHeliyon2405-84402025-01-01112e42015Analysis of the efficacy of MALDI-TOF MS technology in identifying microorganisms in cancer patients and oncology hospital environmentGrażyna Czeszewska-Rosiak0Iwona Adamczyk1Agnieszka Ludwiczak2Piotr Fijałkowski3Paweł Fijałkowski4Magdalena Twarużek5Michał Złoch6Dorota Gabryś7Wioletta Miśta8Andrzej Tretyn9Paweł Piotr Pomastowski10Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wilenska 4 Str., Torun 87-100, Poland; Departnemt of Plant Physiology and Biotechnology, Nicolaus Copernicus University in Torun, Lwowska 1 Str., 87-100 Torun, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wilenska 4 Str., Torun 87-100, Poland; Department of Physiology and Toxicology, Kazimierz Wielki University, Chodkiewicza 30 Str., Bydgoszcz, Poland; Corresponding author. Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wilenska 4 Str., Torun 87-100, Poland.Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wilenska 4 Str., Torun 87-100, Poland; Department of Immunology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Lwowska 1 Str., 87-100 Toruń, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wilenska 4 Str., Torun 87-100, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wilenska 4 Str., Torun 87-100, Poland; Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7 Str., 87-100 Toruń, PolandDepartment of Physiology and Toxicology, Kazimierz Wielki University, Chodkiewicza 30 Str., Bydgoszcz, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wilenska 4 Str., Torun 87-100, PolandRadiotherapy Department, Maria Sklodowska-Curie National Research Institute of Oncology, Wybrzeze Armii Krajowej 15 Str., PolandRadiotherapy Department, Maria Sklodowska-Curie National Research Institute of Oncology, Wybrzeze Armii Krajowej 15 Str., PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wilenska 4 Str., Torun 87-100, Poland; Departnemt of Plant Physiology and Biotechnology, Nicolaus Copernicus University in Torun, Lwowska 1 Str., 87-100 Torun, PolandCentre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Torun, Wilenska 4 Str., Torun 87-100, PolandRapid diagnostics of microbes in hospitals are crucial for promptly identifying infections, enabling timely and appropriate treatment. The study was conducted to evaluate the effectiveness of the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) technology in the microbial profiling of hospital environments and patient samples. The objective was to determine the microbial landscape in swabs collected from hospitalized patients and their immediate environments, using MALDI to compare the capabilities of two systems: BRUKER and ZYBIO. The analysis resulted in 1012 microbial identifications from patient samples (N = 81), encompassing 96 species, and 1496 identifications from hospital surface samples (N = 108), covering 124 species. Predominantly identified microorganisms in patients’ samples included Staphylococcus epidermidis, Staphylococcus aureus, Staphylococcus capitis, Steptococcus salivarius, and Micrococcus luteus, whereas environmental samples chiefly yielded S. epidermidis, Staphylococcus hominis, Staphylococcus warneri, and Microcccus luteus. 33 species were found in both types of samples, highlighting a significant microbial interchange within hospital settings. Both MALDI systems showed high consistency in results at both genus and species levels. Nevertheless, mismatches in identification between both MALDI systems were noted, particularly within Brevibacterium, Streptococcus, Bacillus, Staphylococcus, and Neisseria genera. This study presents the precision of MALDI technology in microbial identification and highlights the need for ongoing enhancements, especially in the expansion and updating of databases, to bolster its diagnostic effectiveness further.http://www.sciencedirect.com/science/article/pii/S2405844025003950MALDI-TOF MSMicroorganisms' identificationPathogen detectionOpportunistic bacteriaHealthcare environments
spellingShingle Grażyna Czeszewska-Rosiak
Iwona Adamczyk
Agnieszka Ludwiczak
Piotr Fijałkowski
Paweł Fijałkowski
Magdalena Twarużek
Michał Złoch
Dorota Gabryś
Wioletta Miśta
Andrzej Tretyn
Paweł Piotr Pomastowski
Analysis of the efficacy of MALDI-TOF MS technology in identifying microorganisms in cancer patients and oncology hospital environment
Heliyon
MALDI-TOF MS
Microorganisms' identification
Pathogen detection
Opportunistic bacteria
Healthcare environments
title Analysis of the efficacy of MALDI-TOF MS technology in identifying microorganisms in cancer patients and oncology hospital environment
title_full Analysis of the efficacy of MALDI-TOF MS technology in identifying microorganisms in cancer patients and oncology hospital environment
title_fullStr Analysis of the efficacy of MALDI-TOF MS technology in identifying microorganisms in cancer patients and oncology hospital environment
title_full_unstemmed Analysis of the efficacy of MALDI-TOF MS technology in identifying microorganisms in cancer patients and oncology hospital environment
title_short Analysis of the efficacy of MALDI-TOF MS technology in identifying microorganisms in cancer patients and oncology hospital environment
title_sort analysis of the efficacy of maldi tof ms technology in identifying microorganisms in cancer patients and oncology hospital environment
topic MALDI-TOF MS
Microorganisms' identification
Pathogen detection
Opportunistic bacteria
Healthcare environments
url http://www.sciencedirect.com/science/article/pii/S2405844025003950
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